Antithrombotic and cytotoxic activities of four Bangladeshi plants and PASS prediction of their isolated compounds

Kabir, Mohammad Shah Hafez; Mahamoud, Md. Sofi; Chakrabarty, Nishan; Ahmad, Shabbir; Masum, Md. Abdullah Al; Hoque, Md. Akramul; Hossain, Mohammed Kamal; Rahman, Md. Mominur; Nasiruddin, Munira

doi:10.1515/jbcpp-2015-0144

Cited by 7 publications

(3 citation statements)

References 33 publications

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“…In vitro thrombolytic activity was performed according to the method described previously [ 34 ]. In the previously weighed Eppendorf tubes, 500 μL of human blood was transferred.…”

Section: Methodsmentioning

confidence: 99%

A comprehensive multi-directional exploration of phytochemicals and bioactivities of flower extracts from Delonix regia (Bojer ex Hook.) Raf., Cassia fistula L. and Lagerstroemia speciosa L.

Rahman

Ahmed

Noor

et al. 2020

Biochemistry and Biophysics Reports

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Delonix regia (Bojer ex Hook.) Raf., Cassia fistula L. and Lagerstroemia speciosa L. are three ornamental plants that produce colorful flowers. The present study aimed to evaluate the phytochemicals and bioactivities of methanolic extracts of flowers from Delonix regia (DrFME), Cassia fistula (CfFME), and Lagerstroemia speciosa (LsFME). The presence of ten different chemical classes in varying degrees was confirmed while qualitatively screened. During quantitative determination, LsFME possesses the highest amount of total phenolic (418.0 mg/g), flavonoid (50.8 mg/g), and tannin (256.3 mg/g) contents. The extracts showed excellent antioxidant capacity in a concentration-dependent manner with the lowest IC50 value (41.51 μg/mL) displayed by LsFME. LsFME paralyzed the experimental worms at 2.95 min and killed at 3.96 min. DrFME was found to be more effective in thrombolytic (35.5% clot lysis) and anticoagulant activities. Negligible hemolytic activity (IC50 > 200 μg/mL) found for all extracts which suggest their less potential toxicity. The in vivo experiments revealed that the CfFME has the highest analgesic (64.34% pain inhibition) activity while LsFME has the highest antidiarrheal (70.27% inhibition) and antihyperglycemic (46.94% inhibition) activities at 400 mg/kg of body weight doses. This study has shown the presence of phytochemicals and potential bioactivities which indicates the possibility of these flowers to be used as a source of phytochemicals as well as safe and effective natural medicine.

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“…In vitro thrombolytic activity was performed according to the method described previously [ 34 ]. In the previously weighed Eppendorf tubes, 500 μL of human blood was transferred.…”

Section: Methodsmentioning

confidence: 99%

A comprehensive multi-directional exploration of phytochemicals and bioactivities of flower extracts from Delonix regia (Bojer ex Hook.) Raf., Cassia fistula L. and Lagerstroemia speciosa L.

Rahman

Ahmed

Noor

et al. 2020

Biochemistry and Biophysics Reports

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“…Numerous reports devoted to pro-fibrinolytic or thrombolytic activity of different plant extracts or isolated compounds have been published so far [ 106 , 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 , 115 , 116 , 117 , 118 , 119 , 120 , 121 ]. Using plants as sources of fibrinolytic substances seems to be an attractive prospect for future research, but as yet, most of the available data derive from basic in vitro screening tests employing whole blood or plasma fibrin clots ( Table 5 ).…”

Section: Pro-fibrinolytic Effects Of Natural Plant-derived Substancesmentioning

confidence: 99%

Plant-Derived Compounds and Extracts as Modulators of Plasmin Activity—A Review

Kołodziejczyk-Czepas

Czepas

2023

Molecules

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Functionality of the fibrinolytic system is based on activity of its central enzyme, plasmin, responsible for the removal of fibrin clots. Besides the hemostasis, fibrinolytic proteins are also involved in many other physiological and pathological processes, including immune response, extracellular matrix degradation, cell migration, and tissue remodeling. Both the impaired and enhanced activity of fibrinolytic proteins may result in serious physiological consequences: prothrombotic state or excessive bleeding, respectively. However, current medicine offers very few options for treating fibrinolytic disorders, particularly in the case of plasmin inhibition. Although numerous attempts have been undertaken to identify natural or to develop engineered fibrinolytic system modulators, structural similarities within serine proteases of the hemostatic system and pleiotropic activity of fibrinolytic proteins constitute a serious problem in discovering anti- or profibrinolytic agents that could precisely affect the target molecules and reduce the risk of side effects. Therefore, this review aims to present a current knowledge of various classes of natural inhibitors and stimulators of the fibrinolytic system being well-defined low-molecular plant secondary metabolites or constituents of plant extracts as well as plant peptides. This work also discusses obstacles caused by low specificity of most of natural compounds and, hence, outlines recent trends in studies aimed at finding more efficient modulators of plasmin activity, including investigation of modifications of natural pharmacophore templates.

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“…Whole venous blood (5 mL) was drawn from healthy volunteers (n= 10) without a history of oral contraception or anticoagulant therapy. An informed consent form was filled up by every subject participating willingly in this research project [34]. A lab technician from the Department of Pharmacy, University of Chittagong, who collected the blood from the volunteers, preserved the blood by the same lab personnel.…”

Section: Blood Specimenmentioning

confidence: 99%

Assessment of the Pharmacological Activities of Ardisia solanacea Roxb: An Ethnomedicinal Plant used in Bangladesh

Islam

Proma

Naima

et al. 2020

J. Pharm. Nutr. Sci.

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Objective: This study aims to uncover the anti-diarrheal, antioxidant, thrombolytic, and anthelmintic activities of methanol extract of A. solanacea (ASME) and its soluble n-hexane fraction in methanol (ASNH).Materials and Methods: The phytochemical assessment of this plant was performed by using the standard method. The anti-diarrheal property was screened by castor oil induced diarrhea in Swiss albino mice and plant extract was administered into mice by oral gavage. The antioxidant property was being investigated by two different in vitro methods such as ferric reducing effect assay and superoxide scavenging activity assay. The thrombolytic activity was evaluated by in vitro clot lysis procedure, and the anthelmintic study was carried out on earthworm Pheretima posthuma.Results: In castor-oil induced diarrhea, ASME and ASNH induced a significant decrease (**P

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Antithrombotic and cytotoxic activities of four Bangladeshi plants and PASS prediction of their isolated compounds

Abstract: Through our study it was found that A. bulbifer and P. sikkimensis could be considered as very promising and beneficial thrombolytic agents.

Cited by 7 publications

References 33 publications

A comprehensive multi-directional exploration of phytochemicals and bioactivities of flower extracts from Delonix regia (Bojer ex Hook.) Raf., Cassia fistula L. and Lagerstroemia speciosa L.

A comprehensive multi-directional exploration of phytochemicals and bioactivities of flower extracts from Delonix regia (Bojer ex Hook.) Raf., Cassia fistula L. and Lagerstroemia speciosa L.

Plant-Derived Compounds and Extracts as Modulators of Plasmin Activity—A Review

Assessment of the Pharmacological Activities of Ardisia solanacea Roxb: An Ethnomedicinal Plant used in Bangladesh

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