Antiulcer Effects of the Tripeptide PGP and Its Possible Metabolites (PG, GP, Glycine, and Proline) in Different Models of Ulcer Induction in Rats

Zhuikova, S. E.; Badmaeva, K E; Бакаева, З. В.; Samonina, G. E.

doi:10.1023/b:bibu.0000043776.19853.65

Cited by 5 publications

(4 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Where, Pt = paw thickness at time t, Po = initial paw thickness, PC = Increase in thickness of paw of the control group and PT = Increase in thickness of paw of the treatment group [ 27 ].…”

Section: Methodsmentioning

confidence: 99%

Evaluation of anti-inflammatory and gastric anti-ulcer activity of Phyllanthus niruri L. (Euphorbiaceae) leaves in experimental rats

Mostofa

Ahmed

Begum

et al. 2017

BMC Complement Altern Med

View full text Add to dashboard Cite

BackgroundThe medicinal plants signify a massive basin of potential phytoconstituents that could be valuable as a substitute to allopathic drugs or considered as an analogue in drug development. Phyllanthus niruri L. (Euphorbiaceae) is generally used in traditional medicine to treat ulcer and inflammation. In this project we investigated the methanolic extract of leaves of Phyllanthus niruri for anti-inflammatory and anti-ulcer activity.MethodsThe anti-inflammatory activity of methanol extract of Phyllanthus niruri leaves was evaluated at the doses of 100, 200 and 400 mg/kg, p.o. while using ibuprofen (20 mg/kg, p.o) as the standard drug. The animals used were Swiss albino rats. Inflammation was induced by injecting 0.1 ml carrageenan (1% w/v) into the left hind paw. Paw tissues from the different groups were examined for inflammatory cell infiltration. On the other hand, antiulcer activity of methanolic extract of P. niruri leaves at the doses of 100, 200 and 400 mg/kg, p.o. were examined against ethanol-acid induced gastric mucosal injury in the Swiss albino rats - keeping omeprazole (20 mg/kg, p.o.) as reference. The rats were dissected and the stomachs were macroscopically examined to identify hemorrhagic lesions in the glandular mucosa.Results P. niruri significantly (p < 0.01) decreased carrageenan-induced paw edema; it exhibited a reduction of 46.80%, 55.32% and 69.14% at doses of 100, 200 and 400 mg/kg, respectively. These findings were further supported by the histological study. The methanolic extract also disclosed good protective effect against ethanol-acid induced gastric mucosal injury in the rats. Administration of the extract’s doses (100, 200 and 400 mg/kg) demonstrated a significant (p < 0.01) reduction in the ethanol- acid induced gastric erosion in all the experimental groups when compared to the control. The methanolic extract at the higher dose (400 mg/kg) resulted in better inhibition of ethanol-acid induced gastric ulcer as compare to omeprazole (20 mg/kg). Histological studies of the gastric wall revealed that toxic control rats revealed mucosal degeneration, ulceration and migration of numerous inflammatory cells throughout the section. On the other hand, MEPN treatment groups showed significant regeneration of mucosal layer and significantly prevented the formation of hemorrhage and edema.ConclusionsThe investigation suggests that methanolic extract of P. niruri leaf possess anti-inflammatory activity and promotes ulcer protection as ascertained by regeneration of mucosal layer and substantial prevention of the formation of hemorrhage and edema.

show abstract

Section: Methodsmentioning

confidence: 99%

Evaluation of anti-inflammatory and gastric anti-ulcer activity of Phyllanthus niruri L. (Euphorbiaceae) leaves in experimental rats

Mostofa

Ahmed

Begum

et al. 2017

BMC Complement Altern Med

View full text Add to dashboard Cite

show abstract

“…The stomach of each animal was excised and opened along the greater curvature. Lesion score was recorded as described below [15]. The gastromucosal protective effects of the extracts were compared to those of ranitidine (100 mg/kg).…”

Section: Ethanol-induced Gastric Mucosal Lesions In Albino Ratsmentioning

confidence: 99%

“…All samples of the fractions were given 30 min prior to ethanol at a dose of 20 mg/kg. Lesion score was recorded as described above [15].…”

Section: Ethanol-induced Gastric Mucosal Lesions In Albino Ratsmentioning

confidence: 99%

Anti-inflammatory and gastromucosal protective effects of Calotropis procera (Asclepiadaceae) stem bark

Tour

Talele²

2011

J Nat Med

View full text Add to dashboard Cite

This study was aimed at evaluating the anti-inflammatory and gastromucosal protective effect of chloroform extract (CH) and hydroalcoholic extract (HE) of the stem bark of Calotropis procera obtained successively by cold maceration. The anti-inflammatory effect of the CH and HE extracts of the stem bark of Calotropis procera against carrageenan-induced paw oedema and also its gastromucosal protective effect has been studied by using two acute models: aspirin (100 mg/kg, p.o.) and ethanol (96%) in albino rats. CH and HE extracts showed significant anti-inflammatory activity at 200 and 400 mg/kg, while CH extract at 400 mg/kg was also found to have a significant gastromucosal protective effect. As part of investigations to obtain compounds with anti-inflammatory and gastromucosal protective effects in this work, a bioassay was carried out with fractions obtained from the CH extract with n-hexane (NF1), 1-butanol (BF1), ethyl acetate (EF1) and chloroform (CF1). The HE extract of the stem bark was fractionated with n-hexane (NF2), 1-butanol (BF2), ethyl acetate (EF2), chloroform (CF2) and water (WF2). The fractions were evaluated for their anti-inflammatory and gastromucosal protective effects. Fractions NF1, CF1, BF2 and EF2 (20 mg/kg) showed significant anti-inflammatory activity, while NF1 and BF2 (20 mg/kg) also showed gastromucosal protective effects. The results obtained for gastromucosal protective effects were also well supported by histopathological examination of the open excised rat stomach.

show abstract

“…The stomach of each animal was excised and opened along the greater curvature. Ulcer score was recorded as mentioned in Histopathological studies were performed to confirm the ulcer score (Zhuikova et al 2004).…”

Section: Gastric Lesions Induced By Ethanolmentioning

confidence: 99%

Gastric antiulcer and antiinflammatory activities of Calotropis procera stem bark

Tour¹,

Talele

2011

Rev. bras. farmacogn.

View full text Add to dashboard Cite

Abstract:In recent years, a widespread search has been launched to identify new antiinflammatory and antiulcer-drugs from natural sources. The study was aimed at evaluating the antiinflammatory and antiulcer activity of chloroform extract (CH) and hydroalcoholic extract (HE) of the stem bark of Calotropis procera (Aiton) W.T. Aiton, Apocynaceae, obtained successively by cold maceration. The antiinflammatory effect of the CH and HE extracts of the stem bark of the C. procera against carrageenan-induced paw oedema and also its antiulcer activity by using two acute models: Aspirin (100 mg/kg, p.o.) and ethanol (96%, 1 mL/200 g) in albino rats have been studied and found to be significant at 200 and 400 mg/kg when compared to the standard drugs. As a part of investigations to obtain compounds with antiinflammatory and antiulcer activity in this work, a bioassay was carried out with fractions obtained from chloroform extract with n-hexane (NF1), 1-butanol (BF1), ethyl acetate (EF1) and chloroform (CF1). The hydroalcoholic extract (HE) of the stem bark was fractionated with n-hexane (NF2), 1-butanol (BF2), ethyl acetate (EF2), chloroform (CF2) and water (WF2). The fractions were freezedried and evaluated for its antiinflammatory and antiulcer activity. Fractions NF1, CF1, BF2 and EF2 (20 mg/kg) showed significant antiinflammatory and antiulcer activity. The results obtained for antiulcer activity were also supported well by the histopathological examination of the open excised rat stomach. Further experiments are underway to determine which phytoconstituents are involved in antiinflammatory and antiulcer activities as well as mechanisms involved in gastroprotection.

show abstract

Antiulcer Effects of the Tripeptide PGP and Its Possible Metabolites (PG, GP, Glycine, and Proline) in Different Models of Ulcer Induction in Rats

Cited by 5 publications

References 6 publications

Evaluation of anti-inflammatory and gastric anti-ulcer activity of Phyllanthus niruri L. (Euphorbiaceae) leaves in experimental rats

Evaluation of anti-inflammatory and gastric anti-ulcer activity of Phyllanthus niruri L. (Euphorbiaceae) leaves in experimental rats

Anti-inflammatory and gastromucosal protective effects of Calotropis procera (Asclepiadaceae) stem bark

Gastric antiulcer and antiinflammatory activities of Calotropis procera stem bark

Contact Info

Product

Resources

About