The chicken oviduct is a unique organ in which ovulated yolk transforms into a complete egg. Ovarian hormones induce the cellular and biochemical changes in the oviducts during the egg formation and oviposition. Estradiol regulates the folliculogenesis, accumulation of yolk in the follicles, ovulation, and development of oviducts. Estradiol also induces glandular development and expression of the genes responsible for egg white proteins. Progesterone induces the ovulation of yolk from the ovary, and development of oviductal glands. In addition, several genes are spatiotemporally expressed in the magnum for albumen synthesis and deposition around the yolk, in the isthmus for shell membranes synthesis, and in the uterus for eggshell biomineralization. This chapter highlights the involvement of hormones, genes/proteins, and their interaction for egg formation in the oviduct of laying hens. different segment of oviducts help in the cellular remodeling, secretion, synthesis and transport of essential molecules for the egg formation. Understanding of this information will be helpful in developing persistence layers with quality eggs.