Antitumor Properties ofGymnosperma GlutinosumLeaf Extracts

Gómez-Flores, Ricardo; Verástegui-Rodríguez, L; Quintanilla-Licea, Ramiro; Tamez‐Guerra, Patricia; Monreal-Cuevas, Enriqueta; Taméz-Guerra, Reyes; Rodríguez‐Padilla, Cristina

doi:10.1080/07357900802192190

Cited by 15 publications

(24 citation statements)

References 24 publications

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“…Previous studies reported that P. nigrum L. extract and piperine possess antitumor [17], anti-inflammatory [7] and immunomodulating potential [7,17,18]. Pathak and Khandelwal [6] reported anti-oxidative, anti-apoptotic and chemo-protective ability of piperine in blastogenesis, cytokine resease and restoration of spleen cells population, and they recommended the therapeutic usefulness of piperine in immuno-compromised situations.…”

Section: Resultsmentioning

confidence: 99%

Induction of Humoral Immunity Through the Enhancement of IgM Production in Murine Splenic Cells by Ethanolic Extract of Seed of Piper nigrum L.

Sarker

2012

J. Sci. Res.

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The immunostimulating activity of 70% ethanololic extract of P. nigrum L. was investigated by measuring the production of immunoglobulin M (IgM) and the proliferation of murine splenic cells in vitro. The production of IgM in cultured supernatants was determined by an enzyme-linked immunosorbent assay (ELISA) and proliferation of cells was measured by 3-(4,5-dimethylthiazol-2-y)-2,5-diphenylterazolium bromide (MTT) assay. The extract at the doses of 0.01 and 0.1 mg/ml significantly augmented the production of polyclonal IgM production (0.3206 and 0.5014 µg/ml, respectively) compared to control (0.1465 µg/ml). No dose of the extract could remarkably increase the proliferation of splenocytes. Thus, the present study clearly demonstrates the immunostimulating activity of ethanololic extract of Piper nigrum; exhibited mainly by the differentiation of B cells to plasma cells rather than proliferation of splenocytes.

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Section: Resultsmentioning

confidence: 99%

Induction of Humoral Immunity Through the Enhancement of IgM Production in Murine Splenic Cells by Ethanolic Extract of Seed of Piper nigrum L.

Sarker

2012

J. Sci. Res.

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“…One hundred microliters of the cell suspensions were then added to flat-bottomed 96-well plates (Becton Dickinson, Cockeysville, MD), containing 100 µl triplicate cultures of complete RPMI 1640 or DMEM media (unstimulated controls), the extracts at various concentrations (extracts were dissolved in complete RPMI 1640 (for L5178Y-R cells) or DMEM medium (for B16F10 cells), extract-free vehicles (vehicles were similarly processed as with P. marginatus extracts, but without plant material), and vincristine as a positive control. After incubation for 44 h at 37°C with 5% CO2, MTT (0.5 mg ml -1 , final concentration) was added, and cultures were additionally incubated for 4 h. Next, cell cultures were incubated for 16 h with extraction buffer (100 µl well -1 ) and optical densities, resulting from dissolved formazan crystals, were then read in a microplate reader (DTX 880 Multimode detector, Becton Dickinson, Austria) at 570 nm (Gomez-Flores et al, 2009) …”

Section: L5178y-r and B16f10 Cells Preparation And Culturementioning

confidence: 99%

“…Thymus and spleen were removed immediately after mouse death, and a single cell-suspension was prepared by disrupting the organs in RPMI 1640 medium as previously reported (Gomez-Flores et al, 2009). The cell suspensions were washed three times in this medium, suspended, and adjusted to 1 × 10 7 cells ml -1 in complete RPMI 1640 medium.…”

Section: Cell Preparation and Culturementioning

confidence: 99%

“…T cell proliferation was determined by a colorimetric technique using MTT (Gomez-Flores et al, 2009). Thymus and spleen cell suspensions (100 µl of 1 × 10 7 cells ml -1 ) were added to flatbottomed 96-well plates (Becton Dickinson) containing triplicate cultures (100 µl) of RPMI 1640 medium supplemented with 5% fetal bovine serum (unstimulated control), Con A (2.5 µg ml -1 ), and plant extracts at various concentrations for 48 h at 37°C in 95% air-5% CO2 atmosphere.…”

Section: T Cell Proliferation Assaymentioning

confidence: 99%

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Antitumor activity of Pachycereus marginatus (DC.) Britton Rose extracts against murine lymphoma L5178Y-R and skin melanoma B16F10 cells

Hernandez-Martinez¹,

Gómez-Flores²,

Tamez‐Guerra³

et al. 2016

J. Med. Plants Res.

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Pachycereus marginatus (DC.) Britton & Rose is a species belonging to the family Cactaceae. In traditional medicine, it is recommended to treat diabetes and gastrointestinal infections; however, there are no studies related to its use in cancer treatment. The in vitro antitumor effect of P. marginatus hexane, chloroform, methanol, and methanol-aqueous partition stem extracts, against murine lymphoma L5178Y-R and skin melanoma B16F10 cells, was evaluated in liquid medium by the colorimetric 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) reduction assay. The extracts resulted in up to 84, 85, 84, and 82% cytotoxicity (p < 0.05) to L5178Y-R cells, respectively, and up to 39, 51, 48, and 42% cytotoxicity (p < 0.05) to B16F10 cells, respectively. Vehicle controls were not cytotoxic for tumor cells, and along with the extracts they did not affect viability of resident murine thymus and spleen lymphocytes. Taken together, the present results showed that P. marginatus extracts possess antitumor potential against L5178Y-R lymphoma and B16F10 skin melanoma cells.

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“…Plant metabolites are known to have direct positive effects in the treatment and management of infectious diseases and cancer. In addition, the indirect effects of plant metabolites through immunomodulation are well studied 6 .…”

Section: Introductionmentioning

confidence: 99%

Untitled

2013

IJPSR

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ABSTRACT:Aim: To compare the immunomodulatory effect of Ocimum sanctum and Ocimum basilicum in mice. Methods: Acute oral toxicity test was performed as per OECD -23 guidelines. Maximum dose level of 2000 mg/kg was selected for toxicological evaluation. Delayed type hypersensitivity reaction were carried out for assessment of cell mediated immune functions Result: Administration of Ocimum sanctum and Ocimum basilicum did not produced any mortality or any signs of behavioral changes or toxicity at the dose level of 2000 mg/kg body weight in mice. The result of the present study showed that acute administration of extract of Ocimum sanctum and Ocimum basilicum may be safe as the LD50 may be greater than 2000mg/kg. Treatment with Ocimum sanctum enhanced DTH reaction, which is reflected from foot pad thickness when compared with Ocimum basilicum and control groups whereas a similar effect was observed when compared to the reference drug Aswagandha choorna, which is a well-known immune booster. Conclusion: Increase in DTH reaction in mice in response in T cell dependent antigen revealed the stimulatory effect of Ocimum sanctum on T cells.

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Antitumor Properties ofGymnosperma GlutinosumLeaf Extracts

Cited by 15 publications

References 24 publications

Induction of Humoral Immunity Through the Enhancement of IgM Production in Murine Splenic Cells by Ethanolic Extract of Seed of <i>Piper nigrum</i> L.

Induction of Humoral Immunity Through the Enhancement of IgM Production in Murine Splenic Cells by Ethanolic Extract of Seed of <i>Piper nigrum</i> L.

Antitumor activity of Pachycereus marginatus (DC.) Britton Rose extracts against murine lymphoma L5178Y-R and skin melanoma B16F10 cells

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