Antispasmodic activity of Warionia saharae Benthem ex Benth. &amp; Coss. on the rabbit and rat jejunums

Marghich, Mohamed; Makrane, Hanane; Alem, Chakib; Aziz, Mohammed

doi:10.56499/jppres21.1049_9.5.677

Cited by 5 publications

(6 citation statements)

References 25 publications

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“…reported the antispasmodic potential of Tylophora hirsuta in selected rabbit jejunum using a crude methanolic extract where a Ca ++ channel blockage activity was confirmed inhibiting diarrhoea. Our findings are also agreed with [42,39,43] who worked out on Warionia saharae , Thymus algeriensis and Fuchsia magellanica respectively strengthen our current findings.…”

Section: Resultssupporting

confidence: 93%

“…The standard protocol of [42] was used for the analysis of antispasmodic activity using charcoal movement test in the intestine. Albino mice were divided into 14 groups having 3 animals/group as following;…”

Section: Methodsmentioning

confidence: 99%

“…Ethyl acetate and methanolic extracts from the leaves and stem of R. afghanicum were studied for cytotoxic potential by using brine shrimp lethality bioassay following. [42] 20 mg of the extract were dissolved in 2 ml of the appropriate solvent, and 5, 50, and 500 ml of the solution were transferred to vials (3 vials/concentration), which represented 10, 100, and 1000 μg/ml, respectively. The solvent was allowed to evaporate for the whole night before adding 5 ml of seawater (36 g/L) to each vial.…”

Section: Cytotoxic Activitymentioning

confidence: 99%

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Pharmacological Activities of Rhododendron afghanicum; an Endemic Species of Khyber Pakhtunkhwa, Pakistan

Alam,

Ahmad,

Ahmad

et al. 2023

Chemistry & Biodiversity

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Majority of different kinds of metabolites having therapeutic characteristics are thought to be stored in medicinal plants. So, the present study was aimed to explore the crude extract of leaves and stem of R. afghanicum for phytochemical screening and various pharmacological activities. Toxicological studies at 100 mg/kg showed 60 % mortality where its safe dose level was 90 mg/kg. Phytochemical screening revealed the presence of alkaloids, glycosides, flavonoids and tannins in both extracts. Bacterial strains were susceptible to (RLEt) and (RLM) crude extracts except Staphylococcus aureus. RSM showed maximum anti‐inflammatory activity (20.16 %) followed by RSEt (20.14 %) where lowest activity was displayed by RLEt (18.46 %). Phytotoxic activity showed a substantial dose‐dependent phyto‐inhibition of Lemna minor. An outstanding cytotoxic potential was displayed with LD50 values of 9.46 and 13.03 μg/ml in both stem extracts. RLEt demonstrated a dose‐dependent pain relief at 30, 60 and 90 mg/kg which was 31 %, 40 % and 52 % respectively. A considerable spasmolytic action was observed by the shrinkage of jejunum muscle in albino mice. RLEt at 1000 ppm showed (17 mm) and RLM at 1000 ppm showed (16 mm) zone of inhibition against Aspergillus niger. These findings support and corroborate the traditional applications of R. afghanicum for treating digestive, analgesic and inflammatory ailments.

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Section: Resultssupporting

confidence: 93%

Section: Methodsmentioning

confidence: 99%

Section: Cytotoxic Activitymentioning

confidence: 99%

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Pharmacological Activities of Rhododendron afghanicum; an Endemic Species of Khyber Pakhtunkhwa, Pakistan

Alam,

Ahmad,

Ahmad

et al. 2023

Chemistry & Biodiversity

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“…It should also be mentioned that β-eudesmol, which is particularly abundant (15.8%) in our essential oil, stimulated small intestine motility and gastric emptying by inhibiting both the 5-HT 3 receptor and the dopamine D2 receptor [44]. To our knowl-edge, except our previous work conducted with the total aqueous extract [45], no study on the antispasmodic action of W. saharae essential oil has been done. Although the results of this study were comparable, far higher dosages (in mg of extract) were required to achieve comparable action, demonstrating that the essential oil fraction is certainly the most bioactive and allowing for a better understanding of the phytochemicals that may be responsible for this activity.…”

Section: Discussionmentioning

confidence: 93%

The Antispasmodic Effect of Warionia saharae Essential Oil in Experimental Models and its Mechanism of Action

Aziz¹,

Marghich²,

Addi³

et al. 2022

Front. Biosci. (Schol Ed)

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With several medicinal and aromatic species, the Asteraceae family is one of the largest angiosperm families. The genus Warionia is represented in this family by only one species, Warionia saharae. In Moroccan traditional medicine, this species is widely used to treat gastrointestinal problems. Essential oil of this plant (EoWs) was studied for possible myorelaxant and antispasmodic activities to rationalize some of the traditional uses. In this investigation, hydrodistillation was used to obtain the essential oil from the aerial part of the dry plant extract (EoWs), which was then analyzed using gas chromatography coupled to mass spectrometry (GC/MS). The major compounds identified in the EoWs are nerolidyl acetate (21.44%), β-Eudesmol (19.47%), linalool (16.48%), 1-terpinene-4-ol (10.93%), and cineole (5.34%). EoWs is relatively safe in the case of acute intake up to 2 g/kg body weight of albino mice. The effect of EoWs on intestinal relaxation was investigated using rabbit and rat jejunal smooth muscle. We have noticed that EoWs produce a myorelaxation on basal rabbit jejunum's contractions in a concentration-dependent manner with a maximal effect at 30 µg/mL. This myorelaxation was not dependent on adrenergic receptors. When the rat jejunums were pre-contracted with 25 mM KCl or 10 µM Carbachol (CCh), EoWs had an antispasmodic action with an IC50 values of 15.76 ± 0.37 and 12.04 ± 0.30 µg/mL, respectively. Preliminary results showed that it is probable that our plant might act directly through the NO and guanylate cyclase signaling pathway and on muscarinic but not nicotinic receptors. The results reveal that the Essential oil of W. saharae appears to have an impact on intestinal relaxation in vitro conditions. This finding lends credence to the traditional usage of this plant to treat intestinal disorders.

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“…Although the detailed mechanism of action is not known, a more detailed examination is needed to determine whether AEJO inhibits the muscarinic receptors. Other species, such as Nepeta cataria L., Satureja hortensis , Warionia saharae , Anthemis mauritiana , and Artemisia herba alba , have produced comparable results ( 27 , 28 , 29 , 30 , 31 ).…”

Section: Discussionmentioning

confidence: 99%

Antispasmodic and antidiarrheal effects of <i>Juniperus oxycedrus</i> L. on the jejunum in rodents

Amrani,

Karim,

Marghich

et al. 2024

J. Smooth Muscle Res.

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Functional bowel disorders (FBD) have a major potential to degrade the standards of public life. Juniperus oxycedrus L. ( J. oxycedrus ) (Cupressaceae) has been described as a plant used in traditional medicine as an antidiarrheal medication. The present study is the first to obtain information on the antispasmodic and antidiarrheic effects of J. oxycedrus aqueous extract through in vitro and in vivo studies. An aqueous extract of J. oxycedrus (AEJO) was extracted by decoctioning air-dried aerial sections of the plant. Antispasmodic activity was tested in an isolated jejunum segment of rats exposed to cumulative doses of drogue extract. The antidiarrheic activity was tested using diarrhea caused by castor oil, a transit study of the small intestine, and castor oil-induced enteropooling assays in mice. In the jejunum of rats, the AEJO (0.1, 0.3 and 1 mg/ml) diminished the maximum tone induced by low K + (25 mM), while it exhibited a weak inhibitory effect on high K + (75 mM) with an IC 50 =0.49 ± 0.01 mg/ml and IC 50 =2.65 ± 0.16 mg/ml, respectively. In the contractions induced by CCh (10 −6 M), AEJO diminished the maximum tone, similar to that induced by low K + (25 mM). with an IC 50 =0.45 ± 0.02 mg/ml. The inhibitory effect of AEJO on low K + induced contractions was significantly diminished in the presence of glibenclamide (GB) (0.3 µM) and 4-aminopyrimidine (4-AP) (100 µM), with IC 50 values of 1.84 ± 0.09 mg/ml. and 1.63 ± 0.16 mg/ml, respectively). The demonstrated inhibitory effect was similar to that produced by a non-competitive antagonist acting on cholinergic receptors and calcium channels. In castor oil-induced diarrhea in mice, AEJO (100, 200, and 400 mg/kg) caused an extension of the latency time, a reduced defecation frequency, and a decrease in the amount of wet feces compared to the untreated group (distilled water). Moreover, it showed a significant anti-motility effect and reduced the amount of fluid accumulated in the intestinal lumen at all tested doses. These findings support the conventional use of Juniperus oxycedrus L. as a remedy for gastrointestinal diseases.

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Antispasmodic activity of Warionia saharae Benthem ex Benth. & Coss. on the rabbit and rat jejunums

Cited by 5 publications

References 25 publications

Pharmacological Activities of Rhododendron afghanicum; an Endemic Species of Khyber Pakhtunkhwa, Pakistan

Pharmacological Activities of Rhododendron afghanicum; an Endemic Species of Khyber Pakhtunkhwa, Pakistan

The Antispasmodic Effect of Warionia saharae Essential Oil in Experimental Models and its Mechanism of Action

Antispasmodic and antidiarrheal effects of <i>Juniperus oxycedrus</i> L. on the jejunum in rodents

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