Antitumor‑ and apoptosis‑inducing effects of pomolic acid against SK‑MEL‑2 human malignant melanoma cells are mediated via inhibition of cell migration and sub‑G1 cell cycle arrest

Li, Tian‐Hang; Yan, Hongxia

doi:10.3892/mmr.2017.7977

Cited by 3 publications

(3 citation statements)

References 20 publications

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“…The increased levels of these factors in pomolic acid-treated A549 cells indicated onset of apoptosis. The results of the present study are similar to those reported by Li & Yan [16].…”

Section: Discussionsupporting

confidence: 93%

Pomolic acid inhibits proliferation of human lung carcinoma cells via induction of apoptosis and suppression of cell migration and invasion

Yang¹,

Guo²,

Hao³

et al. 2022

Trop. J. Pharm Res

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Purpose: To investigate the anti-proliferative effect of pomolic acid on lung cancer cells (A549), and theunderlying mechanism.Methods: The viability of pomolic acid-treated A549 cells was determined by MTT and colony formation assays. Cell colony formation was monitored with acridine orange/ethidium bromide (AO/EB) staining. Protein expressions of Bax and Bcl-2 were assayed by western blotting.Results: Pomolic acid suppressed the growth of A549 cells, with an half-maximal inhibitory concentration of (IC50) of 10 μM (p < 0.05). However, the IC50 of pomolic acid for normal BEAS-2B cells was 80 μM. Pomolic acid also decreased colony formation of A549 cells. At 20 μM, the percentage of A549 colonies decreased to 14 % of control. The dose-dependent cytotoxicity of pomolic acid against A549 cells was mediated via induction of apoptosis and oxidative stress. Pomolic acid treatment enhanced the expression of Bax and decreased the expression of Bcl-2 in A549 cells. Moreover, pomolic acid inhibited the migration and invasion in A549 cells in a dose-dependent manner (p < 0.05).Conclusion: These results indicate the potent anticancer effect of pomolic acid against human lung cancer cells. Thus, pomolic acid has promising potential as a lead molecule for the development of chemotherapy.

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“…The increased levels of these factors in pomolic acid-treated A549 cells indicated onset of apoptosis. The results of the present study are similar to those reported by Li & Yan [16].…”

Section: Discussionsupporting

confidence: 93%

Pomolic acid inhibits proliferation of human lung carcinoma cells via induction of apoptosis and suppression of cell migration and invasion

Yang¹,

Guo²,

Hao³

et al. 2022

Trop. J. Pharm Res

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“…It was further confirmed that stigmasterol also suppressed neoplasm cell migration and facilitated G2/M cell cycle stagnation. In addition, stigmasterol can regulate the JAK/STAT channel [63] to exert anticancer activity. Research has shown that stigmasterol inhibits lung neoplasm cell reproduction and promotes apoptosis.…”

Section: Stigmasterolmentioning

confidence: 99%

Anti-Tumor Effects and Toxicity Reduction Mechanisms of Prunella vulgaris: A Comprehensive Review

Ning,

Nan,

Chen

et al. 2024

Molecules

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Purpose: To investigate and systematically describe the mechanism of action of Prunella vulgaris (P. vulgaris) against digestive system tumors and related toxicity reduction. Methods: This study briefly describes the history of medicinal food and the pharmacological effects of P. vulgaris, focusing on the review of the anti-digestive tumor effects of the active ingredients of P. vulgaris and the mechanism of its toxicity reduction. Results: The active ingredients of P. vulgaris may exert anti-tumor effects by inducing the apoptosis of cancer cells, inhibiting angiogenesis, inhibiting the migration and invasion of tumor cells, and inhibiting autophagy. In addition, P. vulgaris active ingredients inhibit the release of inflammatory factors and macrophages and increase the level of indicators of oxidative stress through the modulation of target genes in the pathway to achieve the effect of toxicity reduction. Conclusion: The active ingredients in the medicine food homology plant P. vulgaris not only treat digestive system tumors through different mechanisms but also reduce the toxic effects. P. vulgaris is worthy of being explored more deeply.

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“…To get a better insight into the cells' death modalities, we have applied AO/PI cell staining method. First, HeLa cells were stained with acridine orange (AO) and propidium iodide (PI) according to standard procedures [27,28]. Next, stained HeLa cells were analyzed under an epifluorescent microscope Axioimager A1 (Carl Zeiss, Jena, Germany) equipped with the ISIS imaging software package (Meta-Systems, Altlussheim, Germany).…”

Section: Cells' Death Modalities Induced By C-tio 2 And/ or Blue Lightmentioning

confidence: 99%

Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles

Matijević

Žakula

Korićanac

et al. 2021

Photochem Photobiol Sci

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In this study, C-doped TiO 2 nanoparticles (C-TiO 2 ) were prepared and tested as a photosensitizer for visible-light-driven photodynamic therapy against cervical cancer cells (HeLa). X-ray diffraction and Transmission Electron Microscopy confirmed the anatase form of nanoparticles, spherical shape, and size distribution from 5 to 15 nm. Ultraviolet-visible light spectroscopy showed that C doping of TiO 2 enhances the optical absorption in the visible light range caused by a bandgap narrowing. The photo-cytotoxic activity of C-TiO 2 was investigated in vitro against HeLa cells. The lack of dark cytotoxicity indicates good biocompatibility of C-TiO 2 . In contrast, a combination with blue light significantly reduced the survival of HeLa cells: illumination only decreased cell viability by 30% (15 min of illumination, 120 µW power), and 60% when HeLa cells were preincubated with C-TiO 2 . We have also confirmed blue light-induced C-TiO 2 -catalyzed generation of reactive oxygen species in vitro and intracellularly. Oxidative stress triggered by C-TiO 2 /blue light was the leading cause of HeLa cell death. Fluorescent labeling of treated HeLa cells showed distinct morphological changes after the C-TiO 2 /blue light treatment. Unlike blue light illumination, which caused the appearance of large necrotic cells with deformed nuclei, cytoplasm swelling, and membrane blebbing, a combination of C-TiO 2 /blue light leads to controlled cell death, thus providing a better outcome of local anticancer therapy.

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Antitumor‑ and apoptosis‑inducing effects of pomolic acid against SK‑MEL‑2 human malignant melanoma cells are mediated via inhibition of cell migration and sub‑G1 cell cycle arrest

Cited by 3 publications

References 20 publications

Pomolic acid inhibits proliferation of human lung carcinoma cells via induction of apoptosis and suppression of cell migration and invasion

Pomolic acid inhibits proliferation of human lung carcinoma cells via induction of apoptosis and suppression of cell migration and invasion

Anti-Tumor Effects and Toxicity Reduction Mechanisms of Prunella vulgaris: A Comprehensive Review

Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles

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