Antitumoural effect of Synadenium grantii Hook f. (Euphorbiaceae) latex

Oliveira, Thais Latansio de; Munhoz, Antônio Carlos Mattar; Lemes, Bruna Mikulis; Minozzo, Bruno Rodrigo; Nepel, Angelita; Barison, Andersson; Fávero, Giovani Marino; Campagnoli, Eduardo Bauml; Beltrame, Flávio Luís

doi:10.1016/j.jep.2013.08.033

Cited by 31 publications

(29 citation statements)

References 35 publications

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“…The inhibition assay of hemagglutinating activity with several carbohydrates showed that lectins have higher specificity for D-galactose than for α-lactose since the minimum inhibitory concentration of D-galactose was lower than that of α-Lactose (Table 1). These potentially harmful substances, responsible for the observed cytotoxicity, could be decreased or completely removed after fractionating (de Oliveira et al, 2013; Adebayo et al, 2015). Protein fractions of E. tirucalli latex obtained from ion exchange chromatography showed hemagglutinating activity and were able to interact with several carbohydrates such as α-lactose, N-acetyl-D-galactosamine, N-acetyl-Dglucosamine, and D-mannose (Santana et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

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Eutirucallin: A Lectin with Antitumor and Antimicrobial Properties

Palharini

Richter

Silva

et al. 2017

Front. Cell. Infect. Microbiol.

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Eutirucallin is a lectin isolated from the latex of Euphorbia tirucalli, a plant known for its medical properties. The present study explores various characteristics of Eutirucallin including stability, cytotoxicity against tumor cells, antimicrobial and antiparasitic activities. Eutirucallin was stable from 2 to 40 days at 4°C, maintained hemagglutinating activity within a restricted range, and showed optimal activity at pH 7.0–8.0. Eutirucallin presented antiproliferative activity for HeLa, PC3, MDA-MB-231, and MCF-7 tumor cells but was not cytotoxic for non-tumorigenic cells such as macrophages and fibroblasts. Eutirucallin inhibited the Ehrlich ascites carcinoma in vivo and it was also observed that Eutirucallin inhibited 62.5% of Escherichia coli growth. Also, Eutirucallin showed to be effective when tested directly against Toxoplasma gondii infection in vitro. Therefore, this study sheds perspectives for pharmacological applications of Eutirucallin.

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Section: Resultsmentioning

confidence: 99%

“…Recent studies with species of the genus Euphorbia (family Euphorbeaceae) have revealed the presence of lectins, glycoproteins and enzymes with antitumoral and antiangiogenic activities (Rajesh et al, 2006; Nogueira et al, 2008; Oliveira et al, 2013). …”

Section: Introductionmentioning

confidence: 99%

Eutirucallin: A Lectin with Antitumor and Antimicrobial Properties

Palharini

Richter

Silva

et al. 2017

Front. Cell. Infect. Microbiol.

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“…Phytochemical analysis using TLC, together with standard compounds already isolated from the plant (friedelin, 3b-friedelinol, eufol, and citrostadienol) [9][10][11][12][13][14] indicated that these were absent, suggesting other active principles that could act as antiproliferative agents, like compound 1. (Table 2).…”

Section: Resultsmentioning

confidence: 99%

Antiproliferative Effect of Synadenium grantii Hook f. stems (Euphorbiaceae) and a Rare Phorbol Diterpene Ester

et al. 2016

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Synadenium grantii is frequently used for the treatment of various diseases such as allergies, gastric disorders, and especially cancer. The aim of this study was to evaluate the possible antiproliferative potential of the methanol extract, fractions, and pure compounds from the stems of S grantii Phytochemical analysis was carried out by conventional chromatographic techniques, and the antiproliferative activity was analyzed using the sulforhodamine B assay and an MTT-based assay. Nonpolar fraction and its subfractions from the stems of S grantii exhibited promising cytostatic effect against several human tumor cell lines (glioma, breast, kidney, and lung), with total grown inhibition values ranging from 0.37 to 2.9 μg/mL. One of the active principles of this plant was identified as a rare phorbol diterpene ester, denoted as 3,4,12,13-tetraacetylphorbol-20-phenylacetate. This compound demonstrated antiproliferative activity against glioma, kidney, lung, and triple-negative breast cancer cell lines. These results demonstrate that S grantii stems produce active principles with relevant antiproliferative potential.

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“…The GC/MS analysis and comparison with NIST and Wiley databases allowed the identification of some compounds present in the fractions; among them, euphol, the major compound already described in the Hex, and other triterpenes like lanosterol, cycloartenol, tirucallol, taraxasterol and lupeol, besides the sitosterol, a steroid already identified in this specie. [11,14] In the Dcm and Eth, diterpenes with a tigliane core were suggested, as phorbol 12,13,20triacetate, 4β-phorbol, and 3-desoxo-3,16-dihydroxy-12-desoxyphorbol 3,13,16,20-tetraacetate. Diterpenes are described as taxonomic markers for the Euphorbiaceae family and the cytotoxic activity of species of the genus Euphorbia have been attributed to them.…”

Section: Chemical Analysismentioning

confidence: 99%

“…The cytotoxic potential of the latex from E. umbellata has been described previously for B16F10 (melanoma), HRT-18 (colon cancer) and HeLa (cervical adenocarcinoma) cell lineages; [11,13] latex extracts have been evaluated against HRT-18, HeLa and Jurkat (leukemia) lineages, with the hexane extract presenting higher cytotoxicity than the chloroform, ethyl acetate and methanol extracts. [14] Chemical studies suggested the presence of several compounds that could be related with the cytotoxic activity of E. umbellata; therefore, a preliminary cytotoxic evaluation was developed with Hex and its fractions.…”

Section: Cytotoxic Assaymentioning

confidence: 99%

Enriched Terpenes Fractions of the Latex of Euphorbia umbellata Promote Apoptosis in Leukemic Cells

Cruz

Kanunfre

Andrade

et al. 2020

Chemistry & Biodiversity

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The current study was carried out by a bioguided fractionation of a hexane extract of the latex of Euphorbia umbellata against leukemic cells. Samples were analyzed by NMR, GC/MS, triterpenes quantification, and MTT reduction assay. Morphological, cell cycle, mitochondrial membrane potential and caspases 3/7 analyses were performed for the dichloromethane and ethanol fractions, and selectivity index for the dichloromethane fraction. NMR analysis presented characteristic signals of terpenes and steroids, data were confirmed by the quantification of triterpenes and GC/MS analysis. MTT reduction assay demonstrated that HL‐60 was the most sensitive cell lineage against dichloromethane and ethanol fractions. Compounds of these matrices caused morphological changes compatible with apoptosis induction, altered cell cycle, increment of depolarized population cells and activation of caspases 3/7. Selectivity indices were higher than 22.44. Bioguided‐fractionation study showed that samples of the latex of E. umbellata raised the activity of the phytocomplex against leukemic cells, and the cytotoxicity can be associated with an apoptosis pathway.

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Antitumoural effect of Synadenium grantii Hook f. (Euphorbiaceae) latex

Cited by 31 publications

References 35 publications

Eutirucallin: A Lectin with Antitumor and Antimicrobial Properties

Eutirucallin: A Lectin with Antitumor and Antimicrobial Properties

Antiproliferative Effect of Synadenium grantii Hook f. stems (Euphorbiaceae) and a Rare Phorbol Diterpene Ester

Enriched Terpenes Fractions of the Latex of Euphorbia umbellata Promote Apoptosis in Leukemic Cells

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