Antitumor activity of intralesionally administered Nocardia opaca preparations in rat and mouse tumors: A comparison with BCG and corynebacterium parvum

Barot‐Ciorbaru, R.; Petit, Jean‐François; Chassoux, D.; Salomon, Jean-Claude

doi:10.1016/0192-0561(81)90001-1

Cited by 10 publications

(4 citation statements)

References 18 publications

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“…We have previously demonstrated that the immunomodulatory fractions of N. opaca, NDCM, NWSM, and Nocardia lysozyme digest had potent antitumor activities against rat and mouse fibrosarcomas [3]. In the present study, (1) we extended these investigations to tumors which give rise to lung metastases; (2) we examined the synergy between monokines and Nocardia fractions; and (3) we studied the correlation between antimetastatic activity in vivo and macrophage activation.…”

Section: Discussionmentioning

confidence: 95%

“…In particular, under carefully defined conditions they have been able to induce the production of interferon [4], activate natural killer lymphocytes (NK) [7] and macrophages [5,25], induce antitumoral activity [3], and allow the detection of macrophages present in the vicinity of human tumors and metastases [25]. Treatment of resident peritoneal macrophages from mice or rats with these fractions stimulated secretion of interleukin 1 and other monokines [5].…”

Section: Introductionmentioning

confidence: 99%

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Antimetastatic effect of immunomodulators from Nocardia opaca in mice and rats activation of peritoneal macrophages by these fractions

Barot‐Ciorbaru

Cornil²,

Grand-Perret

et al. 1987

Cancer Immunol Immunother

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Nocardia delipidated cell mitogen (NDCM), a particulate fraction prepared from Nocardia opaca, injected i.p. in an oil/water emulsion to F6 rhabdomyosarcoma-bearing rats, inhibited the development of pulmonary metastases; 6 out of 10 rats were protected. Repeated i.p. administration of emulsified NDCM and of two other compounds, a Nocardia water soluble mitogen (NWSM a hydrosoluble fraction) and purified cell walls (CW, an insoluble macromolecular fraction) in Lewis lung carcinoma (LLC)-bearing mice resulted in a significant reduction of lung metastases. The efficiency of these fractions was enhanced by association with monokines. A combination regimen of NDCM, NWSM, and CW (100 micrograms/0.1 ml) and monokines (0.1 ml), injected i.p. in LLC-bearing mice, yielded a greater antimetastatic effect than either therapy alone. Peritoneal macrophages from mice which had been injected i.p. with NWSM or CW, when triggered either by TPA (tetradecanoyl phorbol acetate) or by zymosan, released large quantities of hydrogen peroxide and had a high rate of glucose consumption. These macrophages were activated as judged by their cytostatic activity against syngeneic P815 mastocytoma growth; they expressed biochemical markers which have been reported to characterize the activated state. Incubation of thioglycollate-elicited peritoneal macrophages with NWSM, and monokines for 72 h resulted in a cytotoxic activity against labeled LLC cells; addition of macrophage activating factor significantly increased the cytotoxic capacity of these macrophages. In view of this we postulate that the antimetastatic effect of soluble and insoluble N. opaca fractions and monokines might be mediated by activated peritoneal macrophages.

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Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Antimetastatic effect of immunomodulators from Nocardia opaca in mice and rats activation of peritoneal macrophages by these fractions

Barot‐Ciorbaru

Cornil²,

Grand-Perret

et al. 1987

Cancer Immunol Immunother

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“…Immunostimulants exhibiting such effects include various synthetic products, i.e., levamisole [8,20,21], poly I : C [21], and bacteria and bacterial products, e.g., Bacillus Calmette-Gu&in (BCG) [3,13,14,17], Corynebacterium parvum [6,15], Salmonella typhimurium [11,12], genus Nocardia [1,2,4]. The antitumor activities of some of these agents have been far from satisfactory, and the side-effects of some of the agents are severe [22].…”

Section: Introductionmentioning

confidence: 99%

Augmentation of antitumor resistance by a strain of unicellular green algae, Chlorella vulgaris

Tanaka

Konishi

Himeno

et al. 1984

Cancer Immunol Immunother

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Growth of Meth-A tumor in CDF1 mice was inhibited significantly by injection of a hot water extract of a strain of Chlorella vulgaris (CE) into the tumor or into the subcutaneous tissue near the tumor. The augmentation of resistance by CE may require the participation of T cells and macrophages, since it was abolished or reduced in athymic nude mice or mice treated with carrageenan, a macrophage blocker. Mice treated with CE exhibited antigen-specific augmented resistance against rechallenge with tumor. Moreover, the antitumor effect of CE was comparable with that of Corynebacterium parvum, but its mechanism of effect might be different.

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“…Isolated fractions of Nocardia were shown to exert adjuvant effect [2], to induce regression of experimental tumours [3][4][5][6][7], to enhance protection against irradiation [8,9], and to promote resistance to infections [10]. These activities include activation of natural killer (NK) cells and macrophages, followed by enhanced cytotoxicity of these cells and production of tumour necrosis factor-alpha (TNF-), IL-1 and other cytokines [3,[11][12][13][14][15][16][17] and stimulation of B cells to proliferation and differentiation into immunoglobulin-secreting cells [1,2,10,18,19].…”

Section: Introductionmentioning

confidence: 99%

Macrophage nitric oxide synthase (NOS) activation by Nocardia opaca fractions and 15- and 56-kD isolated antigens

Tučková

Zı́dek

Hanikýřová

et al. 1996

Clinical and Experimental Immunology

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SUMMARYThe Gram-positive bacterium, Nocardia opaca, is a source of substances with adjuvant effect, ability to stimulate macrophages and natural killer cells for enhanced cytotoxity and cytokine production and B lymphocytes for polyclonal immunoglobulin secretion. We determined the immunogenicity of isolated N. opaca fractions and prepared MoAbs against immunogenic water-soluble mitogen (NWSM). Two main proteins of molecular mass 15 and 56 kD were detected in Western blot analysis and isolated by affinity chromatography using anti-NWSM MoAb B7/7. Both these isolated nocardial antigens were found to stimulate mouse peritoneal macrophage NOS. The effect of 5 ¹ g NWSM was comparable to that of 5 ¹ g lipopolysaccharide (LPS) or 20 U of interferon-gamma (IFN-°) added to cell cultures. The MoAb B7/7 decreased NO 2 ÿ production induced by NWSM or by isolated nocardial antigens, but did not significantly influence the production elicited by LPS or IFN-°. On the other hand, NOS activation by NWSM was not affected by anti-IFN-°MoAb. The possible independent pathway for IFN-°and NWSM macrophage activation is discussed.

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Antitumor activity of intralesionally administered Nocardia opaca preparations in rat and mouse tumors: A comparison with BCG and corynebacterium parvum

Cited by 10 publications

References 18 publications

Antimetastatic effect of immunomodulators from Nocardia opaca in mice and rats activation of peritoneal macrophages by these fractions

Antimetastatic effect of immunomodulators from Nocardia opaca in mice and rats activation of peritoneal macrophages by these fractions

Augmentation of antitumor resistance by a strain of unicellular green algae, Chlorella vulgaris

Macrophage nitric oxide synthase (NOS) activation by Nocardia opaca fractions and 15- and 56-kD isolated antigens

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