Antitumor Effects of a Recombinant Baculovirus Displaying Anti-HER2 Scfv Expressing Apoptin in HER2 Positive SK-BR-3 Breast Cancer Cells

Meysami, Parisa; Rezaei, Fatemeh; Marashi, Sayed Mahdi; Amiri, Mohammad Mehdi; Bakker, Emyr; Mokhtari‐Azad, Talat

doi:10.2217/fvl-2018-0187

Cited by 7 publications

(3 citation statements)

References 41 publications

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“…Recent researches have demonstrated that the negative feedback of Survivin was able to increase the apoptotic cells significantly when applied along with apoptin compared with treatment alone. 27 Another study reported that the integrated application of apoptin with interleukin-18 led to a severe Th1 immune response to Lewis lung carcinomas which resulted in significant growth inhibition of these tumor cells. 28 The most critical challenge in studies of apoptin is related to the development of a technique for efficient apoptin delivery to the tumor lesion site.…”

Section: Discussionmentioning

confidence: 99%

Antiproliferative Effects of Recombinant Apoptin on Lung and Breast Cancer Cell Lines

et al. 2020

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Background: Selective therapy has always been the main challenge in cancer treatments. Various non-replicative oncolytic viral systems have revealed the safety and efficacy of using viruses and these products. The aim of this paper is to examine the impact of recombinant apoptin on the proliferation of lung cancer and breast cancer cell lines. Methods: The present study consisted of two steps of expression of recombinant apoptin and its anti-proliferative effects on normal and cancer cells. In the first step, following bioinformatics and optimizing apoptin gene sequencing and synthesis, it was expressed using vector PET28a and E. coli BL21 (DE3). The expressed recombinant apoptin was confirmed by analytical SDSPAGE and then purified using Ni affinity chromatography. In the second step, the antiproliferative effects of recombinant apoptin on lung cancer, breast cancer and primary cell lines were determined using MTT assay. Results: According to the results of SDS-PAGE gel assay, recombinant apoptin was visible in the 14 kDa band. Also, the MTT assay results indicated that the antiproliferative effects of recombinant apoptin in cancer cell lines was different compared with the primary cell line, and followed a dose-dependent manner in both cell lines. The highest cytotoxicity (lowest cell viability) groups were 0.2 mg/mL in lung cancer (0.32 ± 0.015) (P<0.001), and in breast cancer (0.33 ± 0.031) (P<0.001) and 0.032 mg/mL in primary cells (0.17 ± 0.004) (P<0.01), as compared to the control groups. Conclusion: Our results confirmed that recombinant apoptin can induce antiproliferative effects in lung cancer and breast cancer cell lines, but not in normal monkey kidney cell line Vero; thus, it can be introduced as a promising novel specific antitumor agent after further evaluation in clinical trials.

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Section: Discussionmentioning

confidence: 99%

Antiproliferative Effects of Recombinant Apoptin on Lung and Breast Cancer Cell Lines

et al. 2020

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“… 100 In comparison, rBVs displaying human epidermal growth factor-2 (HER2) single-chain variable domain fragments (scFV) while expressing Apoptin bind specifically HER2 positive SK-BR-3 breast cancer cells reducing cancer cell viability. 101 Similarly, a rBV expressing BIMs, a strong apoptosis inducer, resultedin selective death of HCV-positive cells only further proving BV’s potential for selective gene therapy. 102 The selective treatment of an individual’s malfunctioning or impaired cells can mitigate the systemic and adverse effects seen in traditional medical treatments, significantly improving the quality of treatment, care, and life.…”

Section: Application Of Baculoviruses In Gene and Other Therapiesmentioning

confidence: 99%

Baculoviruses in Gene Therapy and Personalized Medicine

Schaly¹,

Ghebretatios²,

Prakash³

2021

BTT

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This review will outline the role of baculoviruses in gene therapy and future potential in personalized medicine. Baculoviruses are a safe, non-toxic, non-integrative vector with a large cloning capacity. Baculoviruses are also a highly adaptable, low-cost vector with a broad tissue and host tropism due to their ability to infect both quiescent and proliferating cells. Moreover, they only replicate in insect cells, not mammalian cells, improving their biosafety. The beneficial properties of baculoviruses make it an attractive option for gene delivery. The use of baculoviruses in gene therapy has advanced significantly, contributing to vaccine production, anti-cancer therapies and regenerative medicine. Currently, baculoviruses are primarily used for recombinant protein production and vaccines. This review will also discuss methods to optimize baculoviruses protein production and mammalian cell entry, limitations and potential for gene therapy and personalized medicine. Limitations such as transient gene expression, complement activation and virus fragility are discussed in details as they can be overcome through further genetic modifications and other methods. This review concludes that baculoviruses are an excllent candidate for gene therapy, personalized medicine and other biotherapeutic applications.

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“…The expression of anti-HER2 allows the selective binding of baculovirus to tumor cells, since HER2 is a transmembrane receptor tyrosine kinase that is overexpressed in 30% of all cases of breast cancer and aggressive tumors. Once the baculovirus specifically enters cancer cells, apoptonin expression begins leading to tumor cell death [ 61 ].…”

Section: Introductionmentioning

confidence: 99%

Baculovirus Display of Peptides and Proteins for Medical Applications

Rodríguez-Hernández

Martínez-Flores

Cruz-Reséndiz

et al. 2023

Viruses

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Baculoviridae is a large family of arthropod-infective viruses. Recombinant baculoviruses have many applications, the best known is as a system for large scale protein production in combination with insect cell cultures. More recently recombinant baculoviruses have been utilized for the display of proteins of interest with applications in medicine. In the present review we analyze the different strategies for the display of proteins and peptides on the surface of recombinant baculoviruses and provide some examples of the different proteins displayed. We analyze briefly the commercially available systems for recombinant baculovirus production and display and discuss the future of this emerging and powerful technology.

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Antitumor Effects of a Recombinant Baculovirus Displaying Anti-HER2 Scfv Expressing Apoptin in HER2 Positive SK-BR-3 Breast Cancer Cells

Cited by 7 publications

References 41 publications

Antiproliferative Effects of Recombinant Apoptin on Lung and Breast Cancer Cell Lines

Antiproliferative Effects of Recombinant Apoptin on Lung and Breast Cancer Cell Lines

Baculoviruses in Gene Therapy and Personalized Medicine

Baculovirus Display of Peptides and Proteins for Medical Applications

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