Antitumor effect of human TRAIL on adenoid cystic carcinoma using magnetic nanoparticle–mediated gene expression

Miao, Leiying; Zhang, Kai; Qiao, Chen; Jin, Xuesong; Zheng, Changyu; Yang, Bai; Sun, Haiming

doi:10.1016/j.nano.2012.04.006

Cited by 25 publications

(22 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…48 In another study, they also demonstrated the antitumor effect of magnetofection with plasmid DNA encoding tumor necrosis factor-related apoptosis-inducing ligand in adenoid cystic carcinoma in mice. 49 In our previous study, the magnetofection of TS/A tumors in mice with therapeutic plasmid DNA encoding IL-12 resulted also in a significant antitumor effect, which was comparable to gene electrotransfer. 11 …”

Section: Discussionmentioning

confidence: 87%

“…Only three other research groups had used magnetofection for the treatment of tumors in vivo, and demonstrated its efficiency, safety and potential use for the delivery of therapeutic genes. 11,46,47,48,49 However, only in two studies was antitumor effect of magnetofection with therapeutic genes demonstrated. In the first study magnetofection was investigated in combination with RNAi, similar to our study.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Mcam Silencing With RNA Interference Using Magnetofection has Antitumor Effect in Murine Melanoma

Prosen

Markelc

Dolinšek

et al. 2014

Molecular Therapy - Nucleic Acids

View full text Add to dashboard Cite

The melanoma cell adhesion molecule (MCAM) is involved in melanoma development and its progression, including invasiveness, metastatic potential and angiogenesis. Therefore, MCAM represents a potential target for gene therapy of melanoma, whose expression could be hindered with posttranscriptional specific gene silencing with RNA interference technology. In this study, we constructed a plasmid DNA encoding short hairpin RNA against MCAM (pMCAM) to explore the antitumor and antiangiogenic effects. The experiments were performed in vitro on murine melanoma and endothelial cells, as well as in vivo on melanoma tumors in mice. The antiproliferative, antimigratory, antiangiogenic and antitumor effects were examined after gene therapy with pMCAM. Gene delivery was performed by magnetofection, and its efficacy compared to gene electrotransfer. Gene therapy with pMCAM has proved to be an effective approach in reducing the proliferation and migration of melanoma cells, as well as having antiangiogenic effect in endothelial cells and antitumor effect on melanoma tumors. Magnetofection as a developing nonviral gene delivery system was effective in the transfection of melanoma cells and tumors with pMCAM, but less efficient than gene electrotransfer in in vivo tumor gene therapy due to the lack of antiangiogenic effect after silencing Mcam by magnetofection.

show abstract

Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Mcam Silencing With RNA Interference Using Magnetofection has Antitumor Effect in Murine Melanoma

Prosen

Markelc

Dolinšek

et al. 2014

Molecular Therapy - Nucleic Acids

View full text Add to dashboard Cite

show abstract

“…1 Until now, numerous potential applications of peroxidases have been investigated in the fields of biosensors, immunoassays, cell growth, and pollutant removal. [9][10][11][12][13] Hemin is the catalytic active center of peroxidase, and hemin-based composites was found to exhibited the enzyme-like activity unexpectedly and this function has since been investigated extensively. Recently, artificial enzymes that structure and function like the natural enzymes, have emerged as promising alternatives due to their highly stable and low-cost characteristics.…”

Section: Introductionmentioning

confidence: 99%

A biomimetic enzyme modified electrode for H₂O₂ highly sensitive detection

Kong

et al. 2015

Analyst

View full text Add to dashboard Cite

An efficient catalyst based on artificial bionic peroxidase was synthesized for electrocatalysis. A poly(ethyleneimine)/Au nanoparticle composite (PEI-AuNP) was prepared and it was then linked to hemin via a coupling reaction between carboxyl groups in hemin and amino groups in PEI without the activation of a carboxyl group by carbodiimide. Fourier transform infrared (FTIR) spectroscopy verified the formation of amido bonds within the structure. The presence of AuNPs contributed greatly in establishing the amido bonds within the composite. Transmission electron microscopy (TEM) and UV-visible spectroscopy were also used to characterize the PEI-AuNP-hemin catalyst. PEI-AuNP-hemin exhibited intrinsic peroxidase-like catalytic activities. The PEI-AuNP-hemin deposited on a glass carbon electrode had strong sensing for H2O2 with a well-defined linear relationship between the amperometric response and H2O2 concentration in the range from 1 μM to 0.25 mM. The detection limit was 0.247 nM with a high sensitivity of 0.347 mA mM(-1) cm(-2). The peroxidase-like catalytic activity of PEI-AuNP-hemin is discussed in relation to its microstructure. The study suggests that PEI-AuNP-hemin may have promising application prospects in biocatalysis and bioelectronics.

show abstract

“…Further, systemic administration to mice bearing T98G-derived flank xenografts resulted in almost imperceptible tumor growth and induced apoptosis in tumor tissue. To overcome treatment limitations of adenoid cystic carcinoma, the Fe 3 O 4 -PEI-plasmid complex (FPP) was generated, in which iron oxide NPs were modified by positively charged PEI to enable them to carry pACTERT-TRAIL [60]. The efficiency of FPP-mediated transfection was sixfold higher than that of PEI alone or of Lipo2000, and FPP-mediated TRAIL gene transfer efficiently inhibited SACC-83 tumor growth.…”

Section: Nonviral Vectorsmentioning

confidence: 99%

TRAIL-based gene delivery and therapeutic strategies

Zhong

Wang

et al. 2019

Acta Pharmacol Sin

View full text Add to dashboard Cite

TRAIL (tumor necrosis factor-related apoptosis-inducing ligand), also known as APO2L, belongs to the tumor necrosis factor family. By binding to the death receptor 4 (DR4) or DR5, TRAIL induces apoptosis of tumor cells without causing side toxicity in normal tissues. In recent years TRAIL-based therapy has attracted great attention for its promise of serving as a cancer drug candidate. However, the treatment efficacy of TRAIL protein was under expectation in the clinical trials because of the short half-life and the resistance of cancer cells. TRAIL gene transfection can produce a "bystander effect" of tumor cell killing and provide a potential solution to TRAIL-based cancer therapy. In this review we focus on TRAIL gene therapy and various design strategies of TRAIL DNA delivery including non-viral vectors and cell-based TRAIL therapy. In order to sensitize the tumor cells to TRAIL-induced apoptosis, combination therapy of TRAIL DNA with other drugs by the codelivery methods for yielding a synergistic antitumor efficacy is summarized. The opportunities and challenges of TRAIL-based gene delivery and therapy are discussed.

show abstract

Antitumor effect of human TRAIL on adenoid cystic carcinoma using magnetic nanoparticle–mediated gene expression

Cited by 25 publications

References 44 publications

Mcam Silencing With RNA Interference Using Magnetofection has Antitumor Effect in Murine Melanoma

Mcam Silencing With RNA Interference Using Magnetofection has Antitumor Effect in Murine Melanoma

A biomimetic enzyme modified electrode for H₂O₂ highly sensitive detection

TRAIL-based gene delivery and therapeutic strategies

Contact Info

Product

Resources

About

Antitumor effect of human TRAIL on adenoid cystic carcinoma using magnetic nanoparticle–mediated gene expression

Cited by 25 publications

References 44 publications

Mcam Silencing With RNA Interference Using Magnetofection has Antitumor Effect in Murine Melanoma

Mcam Silencing With RNA Interference Using Magnetofection has Antitumor Effect in Murine Melanoma

A biomimetic enzyme modified electrode for H2O2 highly sensitive detection

TRAIL-based gene delivery and therapeutic strategies

Contact Info

Product

Resources

About

A biomimetic enzyme modified electrode for H₂O₂ highly sensitive detection