Antitumor activity and antioxidant property of<i>Curcuma caesia</i>against Ehrlich’s ascites carcinoma bearing mice

Karmakar, Indrajit; Dolai, Narayan; Kumar, Rajesh; Kar, Biswakanth; Roy, Sudipendra Nath; Haldar, Pallab Kanti

doi:10.3109/13880209.2013.764538

Cited by 34 publications

(20 citation statements)

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“…20 On the basis of antioxidant activity in vitro cytotoxicity was checked for methanol extract of Zanthoxylum alatum (MEZA) by previously used trypan blue exclusion method. 6 The experiment was performed in triplicate. …”

Section: Determination Of In Vitro Cytotoxic Activitymentioning

confidence: 99%

“…Nitroblue tetrazolium (NBT), phenazine methosulphate (PMS), reduced nicotinamide adenine dinucleotide (NADH), sodium nitroprusside, napthyl ethylene diamine dihydrochloride, ascorbic acid, trichloroacetic acid (TCA), thiobarbituric acid (TBA), ethylene diamine tetra acetic acid (EDTA), sodium hydroxide (NaOH), hydrogen peroxide (H 2 O 2 ), deoxyribose, potassium ferricyanide [K 3 Fe(CN) 6 ], were purchased from Sisco Research Laboratories Pvt. Ltd., Mumbai, India.…”

Section: Chemicalsmentioning

confidence: 99%

“…5 Indeed, molecules derived from natural sources including plants, marine organisms and micro-organisms, have played, and continue to play, a dominant role in the discovery of leads for the development of conventional drugs for the treatment of most human diseases. 6 Zanthoxylum alatum Roxb. [Syn.…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant and Cytotoxic Activity of Different Extracts of Zanthoxylum Alatum

et al. 2015

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Objectives: The present study evaluates the antioxidant and cytotoxic activity of different extract (methanol, chloroform, petroleum ether) of Zanthoxylum alatum. Methodology: The antioxidant activities of all the three above mentioned extracts of Zanthoxylum alatum were measured by different in vitro standard methods like 1,1-diphenyl-2-picrylhydrazil radical (DPPH), superoxide anions, nitric oxide, hydroxyl radicals and reductive assay. On the basis of antioxidant properties total phenolic, total flavonoid, CCl 4 -induced in vivo antioxidant and in vitro cytotoxicity were measured for methanol extract of Zanthoxylum alatum (MEZA). Cytotoxicity was determined in Ehrlich Ascites Cancer (EAC) cells by trypan blue exclusion method. Results: The extracts exhibited antioxidant activities in a dose dependent manner. The IC 50 values of Methanol, Chloroform, Petroleum ether extracts and standard for DPPH are 3.63 ± 0.14 μg/mL, 9.33 ± 0.42 μg/mL, 7.70 ± 0.31 μg/mL and 7.87 ± 0.39 μg/mL; for Superoxide are 19.80 ± 0.96 μg/mL, 129.70 ± 2.57 μg/mL, 168.10 ± 2.26 μg/mL and 11.76 ± 0.72 μg/mL; for Nitric oxide are 105.0 ± 1.64 μg/mL, 157.60 ± 1.99 μg/mL, 185.30 ± 2.48 μg/mL and 28.48 ± 1.06 μg/mL; for Hydroxyl radical are 28.10 ± 0.75 μg/mL, 81.50 ± 1.88 μg/mL, 72.47 ± 1.76 μg/mL and 12.24 ± 0.82 μg/mL. Reductive ability of the extracts was also tested where dose dependent reducing capability was observed. In case of in vivo antioxidant lipid peroxidation, reduced glutathione, superoxide dismutase and catalase parameters significantly change towards the normal levels. MEZA showed satisfactory phenolic and flavonoid content which support the cytotoxicity with IC 50 value 102.30 ± 0.81 μg/mL. Conclusion: The study showed that among all the three extract MEZA has better dose dependant antioxidant and cytotoxic activity. Further research is going on to find out the active principle(s) of MEZA for its cytotoxic activity.

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Section: Determination Of In Vitro Cytotoxic Activitymentioning

confidence: 99%

Section: Chemicalsmentioning

confidence: 99%

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Antioxidant and Cytotoxic Activity of Different Extracts of Zanthoxylum Alatum

et al. 2015

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“…Percentage increase in life span (% ILS), tumor cell (viable/non-viable) count were calculated according to the standard methods (Karmakar et al, 2013).…”

Section: Tumor Related Parametersmentioning

confidence: 99%

“…Collected blood was used for the estimation of hemoglobin (Hb), red blood cell (RBC), and white blood cell (WBC) count by standard procedures (Karmakar et al, 2013).…”

Section: Hematological Parametersmentioning

confidence: 99%

Regulation of apoptosis through bcl-2/bax proteins expression and DNA damage byZanthoxylum alatum

Karmakar

Haldar

Chakraborty

et al. 2015

Pharmaceutical Biology

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Context: Many of the major chemotherapeutic agents are secondary metabolites found in nature. Zanthoxylum alatum Roxb. (Rutaceae) is traditionally used in the treatment of various diseases. Objective: The present study evaluates the apoptotic activity of methanol extract of Z. alatum (MEZA) on Ehrlich ascites tumor (EAT) in Swiss albino mice. Materials and methods: The presence of flavonoids in MEZA was standardized by HPLC. The in vitro cytotoxicity of MEZA was measured by the MTT assay. The in vivo antitumor activity of MEZA (100 and 200 mg/kg b.w., i.p. for 9 days) was also evaluated. On the 10th day, EAT tumor volume, cell viability, and hematological parameters were assayed. Apoptotic morphology was determined by acredine orange/ethedium bromide using fluorescence microscopy. Apoptosis percentage was measured by flow cytometric analysis using annexine-V-FITC. Also, DNA damage and bcl-2/bax were estimated by UV-method and western blot, respectively. Results and discussion: HPLC analysis revealed presence of three flavonoids, rutin, myricetin, and quercetin. MEZA showed satisfactory cytotoxicity in MTT assay (IC 50 ¼ 111.50 mg/ml). The extract significantly (p50.01) changed the tumor volume, viable, non-viable cell count, and hematological parameters towards the normal. Apoptotic activity of MEZA was confirmed by acridine orange/ethidium bromide staining, annexin-V-FITC staining, DNA fragmentation, and Bcl-2/Bax ratio. Conclusion: The study showed that MEZA has antitumor activity which may be due to the presence of flavonoids in the extract.

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Zingiberaceae Plants: A Cornucopia of Promising Chemotherapeuticals for Cancer Cure

Soumya,

Jayasree,

Manish Kumar

2023

Bioprospecting of Tropical Medicinal Plants

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Antitumor activity and antioxidant property ofCurcuma caesiaagainst Ehrlich’s ascites carcinoma bearing mice

Abstract: MECC possesses potent antitumor activity that may be due to its direct cytotoxic effect or antioxidant properties. Further research is in progress to find out the active principle(s) of MECC for its antitumor activity.

Cited by 34 publications

References 20 publications

Antioxidant and Cytotoxic Activity of Different Extracts of Zanthoxylum Alatum

Antioxidant and Cytotoxic Activity of Different Extracts of Zanthoxylum Alatum

Regulation of apoptosis through bcl-2/bax proteins expression and DNA damage byZanthoxylum alatum

Zingiberaceae Plants: A Cornucopia of Promising Chemotherapeuticals for Cancer Cure

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