Antitumor Activities of Iodoacetate and Dimethylsulphoxide Against Solid Ehrlich Carcinoma Growth in Mice

Abstract

Treatment of tumor-bearing mice with LD 12.5 values of iodoacetate; IAA (1.84 mg/100g b.w.) and/or dimethylsulphoxide; DMSO (350 mg/ 100g b.w.) significantly increased the cumulative mean survival time and percentage of survivors and reduced the mean tumor weight, compared to tumor-bearing controls, however, a more pronounced effect is recorded in the combined treatment. Also, an increase in the life span (ILS%) and tumor growth inhibition ratio (T/C%) are reported and amounted to 145.78 and 43.80%, 195.54 and 61.30% and 220.77 and 78.40% in IAA, DMSO and combined-treated groups, respectively. Results obtained from biochemical studies reveal that a single IAA treatment of tumor-bearing mice significantly increased the levels of plasma lactate dehydrogenase (LDH) activity, while it also significantly decreased the levels of plasma glucose and liver total protein, RNA and DNA, compared to normal controls. On the other hand, a single DMSO treatment significantly elevated the activities of blood antioxidant enzymes, i.e. glutathione peroxidase (GP x ) and glucose-6-phosphate dehydrogenase (G6PDH) and decreased the liver RNA and DNA levels. Combined treatment increased significantly the levels of plasma LDH and erythrocytes G6PDH activities, as well as liver glycogen, and in contrast it decreased the levels of liver total protein, RNA and DNA, compared to normal controls.

Effect of metformin and adriamycin on transplantable tumor model

“…These results were in the same line with Fahim et al (2003). This was attributed to the effects of Ehrlich tumor on the body with alteration of metabolism, decreased immunity, severe anemia and induction of cachexia (Kumar et al, 2011).…”

Highly Expressed Genes in Rapidly Proliferating Tumor Cells as New Targets for Colorectal Cancer Treatment

“…We show here that inhibition of GAPDH efficiently reduces the growth of colon cancer cells in vitro. Interestingly, although the GAPDH inhibitor Na iodoacetate has been shown to reduce the growth of Ehrlich ascites carcinoma (EAC) cells and xenografts of colon cancer cells at doses similar or lower than the one used in vivo in this study (35,36), no significant effects were observed here on the growth of subcutaneous xenografts of four different colon cancer cell lines. However, no toxicity was observed at the doses used, and based on the in vitro effects observed, it remains possible that Na iodoacetate treatment at higher doses and/or in other tumor cell lines, may interfere with tumor growth.…”

Modification of Gamma Radiation and 4-Nitroquinoline 1-Oxide Induced Genotoxicity by Tumour Promoter Iodoacetate

“…If inhibition in energy production reported above by others was responsible for observing any inhibition of the induction of genetic events, it would have inhibited all the end-points uniformly, but not just two out of three end-points studied. Fahim et al (2003) have reported the antitumour activities of IA and Dimethylsulphoxide against solid Ehrlich carcinoma growth in mice. They have reported an increase in the life span and tumour growth inhibition ratio to 145.7 and 63.8% by IA respectively.…”