Antitumoral and antimetastatic effect of antiangiogenic plasmids in B16 melanoma: Higher efficiency of the recombinant disintegrin domain of ADAM 15

Daugimont, Liévin; Vandermeulen, Gaëlle; Defresne, Florence; Bouzin, Caroline; Mir, Lluis M.; Bouquet, Céline; Feron, Olivier; Préat, Véronique

doi:10.1016/j.ejpb.2011.02.001

Cited by 24 publications

(13 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The study demonstrated good antitumor effectiveness after intramuscular GET in the MDA-MB-231 breast tumor model and the anti-metastatic potential on B16F10 murine melanoma model in mice [2]. Another study also in melanoma B16F10 tumor model showed the antitumor and antimetastatic effectiveness of a plasmid coding for RDD after intratumoral transfection [8]. All those results were also confirmed in our previous studies.…”

Section: Discussionsupporting

confidence: 79%

Antimetastatic Potential in Mice after Gene Therapy with Plasmid AMEP

et al. 2016

View full text Add to dashboard Cite

Abstract-Gene electrotransfer of Plasmid AMEP is a new antiangiogenic approach in cancer treatment. While the local effects after intratumoral administration have been already studied, systemic effects still need to be elucidated. Therefore intramtumoral or intramuscular gene therapy with Plasmid AMEP was performed and effects on distant tumors or metastases were observed and compared. Only 8% of the mice developed distant metastases after Plasmid AMEP gene electrotransfer, compared to 40% in the control groups. Additionally, intramuscular gene elctrotransfer performed before the tumor implantation, has no effect on the tumor outgrowth regardless of the GET protocol used. Study showed that gene therapy with Plasmid AMEP has antimetastatic potential only after intratumoral, but not after intramuscular gene electrotransfer. Most probably circulating AMEP peptide produced in muscle cells may be rapidly biodegraded and could not be able to reach tumor site in high amounts needed for AMEP effectiveness.

show abstract

Section: Discussionsupporting

confidence: 79%

Antimetastatic Potential in Mice after Gene Therapy with Plasmid AMEP

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Electroporation is a non-viral delivery method based on membrane destabilization and DNA electrophoresis, mediated by electric pulses. Efficient delivery of plasmid into muscle, skin, tumor and other tissues using in vivo electroporation has thus been demonstrated [4][5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

The site of administration influences both the type and the magnitude of the immune response induced by DNA vaccine electroporation

Vandermeulen

Vanvarenberg

Beuckelaer

et al. 2015

Vaccine

Self Cite

View full text Add to dashboard Cite

a b s t r a c tWe investigated the influence of the site of administration of DNA vaccine on the induced immune response. DNA vaccines were administered by electroporation at three different sites: tibial cranial muscle, abdominal skin and ear pinna. Aiming to draw general conclusions about DNA vaccine delivery, we successively used several plasmids encoding either luciferase and ovalbumin as models or gp160 and P1A as vaccines against HIV and P815 mastocytoma, respectively. Low levels and duration of luciferase transgene expression were observed after electroporation of the abdominal skin, partly explaining its lower immunogenic performance as compared to the other sites of administration. Analyses of OT-I CD8+ and OT-II CD4+ T cell responses highlighted the differential impact of the delivery site on the elicited immune response. Muscle electroporation induced the strongest humoral immune response and both muscle and ear pinna sites induced cellular immunity against gp160. Ear pinna delivery generated the highest level of CTL responses against P1A but electroporation of muscle and ear pinna were equally efficient in delaying P815 growth and improving mice survival. The present study demonstrated that the site of administration is a key factor to be tested in the development of DNA vaccine.

show abstract

“…On the other hand, previous reports demonstrated that addition of recombinant ADAM15 d.d. inhibited the tumor growth and metastasis of B16F10 murine melanoma cells and MDA-MB-231 human breast cancer cells in vivo (Daugimont et al, 2011;Trochon-Joseph et al, 2004), indicating that interaction of ADAM15 and integrins via d.d. plays a critical role in tumor cell growth and metastasis.…”

Section: Discussionmentioning

confidence: 99%

“…The ADAM15 molecule consists of various domains with proteinase and integrin binding activities (Daugimont et al, 2011;Hart et al, 2005;Maretzky et al, 2009;Najy et al, 2008b;Ohtsu et al, 2006;TrochonJoseph et al, 2004;Wu et al, 2008;Zhong et al, 2008). One of the reason for exhibiting complex functions of ADAM15 might be due to its ability to interact with various integrins, leading to the distinct intracellular signaling.…”

Section: Discussionmentioning

confidence: 99%

The Cell to Cell Interaction of Breast Cancer Cells Regulates Cancer Invasion via Adam15

Ota

Ikesue

Matsui

et al. 2012

American Journal of Immunology

View full text Add to dashboard Cite

Increasing evidence suggests that a disintegrin and metalloproteinase 15 (ADAM15) have essential roles in the process of cancer metastasis via degradation of the extracellular matrix and binding to integrins. Among them, ADAM15 possesses an Arg-Gly-Asp (RGD) sequence within its disintegrin domain (d.d., hereafter) and binds to RGD recognizing-integrins such as αvβ3 and α5β1 and also interacts with integrin α9β1 in RGD-independent manner. Although these integrins play important roles in the process of cancer metastasis, the role of the interactions between ADAM15 and integrins during processes of cancer metastasis remains to be elucidated. We produced the specific antibody (8F7) that interferes with the interaction of human ADAM15 and integrin receptors and performed in vitro aggregation assay, invasion assay, proliferation assay, proteinase activity assay and cell-cell adhesion assay. 8F7 inhibited tumor cell aggregation, invasion and migration, but not proliferation of breast cancer cells and proteinase activity of ADAM15. Furthermore, the interactions between ADAM15 and integrin receptors induced collective cell migration, phosphorylation of Akt, which was known to promote invasion of breast cancer cells. These data suggested that the binding of ADAM15 to αvβ3 or α9β1 integrins through its d.d. Induces cell aggregation, migration and invasion of human breast cancer cells with concomitant activation of Akt signaling pathway.

show abstract

Antitumoral and antimetastatic effect of antiangiogenic plasmids in B16 melanoma: Higher efficiency of the recombinant disintegrin domain of ADAM 15

Cited by 24 publications

References 29 publications

Antimetastatic Potential in Mice after Gene Therapy with Plasmid AMEP

Antimetastatic Potential in Mice after Gene Therapy with Plasmid AMEP

The site of administration influences both the type and the magnitude of the immune response induced by DNA vaccine electroporation

The Cell to Cell Interaction of Breast Cancer Cells Regulates Cancer Invasion via Adam15

Contact Info

Product

Resources

About