Antitumor Effect of All-Trans Retinoic Acid-Encapsulated Nanoparticles of Methoxy Poly(Ethylene Glycol)-Conjugated Chitosan Against CT-26 Colon Carcinoma In Vitro

The synthesized 2-(2-fluorophenyl)-6,7-methylenedioxyquinolin-4-one (CHM-1) promoted G2/M arrest through inhibition of CDK1 and induced apoptosis through the mitochondrial-dependent pathway in CT-26 murine colorectal adenocarcinoma cells

“…Cells were cultured in RPMI-1640 medium (GIBCO, Grand Island, NY, USA) supplemented with 10% heat-inactivated fetal calf serum (Hyclone, Logan, UT, USA) at 37°C in a humidified 5% CO 2 and 95% air at 1 atm incubator. Full grown monolayer cultures were trypsinized (0.25% trypsin-EDTA), harvested and passaged several times for expansion [13].…”

Nanochitosan: Commemorating the Metamorphosis of an ExoSkeletal Waste to a Versatile Nutraceutical

“…The release of 5-aminosalicylic acid from nanochitosan based on ion-exchange mechanism is reported [156]. S-DNA chitosan nanoparticles were more stable in the upper regions of the small intestine [157] and have been reported for targeting drugs to colon tumor [158] and have been also reported for endoscopic detection of colorectal cancer [159] and apoptosis initiation by transretinoic acid bearing methoxy poly(ethylene glycol)-grafted nanochitosan [160]. Moreover, hyaluronic acid coupled CSNPs were 60-fold more effective on HT-29 cells [161].…”

Nanoparticles Based on Modified Polysaccharides

“…Therefore, poly (ethylene glycol) is widely used as a pharmacological polymer with high hydrophilicity, biocompatibility and biodegradability. In recent years, derivative poly (ethylene glycol)-g-derivative chitosan to obtain nanoparticles has been studied by many researchers (Ouchi et al, 1998;Jung et al, 2006) (Park et al, 2008) (Yang et al, 2008b) (Opanasopit et al, 2007). The grafted poly (ethylene glycol) methyl ether onto N-Phthaloyl chitosan chains, aggregated to obtain sphere-like nanoparticles (an et al, 2004).…”