Antitumor Promoting Effect of an Active Component of Polyporus, Ergosterol and Related Compounds on Rat Urinary Bladder Carcinogenesis in a Short-Term Test with Concanavalin A.

Abstract

The effect of fractions from a water extract of Polyporus on bladder tumor promotion was examined using 5% sodium saccharin (SS) in a short-term test with concanavalin A (Con A) in Wistar rats. Rats were given N-butyl-N-(4-hydroxybutyl) nitrosamine (BHBN) in drinking water for one week, and then promoter alone or test samples (given orally) plus promoter was administered for 3 weeks. Treatment with the BuOH fraction isolated from the water extract showed a strong inhibitory effect against the promoter. It was found that the inhibitory effect of the BuOH fraction is due to the effect of ergosterol contained in the fraction. Treatment with ergosterol showed a strong inhibitory effect against 5% SS, 0.01% BHBN, 3% DL-tryptophan (Trp) or 2% butylated hydroxyanisole (BHA); ID50 was 1.4 microg/kg/d, 2.9 microg/kg/d, 11.6 microg/kg/d, and 11.7 microg/kg/d against SS, BHBN, Trp and BHA, respectively. We also examined the effect of steroids and related compounds. Squalene and vitamin D2 showed strong inhibitory effect against 5% SS-induced bladder tumor promotion. These results strongly suggest that ergosterol could provide significant protection against the promotion of bladder tumor induced by many types of promoters in the environment.

Human Cytochrome P450scc (CYP11A1) Catalyzes Epoxide Formation with Ergosterol

“…Ergosterol taken up by the gut may act as a membrane antioxidant (Wiseman, 1993). Ergosterol can modify the effect of cholesterol on human cell cycle progression (Suárez et al, 2002) and has antitumor effects in cell culture (Yazawa et al, 2000) and in vivo in rats (Mitani et al, 2004).…”

Development of a Novel Methodology for the Analysis of Ergosterol in Mushrooms

“…In fact, the health-promoting properties of ergosterol, including the 13 antioxidant (Shao et al 2010), anti-inflammatory (Kuo et al 2011) antihyperlipidemic (Hu et al 2006), genetic expression control (Lochman and Mikes 2005) and antitumoral activities (Takaku et al 2001;Yazawa et al 2000), are closely related to the uptake doses. Furthermore, it will be important to understand how its concentration varies during the main fungi development stages.…”

Gas chromatography-mass spectroscopic (GC-MS) analysis of n-Hexane extract of <i>Lentinus tuber-regium</i> (Fr) Fr (Polyporaceae) syn <i>Pleurotus tuber regium</i> Fr sclerotia

“…The occurrence of ergosterol derivatives (17.91 %) in the n-hexane extract as the predominant steroids could be due to the reported occurrence of ergosterols in yeast and fungal cell membranes functioning in same manner cholesterol does in animal cells. Research has shown ergosterol may exhibit some degree of antitumor properties [17,18]. Ergosterols are known to act as biological precursors of vitamin D2 hence can be classified as provitamins [19].…”