Antiseptic effect of natural teat dip containing lactic acid against mastitis-causing Escherichia coli

Chotigarpa, Rinrada; Lampang, Kannika Na; Pikulkaew, Surachai; Okonogi, Siriporn; Silman, Pirote; Mektrirat, Raktham

doi:10.14202/vetworld.2019.397-401

Cited by 13 publications

(7 citation statements)

References 30 publications

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“…Interestingly, the physicochemical characteristics of the modified rice powders can be feasibly made into good gelling agents in pharmaceutical gel preparation [ 11 ]. Moreover, the successful development of a mucoadhesive buccal film and an antiseptic hydrogel from rice for pharmaceutical delivery systems were previously published [ 12 , 13 , 14 ]. Hence, in developing anthelminthic preparation loading, the PZQ into modified rice gel should have more advantages for biological and pharmaceutical aspects.…”

Section: Introductionmentioning

confidence: 99%

Preclinical Evaluations of Modified Rice Hydrogel for Topical Ophthalmic Drug Delivery of Praziquantel on Avian Philophalmiasis

et al. 2021

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The present study aims to evaluate the efficacy of a novel drug delivery system of the modified rice hydrogel containing praziquantel (PZQ) against Philophthalmus gralli isolated from ostrich eyes and determine the toxicity of the preparation on chicken eye model. The parasiticidal activity of PZQ (0, 1, 10, and 100 µg/mL) was tested on P. gralli. The ophthalmic antiparasitic hydrogel was formulated with appropriate amount of PZQ and chemically modified rice gel. The parasitic morphology after exposure with the preparation was examined under scanning electron microscope (SEM). The anthelminthic efficacy of the preparation on motility and mortality of parasites was performed by visual inspection and vital dye staining. The ocular irritation of the preparation was evaluated for 21 days using standard avian model followed by OECD 405. The results demonstrated that the parasiticidal activity of PZQ against P. gralli appears to be in a concentration- and time-dependent manner. In addition, the concentration of PZQ 10 µg/mL (Chi squared test, p = 0.003) and exposure time for 24 h (log-rank test, p = 0.0004) is sufficient to kill parasites, when statistically compared to negative control group. Rice hydrogel containing a lethal concentration of 10 µg/mL PZQ was successfully prepared. The preparation illustrated good parasitic killing and motile inhibiting effect on P. gralli compared with PZQ 10 µg/mL and its control (p < 0.05). An appearance under SEM of non-viable parasite after being incubated with the preparation, showing parasitic deformity, was observed comparing with the viable parasite in 0.9% normal saline solution (NSS). Moreover, no irritation of chicken eyes was also observed. Our results contribute to understanding the efficacy and the safety of the rice hydrogel of PZQ which have a predictive value for controlling P. gralli on the animal eyes. However, the pharmacological application needs to be further investigated for the best possible therapeutic approach.

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Section: Introductionmentioning

confidence: 99%

Preclinical Evaluations of Modified Rice Hydrogel for Topical Ophthalmic Drug Delivery of Praziquantel on Avian Philophalmiasis

et al. 2021

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“…A mulberry extract at 0.32 to 1.28 mg/mL (MIC and MBC) was shown to inhibit E. coli, S. aureus, and Salmonella typhi (Aelenei et al, 2019). A M. alba extract also inhibited the growth of S. aureus ATCC 33591 and ATCC 43300 at 250 µg/ mL (MIC or MBC) (Chotigarpa et al, 2019). In accordance with previous studies on the antibacterial properties of some organic acids (Pangprasit et al (2021) and herbal extract (Kuraeiad, et al, 2022) against reference and major mastitis pathogen strains of E. coli and S. aureus.…”

Section: Antibacterial Activity By Determination Of the Mic And Mbcmentioning

confidence: 99%

Antibacterial effect of ethanolic Morus alba Linn. leaf extract against mastitis-causing Escherichia coli and Staphylococcus aureus in vitro

Phengvongsone¹,

Thamrongyoswittayakul²,

Sukon³

et al. 2022

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This study was conducted to determine the in vitro antibacterial effect of an ethanolic Morus alba L. (mulberry) leaf extract against Escherichia coli and Staphylococcus aureus isolated from mastitis dairy cows and against E. coli ATCC 25922 and S. aureus ATCC 25923 reference strains. The antibacterial efficacy was evaluated by a microdilution method to determine the MIC, MBC, and a time-kill assay by using azithromycin as a positive control. The data were analyzed nonparametric tests with a significance level of p<0.05. The quercetin content of the mulberry leaf extract was 83.187 ± 0.272 µg/g dried plant material, as determined by HPLC. The extract had MICs of 10, 20, 10, and 10 mg/mL and MBCs of 20, 20, 20, and 10 mg/mL for E. coli ATCC 25922, E. coli ECCM62, S. aureus ATCC 25923, and S. aureus SACM62, respectively. Quercetin had relevant MIC of 10 mg/ml and MBC of 20 mg/mL to the extract for both ATCC strains. Time-kill tests showed a complete elimination of S. aureus ATCC 25923 and S. aureus SACM62 after a 30-min exposure to 10 and 20 mg/mL M. alba extract. E. coli ATCC 25922 and E. coli ECCM62 showed reductions of 6.8 log CFU/mL after a 360 min exposure to 40 mg/mL M. alba extract. In this study, the inhibitory effect was stronger against S. aureus than against E. coli (p<0.05). Overall, the M. alba L. extract showed appreciable in vitro antibacterial efficacy against mastitis-causing E. coli and S. aureus strains and against reference ATCC strains

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“…Additionally, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of pure lactic acid were checked for E. coli strains. The MIC and MBC were 0.5% lactic acid [ 110 , 111 ].…”

Section: Prevention Of Udder Infections In Cattle With Particular Emphasis On Bacterioci Like-substancementioning

confidence: 99%

Probiotics and Postbiotics as Substitutes of Antibiotics in Farm Animals: A Review

Zamojska

Nowak

et al. 2021

Animals

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Since 2006, the use of growth-promoting antibiotics has been banned throughout the European Union. To meet the expectations of livestock farmers, various studies have been carried out with the use of lactic acid bacteria. Scientists are trying to obtain the antimicrobial effect against the most common pathogens in large-scale farms. Supplementing the diet of broilers with probiotics (live, nonpathogenic microorganisms) stabilized the intestinal microbiota, which improved the results of body weight gain (BWG) and feed intake (FI). The positive effect of probiotics based on lactic acid bacteria has been shown to prevent the occurrence of diarrhea during piglet weaning. The antagonistic activity of postbiotics (inanimate bacteria, cell components, or post-fermentation by-products) from post-culture media after lactobacilli cultures has been proven on Staphylococcus aureus—the pathogen most often responsible for causing mastitis among dairy cows. The article aims to present the latest research examining the antagonistic effect of lactic acid bacteria on the most common pathogens in broilers, piglets, pigs, and cow farms.

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Antiseptic effect of natural teat dip containing lactic acid against mastitis-causing Escherichia coli

Cited by 13 publications

References 30 publications

Preclinical Evaluations of Modified Rice Hydrogel for Topical Ophthalmic Drug Delivery of Praziquantel on Avian Philophalmiasis

Preclinical Evaluations of Modified Rice Hydrogel for Topical Ophthalmic Drug Delivery of Praziquantel on Avian Philophalmiasis

Antibacterial effect of ethanolic Morus alba Linn. leaf extract against mastitis-causing Escherichia coli and Staphylococcus aureus in vitro

Probiotics and Postbiotics as Substitutes of Antibiotics in Farm Animals: A Review

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