Antitumor Activities of an Anthraquinone Fraction Isolated From In Vitro Cultures of <i>Ophiorrhiza rugosa</i> var <i>decumbens</i>

Raveendran, Vineesh Vimala; Vijayan, Fijesh P; Padikkala, José

doi:10.1177/1534735411403478

Cited by 6 publications

(4 citation statements)

References 31 publications

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“…Ophiorrhiza pumila is a dicotyledonous plant classified into Rubiaceae family and remains as a sustainable source of camptothecin (CPT). CPT is a type of monoterpene indole alkaloids (MIAs) commonly used in treatment of cancers and was initially isolated from Camptotheca acuminate [ 1 ], and subsequently detected in Nothapodytes nimmoniana and other plants [ 2 – 4 ]. CPT inhibits tumor growth by blocking DNA topoisomerase I [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide survey of the GATA gene family in camptothecin-producing plant Ophiorrhiza pumila

et al. 2022

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Background Ophiorrhiza pumila (Rubiaceae) is capable of producing camptothecin (CPT), one monoterpene indole alkaloid extensively employed in the treatment of multiple cancers. Transcription factors (TFs) GATA are a group of transcription regulators involved in plant development and metabolism, and show the feature of binding to the GATA motif within the promoters of target genes. However, GATA TFs have not been characterized in O. pumila. Result In this study, a total of 18 GATA genes classified into four subfamilies were identified, which randomly distributed on 11 chromosomes of O. pumila. Synteny analysis of GATA genes between O. pumila and other plant species such as Arabidopsis thaliana, Oryza sativa, Glycine max, Solanum lycopersicum, Vitis vinifera, and Catharanthus roseus genomes were analyzed. Tissue expression pattern revealed that OpGATA1 and OpGATA18 were found to be correlated with ASA, MK, CPR and GPPS, which were highly expressed in leaves. OpGATA7, showed high expression in roots as most of the CPT biosynthetic pathway genes did, suggesting that these OpGATAs may be potential candidates regulating CPT biosynthesis in O. pumila. Conclusions In this study, we systematically analyzed the OpGATA TFs, and provided insights into the involvement of OpGATA TFs from O. pumila in CPT biosynthesis.

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Section: Introductionmentioning

confidence: 99%

Genome-wide survey of the GATA gene family in camptothecin-producing plant Ophiorrhiza pumila

et al. 2022

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“…This is an essential outcome as the free reactive atoms or molecules are harmful due to their ability to damage normal and key biological macromolecules in the body, such as lipid, protein, and DNA. In contrast, the anthraquinone fraction of O. rugosa van decumbens functions by causing cytotoxicity in cancer cells and increasing the intracellular ROS, thus leading to cell apoptosis [67].…”

Section: Anticancer Activitymentioning

confidence: 99%

“…To date, no exact mechanism of action for the anti-inflammatory activity of anthraquinone fraction from O. rugosa has been underlined. However, it is believed that inflammation reduction occurs via the inhibition of the lipid peroxidation and scavenging of hydroxyl radicals [67]. Table 5 shows the anticancer activities of certain Ophiorrhiza species based on previous studies.…”

Section: Anticancer Activitymentioning

confidence: 99%

“…For example, undifferentiated callus and suspension cultures do not always produce the desired amount of compound of interest. In contrast, the shoot, root, and hairy root cultures will often generate the same compounds as in the appropriate organs [67]. Hence, the type of medium culture, suitable salt strength, nitrate level, phosphate level, and growth regulator level should be considered beforehand to obtain the desired metabolites at a high quantity and quality [84].…”

Section: Propagationmentioning

confidence: 99%

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Genus Ophiorrhiza: A Review of Its Distribution, Traditional Uses, Phytochemistry, Biological Activities and Propagation

et al. 2020

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Almost 50 species of Ophiorrhiza plants were reviewed in this work and the main objective is to critically analyse their distribution, phytochemical content, biological activity, and propagation. Moreover, the information would be useful in promoting the relevant uses of the plant, especially in the medicinal fields based on in vitro and in vivo studies. To this end, scientific sources, including theses, PubMed, Google Scholar, International Islamic University Malaysia IIUM EBSCO, PubChem, and Elsevier, were accessed for publications regarding the Ophiorrhiza genus in this review. Scientific literature regarding the Ophiorrhiza plants revealed their wide distribution across Asia and the neighbouring countries, whereby they were utilised as traditional medicine to treat various diseases. In particular, various active compounds, such as alkaloids, flavonoids, and terpenoids, were reported in the plant. Furthermore, the Ophiorrhiza species showed highly diverse biological activities, such as anti-cancer, antiviral, antimicrobial, and more. The genus propagation reported could produce a high quality and quantity of potent anticancer compound, namely camptothecin (CPT). Hence, it is believed that the relevant uses of natural compounds present in the plants can replace the existing crop of synthetic anticancer drugs associated with a multitude of unbearable side effects. Additionally, more future studies on the Ophiorrhiza species should be undertaken to establish the links between its traditional uses, active compounds, and pharmacological activities reported.

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Cytotoxic and anti-inflammatory activities of secondary metabolites from Ophiorrhiza baviensis growing in Thua Thien Hue, Vietnam

Cuong

Anh

Dat

et al. 2019

Natural Product Research

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A new ursane-type triterpene, 3β,23,24-trihydroxyurs-12-en-28-oic acid (1), together with eight known compounds (2-9) were isolated from the aerial parts of Ophiorrhiza baviensis. Among them, compounds 2-5 were found for the first time from the genus Ophiorrhiza, while compounds 6-9 were reported from O. baviensis for the first time. Their structures were elucidated by analysis of HR-ESI-MS and NMR ( 1 H-NMR, 13 C-NMR, HSQC, and HMBC) spectra, as well as comparison with those reported in the literature. Moreover, all isolated compounds were evaluated for their cytotoxic activities against MCF-7, Hela, KB, A549, and SK-LU-1 cancer cell lines and their effects on LPSinduced NO production in RAW264.7 cells. This is the first report of chemical constituents and biological activities of O. baviensis.

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Antitumor Activities of an Anthraquinone Fraction Isolated From In Vitro Cultures of Ophiorrhiza rugosa var decumbens

Cited by 6 publications

References 31 publications

Genome-wide survey of the GATA gene family in camptothecin-producing plant Ophiorrhiza pumila

Genome-wide survey of the GATA gene family in camptothecin-producing plant Ophiorrhiza pumila

Genus Ophiorrhiza: A Review of Its Distribution, Traditional Uses, Phytochemistry, Biological Activities and Propagation

Cytotoxic and anti-inflammatory activities of secondary metabolites from Ophiorrhiza baviensis growing in Thua Thien Hue, Vietnam

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