2004
DOI: 10.1128/jvi.78.11.5891-5899.2004
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Antisense Morpholino-Oligomers Directed against the 5′ End of the Genome Inhibit Coronavirus Proliferation and Growth†

Abstract: Conjugation of a peptide related to the human immunodeficiency virus type 1 Tat represents a novel method for delivery of antisense morpholino-oligomers. Conjugated and unconjugated oligomers were tested to determine sequence-specific antiviral efficacy against a member of the Coronaviridae, Mouse hepatitis virus (MHV). Specific antisense activity designed to block translation of the viral replicase polyprotein was first confirmed by reduction of luciferase expression from a target sequence-containing reporter… Show more

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Cited by 71 publications
(78 citation statements)
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“…2C). Two conjugates were the most effective, namely, the R 9 F 2 peptide used above, which was previously employed for MHV and SARS-CoV antiviral studies (24,25), and a peptide consisting of four repeats of arginine-6-amino-hexanoic acid (X)-arginine [(RXR) 4 ] followed by either a cysteine (C) or another single X residue and a beta-alanine (B) to serve as respective linkers (Fig. 2C).…”
Section: Resultsmentioning
confidence: 99%
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“…2C). Two conjugates were the most effective, namely, the R 9 F 2 peptide used above, which was previously employed for MHV and SARS-CoV antiviral studies (24,25), and a peptide consisting of four repeats of arginine-6-amino-hexanoic acid (X)-arginine [(RXR) 4 ] followed by either a cysteine (C) or another single X residue and a beta-alanine (B) to serve as respective linkers (Fig. 2C).…”
Section: Resultsmentioning
confidence: 99%
“…Alb139 (5, 19) was obtained from P. Rottier. MHV stocks were produced on 17Cl-1 cells (MHV-1) or DBT cells (all other strains), and titers were determined by plaque assay as previously described (25). SARS-CoV-Tor2 was cultivated on Vero-E6 cells as previously described (24).…”
Section: Cells and Virusesmentioning
confidence: 99%
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“…The mechanism of action of these compounds is through stable and sequence-specific duplexing with RNA, thereby obstructing access of biomolecules to a particular sequence of RNA. PMOs have demonstrated effective and specific suppression of the replication of several RNA viruses (30,39). Conjugation of arginine-rich peptides to PMOs has been shown to greatly increase PMO cellular uptake and inhibitory efficacy against specific targets in cell culture systems compared to PMOs either lacking a peptide conjugate or conjugated with various other peptides (28,30).…”
mentioning
confidence: 99%