Antitumor and immunomodulatory activity of genkwanin on colorectal cancer in the APC Min/+ mice

Wang, Xue; Song, Zijing; He, Xin; Zhang, Runqi; Zhang, Chunfeng; Li, Fēi; Wang, Chong‐Zhi; Yuan, Chun‐Su

doi:10.1016/j.intimp.2015.09.006

Cited by 48 publications

(28 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Colorectal cancer (CRC) is the third most commonly occurring malignancy around the world with significant morbidity and mortality rates. Every year, 1.2 million people are diagnosed with CRC [1][2][3][4]. Evasion of apoptosis is one of the hallmarks of cancer, in general, and correlated to drug resistance and metastatic spread, which urges the development of new drugs [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Effect of Oxaliplatin-Loaded Poly (d,l-Lactide-co-Glycolic Acid) (PLGA) Nanoparticles Combined with Retinoic Acid and Cholesterol on Apoptosis, Drug Resistance, and Metastasis Factors of Colorectal Cancer

et al. 2020

View full text Add to dashboard Cite

Apoptosis signaling pathways, drug resistance, and metastasis are important targets to develop new cancer treatments. We developed cholesterol-coated Poly(d,l-Lactide-co-Glycolic Acid) (PLGA) nanoparticles for effective encapsulation and delivery of retinoic acid and oxaliplatin to analyze their antitumor activity in colorectal cancer. The cell viability and proliferation of tumoral cells lines (CT-26 and SW-480) decreased when compared to control in vitro after treatment with the nanoparticles. In addition, apoptosis of CT-26 cells increased. Importantly, cytoprotection of nontumor cells was detected. Expression of pro-apoptotic proteins was upregulated, while anti-apoptotic proteins were downregulated either in vitro or in vivo. In addition, drug resistance and metastasis factors were downregulated in vivo. Human colorectal tumors that highly expressed BCL-2 and Ki-67 had a greater tendency towards death within 60 months. Our results show that loading oxaliplatin combined with retinoic acid and cholesterol in a nanoparticle formulation enables determination of optimal antitumor activity and subsequent treatment efficacy.

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of Oxaliplatin-Loaded Poly (d,l-Lactide-co-Glycolic Acid) (PLGA) Nanoparticles Combined with Retinoic Acid and Cholesterol on Apoptosis, Drug Resistance, and Metastasis Factors of Colorectal Cancer

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Apigenin possesses anti‐inflammatory, anti‐oxidant and anticancer activities (Gaballah, Gaber, & Mohamed, ; Patil et al, ; Xu et al, ). Genkwanin shows significant anti‐tumor activity by enhancing host immunity and decreasing inflammatory cytokine levels (Wang et al, ); this compound also exhibits antioxidant properties and immunomodulatory effects on splenocytes, NK and CTL cells (Nasr‐Bouzaiene et al, ). Hydroxygenkwanin possesses anti‐glioma and anti‐rheumatoid arthritic activities (Jiang et al, ; Wang et al, ).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of three flavonoids and one coumarin by LC–MS/MS: Application to a comparative pharmacokinetic study in normal and arthritic rats after oral administration of Daphne genkwa extract

Zhou

Yan

2018

Biomedical Chromatography

View full text Add to dashboard Cite

A selective and sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for investigating the pharmacokinetics of umbelliferone, apigenin, genkwanin and hydroxygenkwanin after oral administration of Daphne genkwa extract. Plasma samples were treated by protein precipitation with acetonitrile. Analytes were detected by triple-quadrupole MS/MS with an ESI source in negative selection reaction monitoring mode. The transitions of m/z 161 → 133 for umbelliferone, m/z 269 → 117 for apigenin, m/z 283 → 268 for genkwanin and m/z 299 → 284 for hydroxygenkwanin were confirmed for quantification. Chromatographic separation was conducted using an Eclipse XDB-C column, and the applied isocratic elution program allowed for simultaneous determination of the four analytes for a total run time of 2.5 min. The linearity was validated over the plasma concentration ranges of 1.421-1421 ng/mL for umbelliferone, 0.845-845 ng/mL for apigenin, 1.025-1025 ng/mL for genkwanin and 0.845-845 ng/mL for hydroxygenkwanin. The extraction recovery rate was >82.7% for each analyte. No apparent matrix effect was observed during the bioanalysis. After full validation, the proposed method was successfully applied to compare the pharmacokinetics of these analytes between normal and arthritic rats.

show abstract

“…Some of the compounds have been shown to possess pharmacologic activities for the treatment of various diseases. These include the anti-lung-cancer activity of isorhamnetin ( 25 ), as well as beta-sitosterol (analgesic) ( 26 ), genkwanin (anti-colorectal cancer) ( 27 ), eupalitin (anti-prostate carcinoma) ( 28 ), capillarisin (anti-hyperalgesic and anti-allodynic) ( 29 ), quercetin (anticancer) ( 30 ), rhein (anticancer) ( 31 ), aloe-emodin (anti-growth disorders) ( 32 ), crocetin (anticancer) ( 33 ), Sudan III (anti-persistent chylous ascites) ( 34 ), kaempferol (anti-pancreatic cancer) ( 35 ), stigmasterol (anticancer) ( 36 ). In our study, based on the potential targets that 34 compounds act upon, 31 significant pathways and 16 classes of diseases that were associated with the targets were obtained.…”

Section: Discussionmentioning

confidence: 99%

Identification of the active compounds and significant pathways of yinchenhao decoction based on network pharmacology

Huang

Cheung

Tan

et al. 2017

Molecular Medicine Reports

117

101

View full text Add to dashboard Cite

Yinchenhao decoction (YCHD) is a traditional Chinese medicine formulation, which has been widely used for the treatment of jaundice for 2,000 years. Currently, YCHD is used to treat various liver disorders and metabolic diseases, however its chemical/pharmacologic profiles remain to be elucidated. The present study identified the active compounds and significant pathways of YCHD based on network pharmacology. All of the chemical ingredients of YCHD were retrieved from the Traditional Chinese Medicine Systems Pharmacology database. Absorption, distribution, metabolism and excretion screening with oral bioavailability (OB) screening, drug-likeness (DL) and intestinal epithelial permeability (Caco-2) evaluation were applied to discover the bioactive compounds in YCHD. Following this, target prediction, pathway identification and network construction were employed to clarify the mechanism of action of YCHD. Following OB screening, and evaluation of DL and Caco-2, 34 compounds in YCHD were identified as potential active ingredients, of which 30 compounds were associated with 217 protein targets. A total of 31 significant pathways were obtained by performing enrichment analyses of 217 proteins using the JEPETTO 3.x plugin, and 16 classes of gene-associated diseases were revealed by performing enrichment analyses using Database for Annotation, Visualization and Integrated Discovery v6.7. The present study identified potential active compounds and significant pathways in YCHD. In addition, the mechanism of action of YCHD in the treatment of various diseases through multiple pathways was clarified.

show abstract

Antitumor and immunomodulatory activity of genkwanin on colorectal cancer in the APC Min/+ mice

Cited by 48 publications

References 27 publications

Effect of Oxaliplatin-Loaded Poly (d,l-Lactide-co-Glycolic Acid) (PLGA) Nanoparticles Combined with Retinoic Acid and Cholesterol on Apoptosis, Drug Resistance, and Metastasis Factors of Colorectal Cancer

Effect of Oxaliplatin-Loaded Poly (d,l-Lactide-co-Glycolic Acid) (PLGA) Nanoparticles Combined with Retinoic Acid and Cholesterol on Apoptosis, Drug Resistance, and Metastasis Factors of Colorectal Cancer

Simultaneous determination of three flavonoids and one coumarin by LC–MS/MS: Application to a comparative pharmacokinetic study in normal and arthritic rats after oral administration of Daphne genkwa extract

Identification of the active compounds and significant pathways of yinchenhao decoction based on network pharmacology

Contact Info

Product

Resources

About