Antisense suppression of alcohol acetyltransferase gene in ripening melon fruit alters volatile composition

W, Shan; Zhao, Cong; Fan, Jianguang; Cong, Hongzi; Liang, Shicai; Xj, Yu

doi:10.1016/j.scienta.2012.03.010

Cited by 25 publications

(19 citation statements)

References 34 publications

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“…The activity of AAT in 1-MCP-treated fruit was significantly reduced compared with that in control fruit. However, the degree of suppression of enzymatic activity was less than that of the mRNA levels, suggesting that there are likely as yet unidentified AAT genes within the oriental sweet melon genome, which was in agreement with the work of Shan et al (2012). Changes in AAT activity in all treatments of 'Caihong7' and 'Tianbao' oriental sweet melon were not consistent with the pattern observed in AAT expression.…”

Section: Discussionsupporting

confidence: 64%

Role of Ethylene in the Biosynthetic Pathway of Related-aroma Volatiles Derived from Fatty Acids in Oriental Sweet Melon

Li¹,

Jin

et al. 2016

J. Amer. Soc. Hort. Sci.

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The catabolism of fatty acid (FA) is regarded as a key pathway of aroma volatile compounds in oriental sweet melon (Cucumis melo var. makuwa). In our research, two cultivars of oriental sweet melon, Caihong7 and Tianbao, were employed to illuminate which step of the biosynthetic pathway of aroma compounds could be regulated by ethylene (ETH). The role of ETH in determining the profiles of straight-chain aroma volatile compounds, levels of FA as aroma precursors, activities of aroma-related enzymes derived from FA pathway, and expression patterns of key enzymes were investigated. Overall, exogenous application of ETH increased the production rates of endogenous ETH and levels of FA. Compared with control, the level of straight-chain esters, especially the acetate, hexanoate, and hexyl esters, was significantly increased by ETH, whereas the content of alcohol and aldehyde reduced. In addition, the metabolism of free FA included linoleic acid (LA), linolenic acid (LeA), and oleic acid (OA) appeared to be ETH-dependent. The activities of lipoxygenase (LOX), alcohol dehydrogenase (ADH), and alcohol acetyltransferase (AAT) as well as the expression patterns of Cm-ADH1, Cm-ADH2, Cm-AAT1, and Cm-AAT4 were positively regulated by ETH. In contrast, hydroperoxide lyase (HPL) and Cm-AAT2 and Cm-AAT3 seemed to be independent of ETH modulation. These results suggested that the dissimilation of FA included LA, LeA, and OA into the acetate, hexanoate, and hexyl esters mainly through ETH regulating the LOX pathway by enhancing the expression of particular members of aroma-related key enzyme gene families as well as the activities of dehydrogenation and esterification.

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Section: Discussionsupporting

confidence: 64%

Role of Ethylene in the Biosynthetic Pathway of Related-aroma Volatiles Derived from Fatty Acids in Oriental Sweet Melon

Li¹,

Jin

et al. 2016

J. Amer. Soc. Hort. Sci.

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“…Esters are synthesized by AAT from the reactions of aliphatic and aromatic alcohols to their esters in the presence of acyl CoA in fruit and flowers (El-Sharkawy et al, 2005;Shan et al, 2012). In apple fruit, the levels of volatile production, AAT activity, and substrate were higher in peel than flesh.…”

Section: Introductionmentioning

confidence: 99%

Physiological, biochemical and sensory characterization of the response to waxing and storage of two mandarin varieties differing in postharvest ethanol accumulation

Ummarat

Arpaia

Obenland

2015

Postharvest Biology and Technology

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“…In earlier study (Shan et al, 2012), melons transformed with antisense AAT resulted in levels of mRNA transcripts and lower enzymatic activity than WT fruits. This caused a reduction in esters production.…”

Section: Antioxidant Assay Activitymentioning

confidence: 85%

The effect of ethylene on transgenic melon ripening and fruit quality

Lucchetta¹,

Rombaldi²,

Ja³

et al. 2014

Afr. J. Biotechnol.

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Antisense suppression of alcohol acetyltransferase gene in ripening melon fruit alters volatile composition

Cited by 25 publications

References 34 publications

Role of Ethylene in the Biosynthetic Pathway of Related-aroma Volatiles Derived from Fatty Acids in Oriental Sweet Melon

Role of Ethylene in the Biosynthetic Pathway of Related-aroma Volatiles Derived from Fatty Acids in Oriental Sweet Melon

Physiological, biochemical and sensory characterization of the response to waxing and storage of two mandarin varieties differing in postharvest ethanol accumulation

The effect of ethylene on transgenic melon ripening and fruit quality

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