Antitumor activity of hot-water extract from delipidated BCG.

et al. 1981

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A hot-water extract from delipidated Mycobacterium bovis strain BCG was fractionated by precipitation with ethanol. The chemical and biological properties of the subfractions thus obtained were investigated. A subfraction precipitated at 20% ethanol contained less neutral sugar and more ninhydrin-positive substance than other subfractions. Only the guinea pigs that were treated with 20% ethanol-precipitate showed no humoral antibody response to egg white albumin, despite positive skin reaction. The subfractions precipitated at low concentrations of ethanol exhibited a significant antitumor activity in ddY and C3H/He mice inoculated intraperitoneally with tumor cells. These results suggest that the hot-water extract from delipidated BCG consists of subfractions with different chemical and biological properties.immunotherapy; Mycobacterium bovis BCG; adjuvant activity A hot-water extract from delipidated BCG was shown to be adjuvantactive and to have antitumor activity (Sato et al. 1976). Several water-soluble, adjuvant-active substances isolated prior to this hot-water extract were of cell wall origin and the principal sugars in these adjuvants were arabinose and galactose. Chemical analyses of the above hot-water extract revealed that it included constituents from cytoplasm, since glucose and mannose were the principal neutral sugar components. However, small amounts of arabinose and trace of galactose have also been detected in the hot-water extract (Yokosawa 1978) and there remains a possibility that the adjuvant activity was due to the presence of cell wall fragments.Thus, the procedure of ethanol fractionation was applied in the present study in order to find out the principal structure responsible for adjuvant activity.The hot-water extract from BCG contained 50-60% of neutral sugar, 15-20% of ninhydrin-positive substance and about 10% of nucleic acid. Then the hot-water extract was fractionated into six subfractions by the method of fractional precipitation with ethanol which had been employed for the purification of polysaccharide of tubercle bacilli (Azuma et al. 1968).

Section: Antitumor Activitymentioning

confidence: 88%

Chemical and biological properties of subfractions of hot-water extract from delipidated BCG.

Motomiya

et al. 1981

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“…The hot water extract (HWE) from delipidated BCG has been found to be adjuvant-active and effective in the treatment of murine ascites tumors (Sato et al 1976(Sato et al , 1978(Sato et al , 1979. Also evidence for host-mediated antitumor activity of HWE has been presented (Sato et al 1982).…”

Section: Discussionmentioning

confidence: 99%

“…A hot water extract from delipidated BCG, which has been found to be derived from cytoplasm, is adjuvant-active and exhibits weak but significant antitumor activity (Sato et al 1976(Sato et al , 1978(Sato et al , 1979.…”

mentioning

confidence: 99%

Chemical analyses and antitumor activity of hydrosoluble substances from Mycobacterium bovis, strain BCG.

Nagai

et al. 1983

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--Hydrosoluble substances from BCG were prepared by cold water extraction and by hot phenol-water extraction. Chemical analyses revealed that both of them were derived from cytoplasm. The cold water extract (CWE) was effective in the treatment of C3H/He mice which had received an intraperitoneal inoculation of a syngeneic ascites hepatoma, MH134. The growth of a graft in footpad of mastocytoma P815 in CDF1 mice was retarded by intraperitoneal injections of CWE. A peptidoglycan from cell wall prepared by digestion with lysozyme exerted no antitumor activity in the same experimental condition as for the evaluation of antitumor effect of CWE. These results indicate that the antitumor activity of CWE was not due to the presence of a cell wall component, ----hydrosoluble substance; BCG; antitumor activity; mastocytoma P815There are a number of publications dealing with the effects of mycobacteria on immune response and on antitumor activity. Several fractions of mycobacteria have been investigated in order to identify the chemical constituents which are responsible for these activities and as a result cell wall has been found to have these biological activities.A hot water extract from delipidated BCG, which has been found to be derived from cytoplasm, is adjuvant-active and exhibits weak but significant antitumor activity (Sato et al. 1976(Sato et al. , 1978(Sato et al. , 1979.In this study, a cold water extract from delipidated BCG and a phenolwater extract were prepared to compare their antitumor effects with those of a water soluble substance from cell wall isolated by an enzymatic treatment of BCG.This report deals with the chemical analyses and antitumor activity of the above hydrosoluble substances.

“…These results indicate the participation of hostmediated antitumor activity when neoplasms were treated with HWE. -aqueous extract from BCG; antitumor activity; silica; carragheenan; hydrocortisone acetate A hot water extract (HWE) from delipidated Mycobacterium bovis, strain BOG, was found to be adjuvant-active and effective in the treatment of ddY mice inoculated intraperitoneally with Sarcoma 180 or Ehrlich carcinoma (Sato et al 1976(Sato et al , 1978(Sato et al , 1979Yokosawa 1978). A slight prolongation of survival time (statistically significant) was observed when syngeneic, ascites hepatoma-bearing 0311/He mice were treated with intraperitoneal injections of HWE (Motomiya et al 1981).…”

mentioning

confidence: 99%

Evidence for host-mediated antitumor activity in the treatment of experimental tumor with a hot water extract from BCG.

Nagai

et al. 1982

Self Cite

M. and K0NN0, K. Evidence for Host-Mediated Antitumor Activity in the Treatment o f Experimental Tumor with a Hot Water Extract from BCG. Tohoku J. exp. Med., 1982, 136 (1), 67-74 Studies were undertaken for the evaluation of the mechanism responsible for antitumor effects of hot water extract (HWE) of BCG on murine ascites tumor cells, a) The viability of tumor cells has remained unchanged after incubation of tumor cells in the medium containing HWE. b) There was no difference in survival time between mice which had received tumor cells treated as in (a) and the control mice. c) Silica, carragheenan and cortisone acetate reduced the antitumor effect of HWE in ddY mice and C3H/He mice which had been inoculated with Sarcoma 180 cells or MH134 cells, d) The growth of tumor cells was retarded when they were inoculated subcutaneously together with peritoneal cells from HWE-injected mice. These results indicate the participation of hostmediated antitumor activity when neoplasms were treated with HWE. -aqueous extract from BCG; antitumor activity; silica; carragheenan; hydrocortisone acetate A hot water extract (HWE) from delipidated Mycobacterium bovis, strain BOG, was found to be adjuvant-active and effective in the treatment of ddY mice inoculated intraperitoneally with Sarcoma 180 or Ehrlich carcinoma (Sato et al. 1976(Sato et al. , 1978(Sato et al. , 1979Yokosawa 1978). A slight prolongation of survival time (statistically significant) was observed when syngeneic, ascites hepatoma-bearing 0311/He mice were treated with intraperitoneal injections of HWE (Motomiya et al. 1981).In this study, experiments were undertaken to elucidate the mechanism responsible for the activity of HWE from BOG against tumor cells. The effect of silica, carragheenan and hydrocortisone acetate on antitumor activity of HWE was investigated in mice which had been inoculated intraperitoneally with allogeneic or syngeneic tumor cells.