Antiviral activity of canine interferon lambda 3 expressed using a recombinant adenovirus against canine coronavirus, canine parvovirus, and canine distemper virus

Kim, Dong‐Hwi; Han, Sang-Hoon; Go, Hyeon‐Jeong; Kim, Da-Yoon; Kim, Jae-Hyeong; Lee, Joong‐Bok; Park, Seung‐Yong; Song, Chang‐Seon; Lee, Sang‐Won; Choi, In‐Soo

doi:10.1007/s11259-022-10000-1

Cited by 7 publications

(4 citation statements)

References 27 publications

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“…So far, it has been proved that the anti-inflammatory drug indomethacin has effective antiviral features to fight against CCoV infection, through action both on viral replication and block of viral RNA synthesis [ [46] , [47] , [48] ]. Recent advances show as a recombinant adenovirus, which expresses canine interferon lambda 3, has an antiviral activity against CCoV [ 49 ]. Due to high toxicity of the conventional antiviral drugs, numerous natural substances are being recently tested to conceive new therapeutics against viral infections.…”

Section: Discussionmentioning

confidence: 99%

Fungal metabolite 6-pentyl-α-pyrone reduces canine coronavirus infection

Cerracchio,

Del Sorbo,

Serra

et al. 2024

Heliyon

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Section: Discussionmentioning

confidence: 99%

Fungal metabolite 6-pentyl-α-pyrone reduces canine coronavirus infection

Cerracchio,

Del Sorbo,

Serra

et al. 2024

Heliyon

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“…Current 2022 investigations imply that the IFN-λR1 is also expressed within gingival keratinocytes, with in vitro IFN-λ1 stimulation at low doses stimulating RIG-I/TLR3, with both PRRs recognising viral RNA without evoking high expression of pro-inflammatory cytokines, like IL-6, and therefore may be of consideration as an anti-viral [210]. Whereas IFN-λ3 expression in a vector is being looked at to counter a variety of dog-affecting pathogens like canine coronavirus (CCoV), parvovirus (CPV), as well as distemper virus (CDV) [211]. During 2020, in vivo expression of a recombinant type III IFN (IFN-λ2, IFN-λ3), during Rabies virus infection (RABV) was shown to have an anti-viral response administered intranasally and reducing viral load in a neurotropic virus.…”

Section: Early Type III Interferon Research During Viral Diseasesmentioning

confidence: 99%

Dr Jekyll and Mr Hyde: From Two Branches of Immune Response to Three Types of Interferon Response

Brown,

Imarogbe,

Fricke

et al. 2023

Preprint

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Interferons were the original prototype cytokine system discovered in 20th-century research. As the name implies, they were originally thought to be synthesised and secreted between cells. Thanks to technological advances, the processes involved in protein secretion can be explained comparatively more clearly at both the genetic and biochemical levels. The discovery of interferon (IFN) occurred when genetic research was still in its infancy. Franklin and Wilkins discovered the structure and function of deoxyribonucleic acid (DNA) at the same time as Crick and Watson; however, Isaacs and Lindemann, two scientists, described the first IFN in 1957. Mutations can be caused by inherent genetic protein synthesis and during infection as well as within IFN regulation pathways affecting cell proliferation. This remains central to host cell IFN synthesis and effects through IFN protein receptor subunits defined by 6 protein domains. Type II IFN is key to immune cell function secreted by a variety of immune cells, mainly natural killer (NK) as well as T cells. Single–stranded and/or double–stranded RNA/DNA viruses, as well as bacterial infections (e.g., Escherichia coli) and fungal infections (e.g., Aspergillus), also affect IFN regulation. Pathogenic proteins utilise intra/extracellular proteins that sense foreign antigens like Toll–like Receptors (TLRs), affected by mutations within the human cellular IFN transduction pathways. Since the discovery of the third IFN type in 2003, when immune cell phenotypes were further characterised, questions remain about the immunological mechanisms contributing to the regulation of the innate and adaptive host immune system. Alterations in the synthesis of type I/II/III host IFNs can differentially and beneficially alter homeostatic cellular pathways in pathological disease, with type I IFN being synthesised in cancer as well as by homeostatic cells. Therefore, considered here are the overall IFN molecular, cell regulatory mechanisms in the context of immune cell research developments.

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“…11,13 Although the IFNλ receptor is mainly expressed on epithelial surfaces, its expression would be effective in suppressing the proliferation of respiratory viruses such as influenza virus and RSV, which are confined to the lung epithelium. 14 In this study, a recombinant adenovirus vector with a tetracycline operator, developed in our previous study, 15,16 was used to express human interferon lambda-4 (huIFNλ4). To assess the safety of the recombinant adenovirus containing huIFNλ4 (rAd-huIFNλ4), toxicity tests were conducted in compliance with Good Laboratory Practice (GLP) standards.…”

Section: Introductionmentioning

confidence: 99%

“…In this study, a recombinant adenovirus vector with a tetracycline operator, developed in our previous study, 15,16 was used to express human interferon lambda‐4 (huIFNλ4). To assess the safety of the recombinant adenovirus containing huIFNλ4 (rAd‐huIFNλ4), toxicity tests were conducted in compliance with Good Laboratory Practice (GLP) standards.…”

Section: Introductionmentioning

confidence: 99%

Antiviral activity of adenoviral vector expressing human interferon lambda‐4 against influenza virus

Kim,

Lim

et al. 2024

Journal of Medical Virology

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Interferon lambda (IFNλ), classified as a type III IFN, is a representative cytokine that plays an important role in innate immunity along with type I IFN. IFNλ can elicit antiviral states by inducing peculiar sets of IFN‐stimulated genes (ISGs). In this study, an adenoviral vector expression system with a tetracycline operator system was used to express human IFNλ4 in cells and mice. The formation of recombinant adenovirus (rAd‐huIFNλ4) was confirmed using immunohistochemistry assays and transmission electron microscopy. Its purity was verified by quantifying host cell DNA and host cell proteins, as well as by confirming the absence of the replication‐competent adenovirus. The transduction of rAd‐huIFNλ4 induced ISGs and inhibited four subtypes of the influenza virus in both mouse‐derived (LA‐4) and human‐derived cells (A549). The antiviral state was confirmed in BALB/c mice following intranasal inoculation with 109 PFU of rAd‐huIFNλ4, which led to the inhibition of four subtypes of the influenza virus in mouse lungs, with reduced inflammatory lesions. These results imply that human IFNλ4 could induce antiviral status by modulating ISG expression in mice.

show abstract

Antiviral activity of canine interferon lambda 3 expressed using a recombinant adenovirus against canine coronavirus, canine parvovirus, and canine distemper virus

Cited by 7 publications

References 27 publications

Fungal metabolite 6-pentyl-α-pyrone reduces canine coronavirus infection

Fungal metabolite 6-pentyl-α-pyrone reduces canine coronavirus infection

Dr Jekyll and Mr Hyde: From Two Branches of Immune Response to Three Types of Interferon Response

Antiviral activity of adenoviral vector expressing human interferon lambda‐4 against influenza virus

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