Antitumor Effects of Turmeric on OVCAR-3 Ovarian Cancer Cell Lines

Abstract

Introduction: Ovarian cancer is the deadliest gynecological malignancy, usually not detected until the late stages. In vitro cell culture is a method used to study the behavior of cells in a controlled environment. Turmeric has attracted the attention of scientists due to its anticancer potential. Method: OVCAR-3 cells were cultured in RPMI medium with 100 units/mL-100 μg/mL of penicillin-streptomycin and 10% foetal bovine serum in a CO2 incubator. Turmeric extract was diluted in DMSO. Different concentrations of turmeric extract were prepared. Annexin-V staining was performed to test the translocation of phosphatidylserine to the outer side of the cell membrane as a clear indicator of apoptosis. Results: Turmeric extract significantly reduced the viability of OVCAR-3 cells both within 24 and 48 hours of exposure. OVCAR-3 cells were treated with IC50 concentration of turmeric extract for 24 hours. 82.60% of cells were viable. The percentages of the dead, early apoptotic, and late apoptotic cells were detected to be 0.80%, 9.70%, and 6.90%, respectively. Untreated OVCAR-3 cells had migration ability. OVCAR-3 cells exposed to an IC50 concentration of turmeric extract for 24 hours did not close the scratch area. Conclusion: In this research, anticancer effects of turmeric have been demonstrated by different analysis methods.

The ameliorative effect of turmeric (Curcuma longa Linn) extract and its major constituent, curcumin, and its analogs on ethanol toxicity

“…Turmeric is used in traditional medicine to treat liver and respiratory disorders, allergies, sinusitis, and anorexia (Razavi et al, 2021). At present, this herb is also known to have antirheumatic (Nakisa & Rahbardar, 2021), anti‐cancer (Gurer et al, 2022), antidotal (Ara et al, 2020; Hosseini & Hosseinzadeh, 2018), anti‐hypertriglyceridemia (Mollazadeh et al, 2019), hypoglycemic (El‐Hadary & Sitohy, 2021), anti‐depressive (Alavi et al, 2021), hepatoprotective, anti‐obesity (Vafaeipour et al, 2022), cardioprotective (Akhter & Nahar, 2022), and anti‐microbial (Soleimani et al, 2018) effects. Additionally, turmeric has been shown to have anti‐inflammatory (Liu et al, 2022), antioxidant (Adab et al, 2019), and anti‐apoptotic (Ibrahim et al, 2019) effects in numerous studies.…”