Antiviral Activity of Chicken Cathelicidin B1 Against Influenza A Virus

Peng, Lianci; Du, Wenjuan; Balhuizen, Melanie D.; Haagsman, Henk P.; Haan, Cornelis A. M. de; Veldhuizen, Edwin J.A.

doi:10.3389/fmicb.2020.00426

Cited by 23 publications

(16 citation statements)

References 36 publications

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“…In this study, we showed that APEC upregulates gene expression of CATH-B1 in macrophages but not of CATH-1, -2, -3. Recently, it was found by our group that CATH-B1 has strong antiviral activity against influenza A viruses in vitro [ 14 ]. In this study, the immunomodulatory effect of CATH-B1 on APEC-, LPS- or ODN-2006-activated chicken macrophages was explored.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, in ovo administration of the all D-amino acid enantiomer of CATH-2 (D-CATH-2) at embryonic day 18 resulted in a protective effect against avian pathogenic E. coli (APEC) infection up to 7 days after hatch [ 13 ]. However, less is known about the activities of CATH-B1, which means that it is challenging to properly compare functionalities and activities [ 14 ]. When comparing expression patterns, one clear difference between CATH-1, -2, -3 and CATH-B1 is apparent: using immunostaining and mass spectrometry CATH-1, -2, -3 were detected in heterophils [ 15 ], while CATH-B1 was shown to be produced by epithelial cells in the bursa of Fabricius, although an extended description of CATH-B1 protein expression among cells/tissues was not described [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

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The immunomodulatory effect of cathelicidin-B1 on chicken macrophages

Peng

Scheenstra

Harten

et al. 2020

Vet Res

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Cathelicidins (CATHs) play an important role in the innate immune response against microbial infections. Among the four chicken cathelicidins, CATH-B1 is studied the least. In this study, the effect of CATH-B1 on the macrophage response towards avian pathogenic E. coli (APEC) and bacterial ligands was investigated. Our results show that APEC induced CATH-B1 gene expression in both a chicken macrophage cell line (HD11 cells) and primary macrophages, while expression of the other three CATHs was virtually unaffected. While the antimicrobial activity of CATH-B1 is very low under cell culture conditions, it enhanced bacterial phagocytosis by macrophages. Interestingly, CATH-B1 downregulated APEC-induced gene expression of pro-inflammatory cytokines (IFN-β, IL-1β, IL-6 and IL-8) in primary macrophages. In addition, CATH-B1 pre-incubated macrophages showed a significantly higher gene expression of IL-10 after APEC challenge, indicating an overall anti-inflammatory profile for CATH-B1. Using isothermal titration calorimetry (ITC), CATH-B1 was shown to bind LPS. This suggests that CATH-B1 reduces toll like receptor (TLR) 4 dependent activation by APEC which may partly explain the decreased production of pro-inflammatory cytokines by macrophages. On the contrary, direct binding of CATH-B1 to ODN-2006 enhanced the TLR21 dependent activation of macrophages as measured by nitric oxide production. In conclusion, our results show for the first time that CATH-B1 has several immunomodulatory activities and thereby could be an important factor in the chicken immune response.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The immunomodulatory effect of cathelicidin-B1 on chicken macrophages

Peng

Scheenstra

Harten

et al. 2020

Vet Res

Self Cite

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“…Another study pertaining to this subject of beetle defensin revealed the anticancer effect of a combination of this gene with synthetic peptides on certain cancers (56). Peng et al (17)(18) concluded that chicken cathelicidin-2 and -B1 have both antibacterial and immunomodulatory roles when they incubated with chicken macrophages cell line infected by pathogenic E. coli through neutralisation of certain bacterium and decreases in ammation response.…”

Section: Discussionmentioning

confidence: 99%

“…Considering other peptides, gene duplication leads to the formation of mature peptides CATH-1 and CATH-3 having about 70% similar sequence (5)(6)(7).However, only their antibacterial properties have been extensively studied in majority of research (8)(9)(10)(11)(12). Several studies have been investigated the antimicrobial and immunomodulatory potentialities of chicken cathelicidin-2 through activating toll like receptor-2 and neutralization of LPS (13)(14)(15)(16)(17)(18).…”

Section: Introductionmentioning

confidence: 99%

Anticancer activity of chicken cathelicidin peptides against different types of cancer

Mahmoud

Al-Hejin

Abujamel

et al. 2021

Preprint

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This study served as the pioneer in studying the anti-cancer role of chicken cathelicidin peptides. Chicken cathelicidins were used as anticancer agent against the breast cancer cell line (MCF-7) and human colon cancer cell line (HCT116). An in vivo investigation was also achieved to evaluate the role of chicken cathelicidin in Ehrlich ascites cell (EAC) suppression as a tumor model after subcutaneous implantation in mice. In addition, the mechanism of action of the interaction of cationic peptides with breast cancer cell line MCF-7 was also investigated. It was found during the study that exposure of cell lines to higher concentration of chicken cathelicidin for 72 h reduced cell lines growth rate by 90%-95%. These peptides demonstrated down-regulation of (cyclin A1 and cyclin D genes) which are essential for G1/S phase transient and S/G2 phase and consequently causes “prometaphase arrest” ultimately leading to death of MCF-7 cells. The study showed two- and three-times higher expression of the caspase-3, and − 7 genes respectively in MCF-7 cells treated with chicken peptides (especially cathelicidin-2 and − 3) relative to untreated cells which encouraged pro-apoptotic pathway, autophagy, and augmentation of the anti-proliferative activity. Our data showed that chicken ( CATH-1 ) enhance releasing of TNFα, INF-γ and upregulation of granzyme K in treated mice groups, in parallel, the tumor size and volume was reduced in the treated EAC-bearing groups after cathelicidin administration compared to untreated EAC-bearing group. Additionally, animals received high dose of cathelicidin-1 (40 µg/ml) displayed an apical survival rate compared to untreated carcinoma control and animals which received low dose of cathelicidin (10 and 20 µg/ml). Tumor of mice groups treated with chicken cathelicidin displayed high area of necrosis compared to untreated EAC-bearing mice. Based on histological analysis and immunohistochemical staining revealed that the tumor section in Ehrlich solid tumor exhibited a strong Bcl2 expression in untreated control compared to mice treated with 10 & 20 µg/ml of cathelicidin. Interestingly, low expression of Bcl2 were observed in mice taken 40 µg/ml of CATH-1. This study drive intention in treatment of cancer through the efficacy of anticancer efficacy of chicken cathelicidin peptides.

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“…Furthermore, it has also been determined that defensins, such as αand β-defensins, have antiviral activity against IAV (Doss et al, 2009). Extensive studies have been carried out on chicken cathelicidins, which have been found to exhibit various activities (Peng et al, 2020). In addition to broad-spectrum antibacterial activity, they also possessed immunomodulatory functions; hence, they may be able to activate phagocytosis, neutralize LPS-induced immune responses and improve DNA-induced TLR21 activation (Veldhuizen et al, 2013;Coorens et al, 2015;Coorens et al, 2017, Peng et al, 2020.…”

Section: Discussionmentioning

confidence: 99%

The antimicrobial potentialities of (Nk-lysin peptides of chicken, bovine, and human) against bacteria and rotavirus

Mahmoud¹,

Al-Hejin²,

Abujaml³

et al. 2021

Preprint

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For the first time, this study was carried out to investigate and evaluate the relative antibacterial activity of three different Nk-lysin peptides from human, chicken, and bovine activity compared to Gram-negative and Gram-positive bacteria as well as antiviral activity against rotavirus (strain SA-11) and finally mechanisms of action optionality. This report is the first of its kind that investigates the increased antimicrobial ability of (Nk-lysin + AgNPs) and (Nk-lysin + human IL-2) combinations against S. typhi activity by carrying out direct comparison under similar experimental settings. Our results showed that gram-negative and gram-positive microorganisms, including Streptococcus pyogenes, Streptococcus mutans, Escherichia coli, Pseudomonas aeruginosa, Klebsiella oxytoca, Shigella sonnei, Klebsiella pneumoniae and Salmonella typhimurium, are susceptible to NK-lysin treatment. It was shown in our findings that there was equal potentiality in mixture (Nk-lysin + AgNPs) and (Nk-lysin + human IL-2) for preventing the growth of S. typhi, however, when added together, there was minor increase in the level of action. In our study, the TOHO-1 gene was absent in treated bacteria. Following treatment with Nk-lysin peptides, the beta-lactamases genes (CTX-M-1, M-8, and M-9) were not found in any bacterial strains. The examination did not find any of the plasmid mediated quinolone resistance genes in the bacterial strains as a response to NK-lysin treatment. Nonetheless, no study has been carried out in the past that characterized the antiviral activity of bovine, human and chicken Nk-lysin peptides. Hence, this is the foremost study on the enhanced antimicrobial activity of human, bovine and chicken Nk-lysin peptides against Rotavirus (strain SA-11). The findings of the study demonstrated that the powerful antiviral activities were exhibited by Nk-lysin peptides against Rotavirus (strain SA-11). Based on the comparison between these peptides, it can be concluded that there is an evident potent antiviral activity of bovine Nk-lysin against Rotavirus (strain SA-11) as it restrains infection by up to 90%. However, growth was restricted by 80% by chicken Nk-lysin and by 50% by the human peptide.

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Antiviral Activity of Chicken Cathelicidin B1 Against Influenza A Virus

Cited by 23 publications

References 36 publications

The immunomodulatory effect of cathelicidin-B1 on chicken macrophages

The immunomodulatory effect of cathelicidin-B1 on chicken macrophages

Anticancer activity of chicken cathelicidin peptides against different types of cancer

The antimicrobial potentialities of (Nk-lysin peptides of chicken, bovine, and human) against bacteria and rotavirus

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