Antitumor effects of Bulbus Fritillariae cirrhosae on Lewis lung carcinoma cells in vitro and in vivo

Wang, Dongdong; Wang, Shu; Zhang, Li; Zu, Li; Ma, Jing; Luo, Yuanqing; Xiao, Wei

doi:10.1016/j.indcrop.2013.12.054

Cited by 34 publications

(37 citation statements)

References 30 publications

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“…To date, there are more than 100 types of cancer, but the common cancers are lung, breast, colorectal and stomach [2]. Surgery, chemotherapy and radiation are commonly used for cancer treatment along with side effects [3]. Over the past decades, natural products have attracted a great deal of attention due to their utilization of cancer prevention and treatment.…”

Section: Introductionmentioning

confidence: 99%

A Comprehensive Assessment of Bioactive Metabolites, Antioxidant and Antiproliferative Activities of Cyclocarya paliurus (Batal.) Iljinskaja Leaves

Zhou

Chen

Lin

et al. 2019

Forests

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Cyclocarya paliurus (Batal.) Iljinskaja is an indigenous and multifunction tree species in China, but it is mainly used in pharmaceutical and nutraceutical ingredients. To make a comprehensive evaluation on its bioactive metabolites, antioxidant and antitumor potentials of C. paliurus leaves, the leaf samples were collected from 15 geographic locations (natural populations) throughout its distribution areas. High-performance liquid chromatography (HPLC) and colorimetric methods were used to detect the contents of bioactive metabolites. The antioxidant activity was evaluated by 2,2′-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and reducing power assays. The antiproliferative activity on different cancer cell types was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Contents of bioactive metabolites, and antioxidant and antiproliferative activities in the extracts were significantly affected by solvent and population. In most cases, the contents of flavonoids and triterpenoids, and the antioxidant and antiproliferative activities in the ethanol extracts were higher than the water extracts. The best scavenging capacity of DPPH (IC50 = 0.34 mg/mL) and ABTS (IC50 = 0.50 mg/mL) radical occurred in the ethanol extracts of S15 and S7 population respectively, while the strongest reducing power (EC50 = 0.71 mg/mL) was achieved in the ethanol extracts of S14 population. The antiproliferation effects of C. paliurus extracts on cancer cells varied with different cell types. The HeLa cell was the most sensitive to C. paliurus extracts, and their IC50 values of the ethanol extracts varied from 0.13 to 0.42 mg/mL among C. paliurus populations. Redundancy analysis showed that total polyphenol had the greatest contribution to the antioxidant activity, but total flavonoid was mostly responsible for the antiproliferation effects. These results would provide important scientific evidences not only for developing C. paliurus as a potent antioxidant and antitumor reagent, but also for obtaining the higher yield of bioactive compounds in the C. paliurus plantation.

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Section: Introductionmentioning

confidence: 99%

A Comprehensive Assessment of Bioactive Metabolites, Antioxidant and Antiproliferative Activities of Cyclocarya paliurus (Batal.) Iljinskaja Leaves

Zhou

Chen

Lin

et al. 2019

Forests

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“…The different extracts of BFU were prepared according to previously described methods [ 14 ]. Briefly, the dried powder was immersed in ammonia, and then extracted with EtOH solvent by maceration for 6 hours.…”

Section: Methodsmentioning

confidence: 99%

“…From the previous research, we could conclude that the CE had more total alkaloids [ 13 , 14 ]. The CE which mainly contained total alkaloids showed the highest antitumor activity in vitro among three extracts (CE, HE and WE).…”

Section: Methodsmentioning

confidence: 99%

“…The absorbance was recorded on a Thermo Scientific microplate spectrophotometer (Thermo Fisher Scientific Inc., USA) at the wavelength of 490 nm. The concentration of the extracts which gives 50% growth inhibition is referred to as the IC50 (μg/ml), which was calculated for each extracts from the dose-response curves [ 13 , 14 ].…”

Section: Methodsmentioning

confidence: 99%

“…Besides, the crude alkaloid extract of bulbs of Fritillaria puqiensis and puqietinone could inhibit the growth of three types of cancers [ 12 ]. Moreover, our previous studies also suggested that the crude alkaloid extract of bulbs of Fritillaria Cirrhosae showed remarkable antitumor in vitro and in vivo [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of antitumor property of extracts and steroidal alkaloids from the cultivated Bulbus Fritillariae ussuriensis and preliminary investigation of its mechanism of action

Wang

Jiang

et al. 2015

BMC Complement Altern Med

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BackgroundCancer is well known as a leading cause of death in the world. At present, it is the very active area to search for anticancer drugs from natural products. In this study, we evaluated the antitumor property of chloroform extract (CE), n-hexane extract (HE), water extract (WE) and steroidal alkaloids from the cultivated Bulbus Fritillariae ussuriensis (BFU) and its preliminary mechanism for its action was investigated.MethodsFirstly, cytotoxicity of the different extracts from BFU against Lewis lung carcinoma cell line (LLC), Human ovarian cancer cell line (A2780), human hepatocellular carcinoma cell line (HepG2), human lung carcinoma cell line (A549) was measured by MTT assay. Then, we identified the compounds from the active extract of BFU by bioassay guided isolation, determined their antitumor activity in vitro, and detected cell cycle distribution using flow cytometry. Moreover, the extract of BFU which showed remarked anti-proliferative activity in vitro was further evaluated using S180 and LLC tumor models. Additionally, a preliminary investigation of the mechanism of the action was carried out by using histological and immunohistochemical staining technique.ResultsThe results showed that CE and the purified total alkaloids of BFU (TAFU) exhibited stronger cytotoxic activity than the others (WE and HE). We further isolated the four main steroidal alkaloids from TAFU, and found all alkaloids showed significant cytotoxicity, and peimisine induced G0/G1 phase arrest and increased apoptosis. The results showed that TAFU had significant antitumor activity and low toxicity in vivo. Additionally, the immunohistochemical examinations signified that TAFU remarkably increased caspase-3 expression and reduced microvessel density (MVD) in tumor tissues of transplantable S180 and LLC tumor models.ConclusionsThese results achieved suggested that the steroidal alkaloids could hold a good potential for use as an antitumor drug. Notably, our finding is the first report on the antitumor activity of extracts and steroidal alkaloids from the cultivated BFU in vitro and in vivo and its mechanisms.

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Fritillaria steroidal alkaloids and their multi-target therapeutic mechanisms: insights from network pharmacology

Kumar,

Sharma

et al. 2024

Naunyn-Schmiedeberg's Arch Pharmacol

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Antitumor effects of Bulbus Fritillariae cirrhosae on Lewis lung carcinoma cells in vitro and in vivo

Cited by 34 publications

References 30 publications

A Comprehensive Assessment of Bioactive Metabolites, Antioxidant and Antiproliferative Activities of Cyclocarya paliurus (Batal.) Iljinskaja Leaves

A Comprehensive Assessment of Bioactive Metabolites, Antioxidant and Antiproliferative Activities of Cyclocarya paliurus (Batal.) Iljinskaja Leaves

Evaluation of antitumor property of extracts and steroidal alkaloids from the cultivated Bulbus Fritillariae ussuriensis and preliminary investigation of its mechanism of action

Fritillaria steroidal alkaloids and their multi-target therapeutic mechanisms: insights from network pharmacology

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