Antitumor effect of 5-fluorouracil is enhanced by rosemary extract in both drug sensitive and resistant colon cancer cells

González-Vallinas, Margarita; Molina, Susana; Vicente, G.; Cueva, Ana de la; Vargas, Teodoro; Santoyo, Susana; García-Risco, Mónica Rodríguez; Fornari, Tiziana; Reglero, Guillermo; Molina, Ana Ramı́rez de

doi:10.1016/j.phrs.2013.03.010

Cited by 88 publications

(72 citation statements)

References 20 publications

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“…Cell viability was determined by the 3-(4,5-dimethyl-thyazol-2-yl)-2,5-diphenyl-tetrazolium (MTT) assay as previously described [16]. Briefly, 20 000–30 000 cells were seeded in 24-well plates, attached overnight, and treated with increasing concentrations of RE's.…”

Section: Methodsmentioning

confidence: 99%

“…In order to properly apply rosemary as a nutritional supplement for cancer therapy, additional information regarding the most effective composition, its antitumor effect in vivo and its main molecular mediators is still needed. In this sense, we have previously reported the synergistic effect of the combination of supercritical fluid rosemary extract and 5-fluorouracil, the most commonly used drug in colon cancer therapy, through the modulation of TK1 and TYMS, which are enzymes related to the mechanism of action of this drug [16].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, they exert antioxidant activity both in vitro [5], [6] and in vivo [7], thus inhibiting genotoxicity, which is a significant contributory cause of cancer, and protecting from carcinogens or toxic agents. They also were reported to display antiproliferative activity in vitro against breast [8]–[10], leukemia [8], [9], [11], hepatoma [9], [11]–[13], colon [11], [14]–[16], lung [9], prostate [9], ovarian [13], [17], and urinary bladder [10] cancer cells. However, the effect of rosemary on pancreatic carcinoma cells has not been reported to date.…”

Section: Introductionmentioning

confidence: 99%

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Expression of MicroRNA-15b and the Glycosyltransferase GCNT3 Correlates with Antitumor Efficacy of Rosemary Diterpenes in Colon and Pancreatic Cancer

et al. 2014

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Colorectal and pancreatic cancers remain important contributors to cancer mortality burden and, therefore, new therapeutic approaches are urgently needed. Rosemary (Rosmarinus officinalis L.) extracts and its components have been reported as natural potent antiproliferative agents against cancer cells. However, to potentially apply rosemary as a complementary approach for cancer therapy, additional information regarding the most effective composition, its antitumor effect in vivo and its main molecular mediators is still needed. In this work, five carnosic acid-rich supercritical rosemary extracts with different chemical compositions have been assayed for their antitumor activity both in vivo (in nude mice) and in vitro against colon and pancreatic cancer cells. We found that the antitumor effect of carnosic acid together with carnosol was higher than the sum of their effects separately, which supports the use of the rosemary extract as a whole. In addition, gene and microRNA expression analyses have been performed to ascertain its antitumor mechanism, revealing that up-regulation of the metabolic-related gene GCNT3 and down-regulation of its potential epigenetic modulator miR-15b correlate with the antitumor effect of rosemary. Moreover, plasmatic miR-15b down-regulation was detected after in vivo treatment with rosemary. Our results support the use of carnosic acid-rich rosemary extract as a complementary approach in colon and pancreatic cancer and indicate that GCNT3 expression may be involved in its antitumor mechanism and that miR-15b might be used as a non-invasive biomarker to monitor rosemary anticancer effect.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Expression of MicroRNA-15b and the Glycosyltransferase GCNT3 Correlates with Antitumor Efficacy of Rosemary Diterpenes in Colon and Pancreatic Cancer

et al. 2014

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“…Quercetin increased the bioavailability of pioglitazone in rats by inhibiting CYP3A (116). Rosemary extract enhanced antitumor effect of 5-fluorouracil by downregulation of thymidylate synthetase enzyme and TK1 genes, which are essential for the synthesis of dTMP related to 5-fluorouracil resistance (117). Agosterol A, which is polyhydroxylated sterol acetate isolated from marine sponge, showed strong activity by completely reversing the resistance against vincristine, colchicine, doxorubicin, and etoposide in KB-C2 and KB-CV60 cells.…”

Section: Kim Et Al Reported That Resveratrol Enhanced Doxorubicinindmentioning

confidence: 99%

Natural Product Modulators to Overcome Multidrug Resistance In Cancer

Çört

Özben

2015

Nutrition and Cancer

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Multidrug resistance (MDR) is a condition that makes cells simultaneously unresponsive to different drugs, unrelated to their chemical structure and mechanism of action. MDR caused by the presence and overexpression of ABC transporters makes obstacles in cancer treatment and lower the effectiveness of chemotherapy. Natural products are investigated by many researchers as MDR modulators for their low toxicity and potent, selective behavior. When coadministered, MDR modulators compete with cytotoxic agents for binding to the active site of the membrane transporters and reduce drug efflux. Natural product-based drugs are important in struggling against drug resistance during cancer therapy. This review is focused on the potential mechanisms against drug resistance, the development of inhibitors for ABC drug transporters, natural product modulators, and nanoparticle drug delivery.

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“…It possesses needle-like leaves which contain a powerful fragrance 2 . It is used as a food flavoring agent, and well known medicinally for its powerful antitumor, antibacterial, antifungal and antioxidant activity [3][4][5][6][7] .Rosemary essential oil composition and the ratio between different oil constituents plays a role in its bioactivity 8 . Rosemary oil composition depends on various environmental and genetic factors, as well as on postharvest processing factors 9 .…”

Section: Rosemary (Rosmarinus Officinalis L) Is Amentioning

confidence: 99%

Supercritical Fluid Extraction as a Technique to Obtain Essential Oil from Rosmarinus officinalis L.

Khalili¹,

Mazloomifar²,

Larijani³

et al. 2017

Orient. J. Chem

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The aim of this study was the comparison of essential oil of one herb from lamiaceae family namely Rosemary, via supercritical fluid extraction (SFE) and hydrodistillation (HD). The essential oils were analyzed by gas chromatography-quadrupole mass spectrometry (GC-MS). The compounds identified in higher relative abundance were,α-pinene (HD: 30.42%, SFE: 0.47%), 1,8-cineole (HD: 15.46%, SFE: 25.05%),Verbenone (HD: 13.09%, SFE: 5.17%), Camphor (HD: 8.97%, SFE:15.69%), Borneol (HD: 7.16%, SFE: 9.15%) and β∋-Caryophyllene (SFE: 6.85%).

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Antitumor effect of 5-fluorouracil is enhanced by rosemary extract in both drug sensitive and resistant colon cancer cells

Cited by 88 publications

References 20 publications

Expression of MicroRNA-15b and the Glycosyltransferase GCNT3 Correlates with Antitumor Efficacy of Rosemary Diterpenes in Colon and Pancreatic Cancer

Expression of MicroRNA-15b and the Glycosyltransferase GCNT3 Correlates with Antitumor Efficacy of Rosemary Diterpenes in Colon and Pancreatic Cancer

Natural Product Modulators to Overcome Multidrug Resistance In Cancer

Supercritical Fluid Extraction as a Technique to Obtain Essential Oil from Rosmarinus officinalis L.

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