Antithrombin III prevents 60 min warm intestinal ischemia reperfusion injury in rats

Özden, Akın; Tetik, Cihat; Bi̇lgi̇han, Ayşe; Çallı, Neşe; Bostancı, Birol; Yis, Özgür Mehmet; Düzcan, Ender

doi:10.1007/s004330050107

Cited by 53 publications

(53 citation statements)

References 25 publications

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“…This is consistent with previous reports that the peroxidation of cellular membrane lipids and oxidation of cellular proteins, determined by MDA and PCC, respectively, increases by ROS/RNS following I/R injury. [3,8,[30][31][32][33] In addition, we found that the oral supplementation of rats with PA prior to I/R significantly reduced the extent of lipid peroxidation and protein oxidation in the kidneys, indicating decreased cellular injury. Protection of cells might be the consequence of molecular structure of PA, which allows them to accumulate at lipid interfaces [28] and/or to penetrate into membranes.…”

Section: Discussionmentioning

confidence: 66%

Ameliorative Effects of Proanthocyanidin on Renal Ischemia/Reperfusion Injury

et al. 2008

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NTRODUCTION Several natural products have been reported to have beneficial effects on ischemia/reperfusion (I/R) injury, particularly from a preventative perspective. Therefore, this study was designed to investigate the efficiency of proanthocyanidin (PA), a natural product derived from grape seed, on renal dysfunction and injury induced by I/R of rat kidney. MATERIALS AND METHODS Twenty‐four male Sprague‐Dawley rats were divided into three groups: sham‐operated, I/R, I/R+PA. Rats were given PA (100 mg/kg/day peroral) 7 days prior to I/R. All rats except sham‐operated underwent 60 min of bilateral renal ischemia followed by 6 h of reperfusion. After reperfusion, kidneys and blood were obtained for evaluation. Superoxide dismutase, glutathione peroxidase, malondialdehyde, protein carbonyl content, and nitrite/nitrate level (NO(x)) were determined in the renal tissue. Serum creatinine (S(Cr)), blood urea nitrogen (BUN), and aspartate aminotransferase (AST) were determined in the blood. Additionally, renal sections were used for histological grade of renal injury. RESULTS PA significantly reduced the I/R‐induced increases in S(Cr), BUN, and AST. In addition, PA markedly reduced elevated oxidative stress product, restored decreased antioxidant enzymes, and attenuated histological alterations. Moreover, PA attenuated the tissue NO(x), levels indicating reduced NO production. CONCLUSIONS The pretreatment of rats with PA reduced the renal dysfunction and morphological changes, ameliorated cellular injury, and restored renal antioxidant enzymes caused by renal I/R.

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Section: Discussionmentioning

confidence: 66%

Ameliorative Effects of Proanthocyanidin on Renal Ischemia/Reperfusion Injury

et al. 2008

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“…37 Myeloperoxidase activity in intestinal tissue increased 3-fold after 60-minute intestinal ischemia and 8-to 9-fold after 60-minute reperfusion. 38 Ozden and associates 27 showed that mucosal myeloperoxidase activity was increased in the control group, confirming postreperfusion neutrophil infiltration compared with that shown in the sham group. Reduced myeloperoxidase activity in the antithrombin groups showed that antithrombin inhibited polymorphonuclear infiltration in the reperfused intestine.…”

Section: Discussionmentioning

confidence: 70%

“…In addition, myeloperoxidase as an index of polymorphonuclear accumulation was significantly less in the IPC groups and especially in the groups treated with AT, in agreement with similar studies. 27 Tumor necrosis factor α is the central component of the proinflammatory cytokine cascade in liver IRI and is a crucial effecter of remote organ damage after hepatic IRI. 28,29 It has been reported that TNF-α elevation was responsible for pulmonary damage after hepatic IRI.…”

Section: Discussionmentioning

confidence: 99%

“…Antithrombin has shown promise in recent years and has been used in numerous studies in an attempt to improve organ outcomes after IRI. [12][13][14][15][16] Although both ischemic preconditioning (IPC) and antithrombin are well known individually, their potential for acting synergistically has not yet been studied. The murine model of IRI used in this study was designed specifically to answer the following 2 questions.…”

Section: Introductionmentioning

confidence: 99%

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Vrakas

Tsalis²,

Roidos³

et al. 2017

Exp Clin Transplant

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Objectives: Our study aimed to determine whether antithrombin plays a synergistic role in accentuating the effects of intestinal ischemic preconditioning. Materials and Methods: Fifty rats were randomly allocated to 5 groups (10 rats/group) as follows: sham treatment (group 1); ischemia-reperfusion (group 2); ischemic preconditioning followed by ischemiareperfusion (group 3); antithrombin + ischemia-reperfusion, similar to group 2 but including antithrombin administration (group 4); and antithrombin + ischemic preconditioning, similar to group 3 but including antithrombin administration (group 5). Blood samples and liver specimens were obtained for measurement of cytokines, myeloperoxidase, and malondialdehyde. Liver biopsies were examined by electron microscopy. Results: Intestinal ischemia-reperfusion induced a remote hepatic inflammatory response as evidenced by the striking increase of proinflammatory cytokines, myeloperoxidase, and malondialdehyde. Tumor necro sis factor-α levels in group 5 (12.48 ± 0.7 pg/mL) were significantly lower than in group 3 (13.64 ± 0.78 pg/mL; P = .014). Mean interleukin 1β was lower in group 5 (9.52 ± 0.67pg/mL) than in group 3 (11.05 ± 1.9 pg/mL; P > .99). Mean interleukin 6 was also significantly lower in group 5 (17.13 ± 0.54 pg/mL) than in group 3 (23.82 ± 1 pg/mL; P < .001). Myeloperoxidase levels were significantly higher in group 3 (20.52 ± 2.26 U/g) than in group 5 (18.59 ± 1.03 U/g; P = .025). However, malondialdehyde levels did not significantly improve in group 5 (4.55 ± 0.46 μmol) versus group 3 (5.17 ± 0.61 μmol; P = .286). Tumor necrosis factor-α, interleukin 6, and myeloperoxidase findings show that antithrombin administration further attenuated the inflammatory response caused by ischemia-reperfusion, suggesting a synergistic effect with ischemic preconditioning. These findings were confirmed by electron microscopy. Conclusions: The addition of antithrombin to ischemic preconditioning may act to attenuate or prevent damage from ischemia-reperfusion injury by inhi biting the release of cytokines and neutrophil infiltration.

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“…Using an Albino rat model, Herek infused the animals with phAT with 250 U/kg phAT prior to infecting them and creating a burned surface; phAT-treated rats had reduced intestinal villi degeneration and decreased bacterial translocation to mesenteric lymph nodes, spleen and liver compared to sham and control rat (P < 0.02) [62] . This study followed the work of Ozden et al [63] who showed that an infusion of phAT prevented an ischemic reperfusion injury in the rat.…”

Section: Bacterial Translocationmentioning

confidence: 99%

Antithrombin in the treatment of burn trauma

Kowal-Vern

Orkin

2016

WJCCM

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Antithrombin (AT) is a natural anticoagulant with antiinflammatory properties that has demonstrated value in sepsis, disseminated intravascular coagulation and in burn and inhalation injury. With high doses, AT may decrease blood loss during eschar excision, reducing blood transfusion requirements. There are no human randomized, placebo-controlled studies, which have tested the true benefit of this agent in these conditions. Two main forms of AT are either plasma-derived AT (phAT) and recombinant AT (rhAT). Major ovine studies in burn and smoke inhalation injury have utilized rhAT. There have been no studies which have either translated the basic rhAT research in burn trauma, or determined the tolerance and pharmacokinetics of rhAT concentrate infusions in burn patients. Advantages of rhAT infusions are no risk of blood borne diseases and lower cost. However, the majority of human burn patient studies have been conducted utilizing phAT. Recent Japanese clinical trials have started using phAT in abdominal sepsis successfully. This review examines the properties of both phAT and rhAT, and analyzes studies in which they have been utilized. We believe that it is time to embark on a randomized placebo-controlled multi-center trial to establish the role of AT in both civilian and military patients with burn trauma.

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Antithrombin III prevents 60 min warm intestinal ischemia reperfusion injury in rats

Cited by 53 publications

References 25 publications

Ameliorative Effects of Proanthocyanidin on Renal Ischemia/Reperfusion Injury

Ameliorative Effects of Proanthocyanidin on Renal Ischemia/Reperfusion Injury

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Antithrombin in the treatment of burn trauma

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