Antiviral activity of a polysaccharide from Radix Isatidis (Isatis indigotica Fortune) against hepatitis B virus (HBV) in vitro via activation of JAK/STAT signal pathway

Wang, Tianbao; Wang, Xinwei; Zhuo, Ya; Si, Changyun; Yang, Lele; Meng, Lijun; Zhu, Bin

doi:10.1016/j.jep.2020.112782

Cited by 53 publications

(29 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, the HBV inhibition activities of polysaccharide from Radix isatidis were mediated by the stimulation of the IFNα-dependent JAK/STAT signal pathway and the initiation of protein expression against HBV (Wang et al, 2020). Swertisin obtained from…”

Section: Hepatitis B Virusmentioning

confidence: 99%

Medicinal plants: Treasure for antiviral drug discovery

Ali

Sheikh

Rather

et al. 2021

Phytotherapy Research

View full text Add to dashboard Cite

The pandemic of viral diseases like novel coronavirus (2019‐nCoV) prompted the scientific world to examine antiviral bioactive compounds rather than nucleic acid analogous, protease inhibitors, or other toxic synthetic molecules. The emerging viral infections significantly associated with 2019‐nCoV have challenged humanity's survival. Further, there is a constant emergence of new resistant viral strains that demand novel antiviral agents with fewer side effects and cell toxicity. Despite significant progress made in immunization and regenerative medicine, numerous viruses still lack prophylactic vaccines and specific antiviral treatments that are so often influenced by the generation of viral escape mutants. Of importance, medicinal herbs offer a wide variety of therapeutic antiviral chemotypes that can inhibit viral replication by preventing viral adsorption, adhering to cell receptors, inhibiting virus penetration in the host cell, and competing for pathways of activation of intracellular signals. The present review will comprehensively summarize the promising antiviral activities of medicinal plants and their bioactive molecules. Furthermore, it will elucidate their mechanism of action and possible implications in the treatment/prevention of viral diseases even when their mechanism of action is not fully understood, which could serve as the base for the future development of novel or complementary antiviral treatments.

show abstract

Section: Hepatitis B Virusmentioning

confidence: 99%

Medicinal plants: Treasure for antiviral drug discovery

Ali

Sheikh

Rather

et al. 2021

Phytotherapy Research

View full text Add to dashboard Cite

show abstract

“…Radix isatidis is traditionally used to treat viral and bacterial infections in China. Radix Isatidis polysaccharide (RIP) is an effective treatment for several viruses, including HSV-2 [ 68 ], influenza A virus [ 69 ], and HBV [ 70 ]. According to Wang, its antiviral activity may be due to the enhancement of the immune response.…”

Section: Fighting Viruses By Modulating Immunitymentioning

confidence: 99%

“…In HepG2.2.15 cells infected with HBV, RIP treatment decreased the levels of both HBsAg and HBeAg. Results from ELISA showed that RIP upregulates IFN-α in cells and activates the JAK/STAT signaling pathway by promoting the phosphorylation of related kinases, which leads to a protective effect against HBV [ 70 ].…”

Section: Fighting Viruses By Modulating Immunitymentioning

confidence: 99%

Development of Broad-Spectrum Antiviral Agents—Inspiration from Immunomodulatory Natural Products

Zhang

Xiong

Song

et al. 2021

Viruses

View full text Add to dashboard Cite

Developing broad-spectrum antiviral drugs remains an important issue as viral infections continue to threaten public health. Host-directed therapy is a method that focuses on potential targets in host cells or the body, instead of viral proteins. Its antiviral effects are achieved by disturbing the life cycles of pathogens or modulating immunity. In this review, we focus on the development of broad-spectrum antiviral drugs that enhance the immune response. Some natural products present antiviral effects mediated by enhancing immunity, and their structures and mechanisms are summarized here. Natural products with immunomodulatory effects are also discussed, although their antiviral effects remain unknown. Given the power of immunity and the feasibility of host-directed therapy, we argue that both of these categories of natural products provide clues that may be beneficial for the discovery of broad-spectrum antiviral drugs.

show abstract

“…7 Moreover, polysaccharides from R Isatidis were identified as the potential active ingredients. 18 The anti-viral activity of F Isatidis was also investigated, with relatively fewer reports. Deng et al highlighted the n-butanol fraction of the F Isatidis extract as the principal anti-viral effective substantial foundation.…”

Section: Introductionmentioning

confidence: 99%

“…As mentioned above, chemical characterization of Ii 17,20,21 has been reported, as well as preclinical studies of single ingredients [22][23][24][25][26] or aqueous extracts. 11,14,18,19 However, there is still a need to elucidate the pharmacodynamic material basis of Ii in a more comprehensive manner. Here, we applied a network analysis-based integrated strategy to explore the pharmacodynamic material basis and potential mechanisms of the anti-viral and antiinflammatory effect of Ii.…”

Section: Introductionmentioning

confidence: 99%

A Network Pharmacology-Based Investigation to the Pharmacodynamic Material Basis and Mechanisms of the Anti-Inflammatory and Anti-Viral Effect of Isatis indigotica

Deng

et al. 2021

DDDT

View full text Add to dashboard Cite

Isatis indigotica (Ii) is a cruciferous herb that is widely distributed in China, and its roots and leaves have been used in two renowned antipyretic detoxicate crude drugs in Chinese Pharmacopoeia, Radix (R) and Folium (F) Isatidis. However, the pharmacodynamic material basis and underlying mechanisms of the herbal efficacy remained to be elucidated. Methods: Ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was adopted for the chemical profiling of R and F Isatidis. The active ingredients were screened out through the prediction of gastrointestinal absorption and druglikeness analysis using SwissADME. A herb-ingredient-target network was constructed through target prediction of the herbal active ingredients and anti-inflammation or anti-viral properties, followed by protein-protein interaction analysis. Then, the potential relevant signaling pathways were predicted by pathway enrichment. Finally, for verification, RAW 264.7 cell line was adopted to examine the anti-inflammatory and anti-viral activities of 6 representative ingredients in Ii. Results: Seventy-three compounds have been identified from Ii through UPLC-Q-TOF-MS. A total of 17 potential active ingredients were screened through pharmacokinetics and druglikeness evaluation using SwissADME. It was shown that key targets might include TNF, AKT1, SRC, IL2, CASP9, and CASP3 in our herb-ingredient-target network, and isovitexin, a flavonoid, tended to participate in the inflammatory response, indoles were more likely to affect the cell proliferation processes, and lignans might have a broader affinity to key targets than the other active ingredients, such as regulating immune system (targeting IL-2) and PI3K-Akt signaling pathway. In vitro, indigo and secoisolariciresinol diglucoside markedly reduced TNF-α expression in Poly (I: C)-incubated cells. Isovitexin significantly inhibited TNF-α expression, and isatin treatment markedly reduced IL-1β expression in LPS-incubated cells. Conclusion:As the pharmacodynamics material basis of Ii, indoles, lignans, and flavonoids are believed to confer beneficial properties through various cellular aspects with multiple signaling pathways involved.

show abstract

Antiviral activity of a polysaccharide from Radix Isatidis (Isatis indigotica Fortune) against hepatitis B virus (HBV) in vitro via activation of JAK/STAT signal pathway

Cited by 53 publications

References 44 publications

Medicinal plants: Treasure for antiviral drug discovery

Medicinal plants: Treasure for antiviral drug discovery

Development of Broad-Spectrum Antiviral Agents—Inspiration from Immunomodulatory Natural Products

A Network Pharmacology-Based Investigation to the Pharmacodynamic Material Basis and Mechanisms of the Anti-Inflammatory and Anti-Viral Effect of Isatis indigotica

Contact Info

Product

Resources

About