Antitumoral potency of methanolic extract from Nitraria retusa leaves via its immunomodulatory effect

Abstract

BackgroundThe purpose of this study was to assess the antitumoral effect of the methanol extract (MeOH) from Nitraria retusa leaves and to investigate its immunomodulatory activity that mediated the prevention of tumor progression in tumor-bearing mice.MethodsBalb/c mice weighing 18–20 g were subcutaneously implanted with B16-F10 cells then injected intra-peritoneally, 7 days later with (200 mg/kg bw) of MeOH extract, for 21 days. After euthanization on day 21, the tumors were weighed. Lymphocyte proliferation, cytotoxic T lymphocyte (CTL) and NK activity were evaluated using the MTT assay. Macrophage phagocytosis was studied by measuring their lysosomal activity and nitric oxide production.ResultsThe methanol extract inhibited significantly the growth of the implanted tumor, and increased remarkably splenocyte proliferation as well as NK and CTL activities, in tumor-bearing mice. It also promoted lysosomal activity of treated animal macrophages.ConclusionOur findings suggest that antitumoral effect of MeOH extract is related with to immunomodulatory activity.

Antioxidant capacity of Nitraria retusa leaf extracts against mitomycin C-induced genetic toxicity in male mice

“…They suggested that the protective action of N. retusa fruit extract could result from its content of the phenolic compounds. Boubaker et al (2018) indicated that treatment of tumor-induced mice with leaf extracts of N. retusa showed anti-tumor activity which was attributed to its most important components in the N. retusa, namely, palmitic acid and β-sitosterols (Boubaker, Chaabane, et al, 2015a;Boubaker, Mokdad Bzeouich, et al, 2015b).…”

T cells regulation modulated by Sambucus javanica extracts in DMBA-exposed mice

“…The successive methanol extract of Ficus benghalensis has been known to have immunostimulant activity in lymphocyte proliferation such as T cells and B cells (26). Another in vivo experiment in tumor-bearing mice proved the methanol extract of Nitraria retusa leaves significantly promoted splenocyte proliferation and boosted the activity of natural killer cells and cytotoxic T lymphocytes (27). Similarly, in vitro and in vivo experiments revealed that Mesembryanthemum crystallinum extract was able to enhance human B and T cell lines proliferation (28).…”