Antitumor Effect of the Essential Oil from Leaves of Guatteria pogonopus (Annonaceae)

Abstract

Guatteria pogonopus Martius, a plant belonging to the Annonaceae family, is found in the remaining Brazilian Atlantic Forest. In this study, the chemical composition and antitumor effects of the essential oil isolated from leaves of G. pogonopus was investigated. The chemical composition of the oil was determined by GC-FID and GC/MS analyses. The in vitro cytotoxicity was evaluated against three different tumor cell lines (OVCAR-8, NCI-H358M, and PC-3M), and the in vivo antitumor activity was tested in mice bearing sarcoma 180 tumor. A total of 29 compounds was identified and quantified in the oil. The major compounds were γ-patchoulene (13.55%), (E)-caryophyllene (11.36%), β-pinene (10.37%), germacrene D (6.72%), bicyclogermacrene (5.97%), α-pinene (5.33%), and germacrene B (4.69%). The essential oil, but neither (E)-caryophyllene nor β-pinene, displayed in vitro cytotoxicity against all three tumor cell lines tested. The obtained average IC50 values ranged from 3.8 to 20.8 μg/ml. The lowest and highest values were obtained against the NCI-H358M and the OVCAR-8 cell lines, respectively. The in vivo tumor-growth-inhibition rates in the tumor-bearing mice treated with essential oil (50 and 100 mg/kg/d) were 25.3 and 42.6%, respectively. Hence, the essential oil showed significant in vitro and in vivo antitumor activity.

Essential Oil of the Plants Growing in the Brazilian Amazon: Chemical Composition, Antioxidants, and Biological Applications

“…The Annonaceae family is characterized by a pantropical family of trees, bushes, and climbers, and is found especially in tropical lowlands [ 92 ]. The family is characterized by species rich in essential oils with potential in vitro inhibitory activity against cancer cells [ 36 , 92 ]. This biological activity was observed for the essential oil from the leaves of Anaxagorea brevipes collected in Manaus, Amazonas, Brazil.…”

Chemical Characterization and in Vitro Antitumor Activity of the Essential Oils from the Leaves and Flowers of Callistemon viminalis

“…Considering that the melanoma is high lethal [3], the results obtained in the present study are very promising and further studies will be performed to verify whether the cytotoxic activity previously observed is related to pro-apoptotic potential of the essential oils. Reduction of cell viability in HT144 cultures may be related to the presence of 1,8 cineole, α-pinene and α-terpineol, but considering that essential oils have a complex chemical composition, it is possible that the reduction in viability was the result of synergistic action of the constituents found in the EOs [28]. The monoterpenes are considered to be the active compounds, and, therefore, additional studies should be conducted to further investigate the relationship of monoterpenes with antiproliferative or cytotoxic effects of EOs on melanoma cells.…”

Anti-Oral Pathogens of Tecoma stans (L.) and Cassia javanica (L.) Flower Volatile Oils in Comparison with Chlorhexidine in Accordance with Their Folk Medicinal Uses

“… Exp Experimental retention index ( RI ) using a series of n-alkanes (C10–C40) calculated under identical experimental conditions. Rep Reported retention index according to the National Institute of Standards and Technology (NIST) library and published literature data and calculated under identical experimental conditions as the references [40,41,42,43,44,45,46,47,48,49]. %R The relative percentage of individual volatile components according to the peak area and calculated from the GC (Gas chromatography) chromatogram; RT = retention time.…”