Antiulcer Effects of Ethyl acetate Fraction of Carpolobia lutea Leaf

Abstract

Carpolobia lutea leaves (CLL) (Polygalaceae) were earlier screened and the antiulcer ethnomedicinal claim established. This article seeks to quantitatively isolate, elucidate the active compounds from most active CLL fraction. Fractionation was by semi-preparative HPLC; the active fraction was subjected to radical scavenging assays (RSA) and quantification of the total phenolic content (TPC) were also executed. Results: Ethyl acetate fraction (EAF) was observed to be the most pharmacologically active antiulcer fraction when screened using acute ulcer models induced in rodents. The EAF demonstrated significant (p < 0.05-0.001) antiulcer activity in various in vivo induced ulceration models by reducing the ulcer index and increasing the preventive ratio. The EAF demonstrated > 70% in TPC and < 20 % in RSA. Cinnamic and coumaric acids derivatives were isolated from EAF. Cinnamic acids have been implicated and patented as antiulcer agent. Isolated compounds could in part mediate the observed pharmacological activities which lend credence to its ethnobotanical uses.

The aphrodisiac herb Carpolobia: A biopharmacological and phytochemical review

“…In sum, the pharmacological studies of Carpolobia reveal antitrypanosomal and antileishmanial properties[ 37 ] antiplasmodial and antimalarial properties,[ 38 39 ] contraceptive, estrogenic, and antiestrogenic properties,[ 13 32 ] antiulcerogenic and antidiarrheal properties,[ 30 36 37 ] gastroprotective effect,[ 33 ] antinociceptive properties,[ 17 ] anti-inflammatory and antipyretic effects,[ 41 ] antidiabetic and hypolipidemic effects,[ 44 ] antimicrobial properties,[ 31 35 ] antioxidants and amino acid profile,[ 42 ] and analgesic activity,[ 44 ] which reflects reviews of saponin biological activities. But no aphrodisiac activities,[ 6 8 13 14 ] antihemorrhoidal properties,[ 63 ] or neuropharmacological effects[ 43 ] were seen among the reviewed effects of the saponins.…”

“…The leaf ethanol extract of CL shows antidiarrheal and antiulcerogenic potential: In particular, dose-dependent gastroprotective and antidiarrheal effects in rodents. [ 30 ] The gastroprotective effects of the leaf were more pronounced from the ethyl acetate extract than the n -hexane, chloroform, and ethanol leaf fractions of CL,[ 33 ] and the ethyl acetate fraction showed dose-dependent effects in all models of antiulcer activity as investigated in rats with the leaf extract[ 36 ] and the stem extract. [ 37 ]…”

In vitro Anti-cholinesterase and Anti-oxidant Activity of Three Standardised Polyherbal Products Used for Memory Enhancing in Ethnomedicine of South-East Nigeria

“…A 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was employed, to determine by a spectroscopic method, the anti-oxidant capability via free radical scavenging activity of polyherbal extracts using a method described previously ( 33 ). Stock solutions of each of the polyherbals (5 mg/mL) were diluted to final concentrations of 200 μg/mL, 100 μg/mL, 50 μg/mL, 25 μg/ mL, 12.5 μg/mL, and 6.25 μg/mL in ethanol.…”

Anti-Inflammatory and Anti-Nociceptive Activities of Stem-Bark Extracts and Fractions of Carpolobia Lutea (Polygalaceae)

“…Reports on the biological screening of the leaf extracts and fractions of CL have been reviewed[18] as gastroprotective,[19] antinociceptive,[20] antidiarrheal,[21] antimicrobial,[22] anti-inflammatory,[23] neuropharmacological,[24] and antiulcer effects;[25] isolation of cinnamoyl 1-deoxyglucosides and cinnamic acid derivatives[26] from the leaves of CL has also been reported. No pharmacological investigations on the anti-inflammatory properties of the stems and stem-bark extract exist to our knowledge in any literature to repudiate or acknowledge ethnomedicinal claim.…”