Antiurolithiatic Activity of Aqueous Extract of Ziziphus lotus on Ethylene Glycol-Induced Lithiasis in Rats

Abstract

In Morocco, Ziziphus lotus is commonly used as an urolithiatic agent in the traditional medicine. To confirm this effect, an aqueous extract of Ziziphus lotus (AEZL) has been studied in ethylene glycolinduced urolithiasis model of rats. 30 male rats were randomly divided into five groups of six animals each. Group I served as a vehicle control and received distilled water (0.5 ml/100 g p.o.). All remaining groups received calculi inducing treatment for 28 days, comprised of 0.75% v/v ethylene glycol with 1% w/v ammonium chloride in drinking water ad libitum for 3 days followed by only 0.75% v/v ethylene glycol for 25 days. Group II served as lithiatic control and received distilled water (0.5 ml/100 g p.o.). Group III served as curative treatment group and received AEZL at doses of 150 mg/kg from 14th day to 28th day. Group VI served as preventive treatment group and received AEZL at doses of 150 mg/kg from 1st day to 28th day. Group V served as therapeutic and received a drug "Cystone" at dose of 750 mg/kg from 14 th day to 28 th day. The extract treatment decreased the levels of oxalate and calcium in urine. Crystalluria analysis showed that untreated rats excreted large CaOx monohydrate and few dihydrate crystals while treated animals excreted mostly small CaOx dihydrate crystals. Significant similarity was observed between preventive and therapeutic anti-urolithiatic effect of AEZL and anti-urolithiatic effect of cystone (P<0.001). These results demonstrated that AEZL have an anti-urolithiatic effect with preventive and therapeutic treatments in this experimental condition.

A case report of a giant bladder stone (12 × 8 cm, 610 g)

“…Several methods are used by the local population including Extracorporeal shock wave lithotripsy [ [25] , [26] ], surgery and traditional therapy based in the use of medicinal plants [ [18] , [19] ]. The transurethral cystolithotripsy is the treatment of choice for removing bladder stones [10] .…”

The Anti-urolithiatic effect of the roots of Saussurea costus (falc) Lipsch agonist ethylene glycol and magnesium oxide induced urolithiasis in rats

“…24-hour urine samples were collected from rats housed in individual metabolic cages at the end of the calcium oxalate and struvite assays. Urine was collected in a 50 ml tube for microscopic study of crystal deposition and cytobacteriological examination of the urine, and 0.5 ml of the urine sample was placed in a Malassez slide, from which the type and number of crystals were identified by polarized light microscopy ( Chakit et al, 2022 ). The remaining urine was placed in Eppendorf tubes, to which a drop of hydrochloric acid was added and kept at 4 °C ( Rashid et al, 2023 ).…”

Evaluation of Acute Toxicity, Antidepressant, Antioxidant Activities and Memory Ability of Curcuma longa Methanolic Extract in High fructose-treated rat

“…The rats used in this manipulation were divided into several groups and had free access to tap water for drinking and a standard diet (provided by the Alf Sahel animal feed marketing company). The animals' food was composed of: 13% crude protein, 2% fat, 0.3% phosphorus, 9% mineral matter, 15% cellulose, 1% calcium, 500 IU of vitamin A, 75 IU Vitamin D, 1 IU of Vitamin E. Other animals were subjected to a standard diet and a high-calorie diet rich in fructose 23% dissolved in water (30ml/Rat) for 2 months [8]. The consumption of the fructose solution for each group of animals was measured daily during the 2 months of the diet.…”

“…It is a behavioral disorder like depression and anxiety.. Much of the therapeutic effect of medicinal plants has been attributed to plant secondary metabolites such as polyphenols. In particular, natural phenols play an important role in protection against a number of pathological disorders, such as urolithiasis, atherosclerosis, brain dysfunction and obesity [2,[8][9][10].…”