Antispasmodic and Myorelaxant Activity of Organic Fractions from Origanum majorana L. on Intestinal Smooth Muscle of Rodents

Makrane, Hanane; Aziz, Mohammed; Mekhfi, Hassane; Ziyyat, Abderrahim; Bnouham, Mohamed; Legssyer, Abdelkhaleq; Gressier, Bernard; Eto, Bruno

doi:10.9734/ejmp/2018/41075

Cited by 14 publications

(9 citation statements)

References 27 publications

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“…The method described by Makrane et al 10 was used. After having anesthetized the animal, the abdominal cavity was opened, and 2 cm jejunum was removed and preserved during the tests in the oxygenated standard Krebs-Henseleit buffer (KHB) solution with the following composition (in mM): NaCl 118, KCl 4.7, CaCl2 2.5, MgSO4 1.2, NaHCO3 25, KH2PO4 1.2 and glucose 10.…”

Section: In Vitro Experimentsmentioning

confidence: 99%

Antidiarrheal Activity of Aqueous Extract of Artemisia campestris L. subsp. Glutinosa

2021

TJNPR

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Artemisia campestris L. subsp. glutinosa is used as a remedy for diarrhea in Moroccan folk medicine but has not been investigated for its antidiarrheal property. The aim of the present study was to evaluate the antidiarrheal activity of the aqueous extract of this plant, in order to verify it scientifically. The extract was prepared by infusion of the aerial part of the plant. The antidiarrheal activity was investigated using two standard methods, castor oil-induced diarrhoea, and gastrointestinal motility in mice. In addition, the antispasmodic activity was determined in vitro on the isolated rat jejunum mounted in an isotonic transducer. The extract caused a dosedependent relaxation of the jejunum tone of rat pre-contracted by KCl (25 mM) with an IC50 of 1.20 ± 0.20 mg/mL. Oral pretreatment of mice with the extract at 100, 200, and 400 mg/kg,bw, delayed the onset of diarrhea, reduced the total number of defecations during 4 h, and decreased the total number of wet faeces in 4 h by 5.08, 18.64, and 38.98%, respectively. The results suggest that A. campestris L. has antidiarrheal activity and confirms its traditional use.

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Section: In Vitro Experimentsmentioning

confidence: 99%

Antidiarrheal Activity of Aqueous Extract of Artemisia campestris L. subsp. Glutinosa

2021

TJNPR

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“…This hypothesis was confirmed when the extract shifted the carbachol dose-response curve to the right and down, inhibiting the contractile response of intestinal smooth muscle at increasing doses of carbachol. Therefore, this inhibitory effect is similar to that of a non-competitive antagonist to the cholinergic receptors (19). The antagonist binds to the receptor at a site separate from the agonist-binding site (allosteric site) and results in conformational changes in the receptor with a decreased affinity of the receptor for its agonist.…”

Section: These Waves Of Depolarization Activate Voltage-dependent Calcium Channels In the Smooth Muscle Cells Andmentioning

confidence: 84%

“…According to Godfraind et al (33), any substance that prevents these contractions (induced by the KCl rich medium) is considered to be an inhibitor of voltage-gated calcium channels. This hypothesis was reinforced by the fact that the extract at 0.1, 0.3, and 1 mg/ml shifted the dose-response curve of CaCl 2 to the right and down by inhibiting the response to increasing doses of calcium, therefore, this inhibitory effect is similar to that of a non-competitive antagonist to the voltage-dependent calcium channels (19). These findings are reinforced when we used verapamil, which is an antagonist of L-type voltage calcium channel blocker, which had an effect comparable to that of 1 mg/ml of ACAE.…”

Section: These Waves Of Depolarization Activate Voltage-dependent Calcium Channels In the Smooth Muscle Cells Andmentioning

confidence: 99%

Myorelaxant and antispasmodic effect of an aqueous extract of <i>Artemisia campestris</i> L. via calcium channel blocking and anticholinergic pathways

Marghich

Aziz

Mekhfi

et al. 2021

J. Smooth Muscle Res.

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Intestinal spasms are violent contractions that occur in the intestine, which cause discomfort to people who have them. Medicinal plants are widely used in traditional Moroccan medicine to treat these problems, among these being Artemisia campestris L. This study aims to evaluate the relaxant and antispasmodic effects of an aqueous extract of this plant (ACAE). It was performed in vitro on isolated segments of both isolated rat and rabbit jejunum mounted in an organ bath and tension recordings made via an isotonic transducer. ACAE caused a myorelaxant effect on baseline rabbit jejunum contractions in a dose-dependent and reversible manner with an IC 50 of 1.52 ± 0.12 mg/ml. This extract would not act via adrenergic receptors pathway. On the other hand, the extract caused a dose-dependent relaxation of the jejunum tone in rat jejenum segments pre-contracted with either Carbachol (CCh; 10 −6 M) or high K + (KCl 75 mM) with an IC 50 = 0.49 ± 0.02 mg/ml and 0.36 ± 0.02 mg/ml respectively. In the presence of different doses of the extract, the maximum response to CCh and CaCl 2 was significantly reduced. This demonstrates that ACAE acts on both muscarinic receptors and voltage-dependent calcium channels. Thus, the plant extract acted on both muscarinic and nicotinic receptors and acts on the guanylate cyclase pathway, but not the nitric oxide pathway. These results indicate the mechanism by which Artemisia campestris L. acts as an effective antispasmodic agent in traditional Moroccan medicine.

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“…Some of these studies presented detailed mechanistic explanations of the antispasmodic effect for the non-fractioned essential oils. Makrane et al [37] showed that organic fractions rich in essential oils from Origanum majorana showed a consistent spasmolytic effect on isolated rat and rabbit ileum not altered by atropine, L-NAME or methylene blue, and shifted to the right the concentrations-response curves for CaCl 2 , suggesting a calcium channel blocking effect. Rasheed et al [45] showed in an experiment on isolated rabbit jejunum that essential oil from Rosa indica relaxed the organ, shifting the calcium curves to the right, showing a similar effect to verapamil, a phenylalkylamine derivative from the calcium channel blocker class.…”

Section: Resultsmentioning

confidence: 99%

Antispasmodic Effect of Essential Oils and Their Constituents: A Review

et al. 2019

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The antispasmodic effect of drugs is used for the symptomatic treatment of cramping and discomfort affecting smooth muscles from the gastrointestinal, billiary or genitourinary tract in a variety of clinical situations.The existing synthetic antispasmodic drugs may cause a series of unpleasant side effects, and therefore the discovery of new molecules of natural origin is an important goal for the pharmaceutical industry. This review describes a series of recent studies investigating the antispasmodic effect of essential oils from 39 plant species belonging to 12 families. The pharmacological models used in the studies together with the mechanistic discussions and the chemical composition of the essential oils are also detailed. The data clearly demonstrate the antispasmodic effect of the essential oils from the aromatic plant species studied. Further research is needed in order to ascertain the therapeutic importance of these findings.

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Antispasmodic and Myorelaxant Activity of Organic Fractions from Origanum majorana L. on Intestinal Smooth Muscle of Rodents

Cited by 14 publications

References 27 publications

Antidiarrheal Activity of Aqueous Extract of Artemisia campestris L. subsp. Glutinosa

Antidiarrheal Activity of Aqueous Extract of Artemisia campestris L. subsp. Glutinosa

Myorelaxant and antispasmodic effect of an aqueous extract of <i>Artemisia campestris</i> L. via calcium channel blocking and anticholinergic pathways

Antispasmodic Effect of Essential Oils and Their Constituents: A Review

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