Antisickling Activity and Thermostability of Anthocyanins Extract from a Congolese Plant, Hymenocardia acida Tul. (Hymenocardiaceae)

Mpiana, P. T.; Mudogo, Virima; Kabangu, Y. F.; Tshibangu, Damien S. T.; Ngbolua, Koto-te-Nyiwa; Atibu, Emmanuel K.; ., K.P. Mangwala; Mbala, M. B.; Makelele, L. K.; Bokota, M. T.

doi:10.3923/ijp.2009.65.70

Cited by 25 publications

(36 citation statements)

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“…A similar result was already obtained for some medicinal plant species used for the management of SCD by Congolese traditional healers. This activity could be due to compounds easily extracted by the used polar solvents such as anthocyanins or to phenolic or triterpenoic acids as previously reported [13][14][15][16][17][18][19][20]23]. The treated SS RBCs demonstrated a remarkable similarity to normal blood values.…”

Section: Extraction Yieldsmentioning

confidence: 82%

“…Fractions were filtered and concentrated to dryness under reduced pressure using a rotary evaporator. Extraction of anthocyanins was then done using 100 g of dried powdered plant material with acidified methanol (1% HCl) following an established protocol [13][14][15][16][17][18][19][20]. Anthocyanins extract was then defatted by n-hexane.…”

Section: Selection and Plant Materials Collectionmentioning

confidence: 99%

“…The first-line clinical management of SCD includes medullar transplantation, repeated blood transfusion to stabilize the patient's hemoglobin level, and the use of chemical agents which interfere with the mechanism and/or kinetics of the sickling process. Unfortunately, all current proposed therapies are quite expensive and have attendant risk factors in terms of clinical use [6][7][8]. Therefore, there is a need for more definite and effective treatments for the disease.…”

Section: Introductionmentioning

confidence: 99%

“…Herbal extracts have been used in African folk medicine for decade in the management of various ailments [9][10][11][12]. Recently our research team shows that many medicinal plants used in traditional medicine in DRC to manage SCD had in vitro antisickling activity and that this activity is mainly due to anthocyanins [13][14][15][16][17][18][19][20]. As several other flavonoids, anthocyanins are natural products with a range of biological activities including free radical scavengers and show antioxidant activity [21,22].…”

Section: Introductionmentioning

confidence: 99%

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Ethno-Pharmacological Survey, In Vitro Anti-Sickling and Free Radical Scavenging Activities of Carapa Procera DC. Stem Bark (Meliaceae)

Ngbolua¹,

Bishola²,

Mpiana³

et al. 2014

nova-jmbs

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Drepanocytosis is a genetic and neglected disease, endemic in negroids population. One of the main characteristics of this pathology is the production of a large amount of free radicals, leading to a severe oxidative stress and the consumption of NO by free oxygen radicals, and/or by cell-free plasma heme. The consequences of this defect are hemolytic anemia and tissue damage brought about by the blockage of blood vessels by the sickled cells. The present study evaluated the antisickling and radical scavenging activities of extracts from Carapa procera stem bark using Emmel's test and the DPPH assay. Carapa procera was selected through an ethnopharmacological. The results showed that methanolic, ethyl acetate and dichloromethane soluble fractions, anthocyanins and organic acids exhibited a significant antisickling as revealed by the observed normal biconcave form of sickle erythrocyte (normalization rate > 70%) in hypoxic conditions. Methanolic extract exhibited a good radical scavenging activity (ED50 = 1.698 ± 0.079 µg/mL). The chemical screening performed on the plant revealed the presence of anthocyanins and organic acids which were then extracted. Total anthocyanins and organic acids revealed interesting antisickling and antioxidant properties that could justify the integration of Carapa procera in Congolese pharmacopoeia for the management of sickle cell disease. Bioactive extracts from this plant species could increase nitric oxide by scavenging free oxygen radicals. For the best our knowledge, Carapa procera has not been yet previously reported as antisickling plant in the traditional medicine database of Democratic Republic of the Congo.

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Section: Extraction Yieldsmentioning

confidence: 82%

Section: Selection and Plant Materials Collectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Ethno-Pharmacological Survey, In Vitro Anti-Sickling and Free Radical Scavenging Activities of Carapa Procera DC. Stem Bark (Meliaceae)

Ngbolua¹,

Bishola²,

Mpiana³

et al. 2014

nova-jmbs

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“…f.) can be found wild or cultivated in Indonesia, India, China, Malaysia, Sri Lanka, the Philippines, and Bangladesh. The leaves of this plant have been reported for anti-angiogenic [1], antioxidant [2], antibactericidal [3], antifungal [4], anti-arthritic [5], anti-inflammatory and antinociceptive [6], antisickling [7], anthelmintic [8], cytotoxicity [9], larvicidal, and adulticidal activities [10]. In addition, its aerial parts showed in vitro HIV type 1 reverse transcriptase inhibitor [11], antiinflammatory and analgesic [12], sedative, and hypnotic activities [13].…”

Section: Introductionmentioning

confidence: 99%

Metabolite Profiling of Justicia gendarussa Burm. f. Leaves Using UPLC-UHR-QTOF-MS

Ningsih

2015

Sci. Pharm.

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An ultra-performance liquid chromatography ultra-high-resolution quadrupoletime-of-flight-mass spectrometry (UPLC-UHR-QTOF-MS) metabolite profiling of Justicia gendarussa Burm. f. leaves was performed. PCA and HCA analyses were applied to observe the clustering patterns and inter-sample relationships. It seemed that the concentrations of Ca, P, and Cu in the soil could affect the metabolite profiles of Justicia gendarussa. Six significant metabolites were proposed.

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Anti-sickling Activity of Ursolic Acid Isolated from the Leaves of Ocimum gratissimum L. (Lamiaceae)

Tshilanda

Onyamboko

Babady-Bila

et al. 2015

Nat. Prod. Bioprospect.

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The present study reports in vitro anti-sickling activity and phytochemical analyses of the leaves of Ocimum gratissimum. Biological testing revealed that the plant extracts possess antisickling effects. The combination of spectroscopic techniques: 1D-NMR, 2D-NMR and MS revealed that ursolic acid is the major biologically active compound of O. gratissimum (Silva et al. in Molecules 13:2482–2487, 2008; Kedar et al. J Food Drug Anal 20:865–871, 2012). This study is the first report of the antisickling activity of ursolic acid isolated from O. gratissimum. The pharmaceutical relevance of findings from this study derives from the possibility of integrating O. gratissimum as an antisickling plant in the pharmacopoeia of Democratic Republic of the Congo. The identification of the active principle could enhance the standardization of antisickling recipe.Graphical Abstract

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Antisickling Activity and Thermostability of Anthocyanins Extract from a Congolese Plant, Hymenocardia acida Tul. (Hymenocardiaceae)

Cited by 25 publications

References 0 publications

Ethno-Pharmacological Survey, In Vitro Anti-Sickling and Free Radical Scavenging Activities of Carapa Procera DC. Stem Bark (Meliaceae)

Ethno-Pharmacological Survey, In Vitro Anti-Sickling and Free Radical Scavenging Activities of Carapa Procera DC. Stem Bark (Meliaceae)

Metabolite Profiling of Justicia gendarussa Burm. f. Leaves Using UPLC-UHR-QTOF-MS

Anti-sickling Activity of Ursolic Acid Isolated from the Leaves of Ocimum gratissimum L. (Lamiaceae)

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