Antiviral activity of selected cathelicidins against infectious bronchitis virus

Verheije, Monique H.; Coorens, Maarten; Weerts, E.A.W.S.; Berends, Alinda J.; Harten, Roel M. van; Angel, Marloes; Kooij, Jannetje; Ordoñez, Soledad R.; Beurden, Steven J. van; Dijk, Albert van; Haagsman, Henk P.; Veldhuizen, Edwin J.A.

doi:10.1002/pep2.24234

Cited by 4 publications

(2 citation statements)

References 40 publications

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“…In IAV and RSV, LL-37 produced notable disruption of virions under electron microscopy 19 , 21 . For IBV, no virion disruption was observed by EM and the inhibitory effect was cell-type specific, suggesting an effect on other components of viral entry, such as the viral spike or host entry receptor 18 .…”

Section: Introductionmentioning

confidence: 93%

“…Early experiments with LL-37 and murine CRAMP cathelicidin peptides observed inhibition of multiple influenza A virus (IAV) strains both in cell culture and in murine infection experiments 17 . Later experiments with IAV, respiratory syncytia virus (RSV), and infectious bronchitis virus (IBV) indicated that LL-37 directly affects IAV, RSV, and IBV virions during cellular infection, as LL-37 added at the time of infection effectively inhibited either virus, but not when added to cells pre- or post-infection 18 – 20 . The lack of inhibition observed in pre-treated cells suggests that a minority of the inhibition is dependent on conditioning of host cells and that the majority of LL-37 is consumed within a few hours of addition.…”

Section: Introductionmentioning

confidence: 99%

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A wild boar cathelicidin peptide derivative inhibits severe acute respiratory syndrome coronavirus-2 and its drifted variants

von Beck,

Navarrete,

Arce

et al. 2023

Sci Rep

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The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) poses a clear threat to humanity. It has infected over 200 million and killed 4 million people worldwide, and infections continue with no end in sight. To control the pandemic, multiple effective vaccines have been developed, and global vaccinations are in progress. However, the virus continues to mutate. Even when full vaccine coverage is achieved, vaccine-resistant mutants will likely emerge, thus requiring new annual vaccines against drifted variants analogous to influenza. A complimentary solution to this problem could be developing antiviral drugs that inhibit SARS CoV-2 and its drifted variants. Host defense peptides represent a potential source for such an antiviral as they possess broad antimicrobial activity and significant diversity across species. We screened the cathelicidin family of peptides from 16 different species for antiviral activity and identified a wild boar peptide derivative that inhibits SARS CoV-2. This peptide, which we named Yongshi and means warrior in Mandarin, acts as a viral entry inhibitor. Following the binding of SARS-CoV-2 to its receptor, the spike protein is cleaved, and heptad repeats 1 and 2 multimerize to form the fusion complex that enables the virion to enter the cell. A deep learning-based protein sequence comparison algorithm and molecular modeling suggest that Yongshi acts as a mimetic to the heptad repeats of the virus, thereby disrupting the fusion process. Experimental data confirm the binding of Yongshi to the heptad repeat 1 with a fourfold higher affinity than heptad repeat 2 of SARS-CoV-2. Yongshi also binds to the heptad repeat 1 of SARS-CoV-1 and MERS-CoV. Interestingly, it inhibits all drifted variants of SARS CoV-2 that we tested, including the alpha, beta, gamma, delta, kappa and omicron variants.

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Section: Introductionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

A wild boar cathelicidin peptide derivative inhibits severe acute respiratory syndrome coronavirus-2 and its drifted variants

von Beck,

Navarrete,

Arce

et al. 2023

Sci Rep

View full text Add to dashboard Cite

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Immunomodulatory properties of chicken cathelicidin-2 investigated on an ileal explant culture

Mátis,

Tráj,

Hanyecz

et al. 2024

Vet Res Commun

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As the threat posed by antimicrobial resistance grows more crucial, the development of compounds that can replace antibiotics becomes increasingly vital. Chicken cathelicidin-2 (Cath-2) belongs to the group of Host Defense Peptides (HDPs), which could provide a feasible solution for the treatment of gastrointestinal infections in poultry. It is a small peptide produced by the heterophil granulocytes of chickens as part of the innate immune response, and its immunomodulatory activity has already been demonstrated in several cell types. In this study, the effects of Cath-2 on the intestinal immune response were examined using ileal explant cultures isolated from chicken. Regarding our results, Cath-2 displayed a potent anti-inflammatory effect as it alleviated the LTA-caused elevation of interleukin (IL)-6 and IL-2 concentrations, and that of the IFN-γ/IL-10 ratio, furthermore, it increased the concentration of IL-10, alleviating the LTA-evoked decreased level of the anti-inflammatory cytokine. Moreover, when applied alone, it elevated the concentrations of IL-6, CXCLi2, and IL-2, providing evidence of its complex immunomodulatory mechanisms. In summary, Cath-2 was able to modulate the immune response of the intestinal wall not only by reducing pro-inflammatory cytokine release, but also through immune stimulation, demonstrating that it has the ability to improve innate immunity via a complex mechanism that may make it a suitable candidate for the control of intestinal infections.

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Application of antimicrobial chitosan-Furcellaran-hydrolysate gelatin edible coatings enriched with bioactive peptides in shelf-life extension of pork loin stored at 4 and −20 °C

Kulawik,

Jamróz,

Tkaczewska

et al. 2024

International Journal of Biological Macromolecules

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Antiviral activity of selected cathelicidins against infectious bronchitis virus

Cited by 4 publications

References 40 publications

A wild boar cathelicidin peptide derivative inhibits severe acute respiratory syndrome coronavirus-2 and its drifted variants

A wild boar cathelicidin peptide derivative inhibits severe acute respiratory syndrome coronavirus-2 and its drifted variants

Immunomodulatory properties of chicken cathelicidin-2 investigated on an ileal explant culture

Application of antimicrobial chitosan-Furcellaran-hydrolysate gelatin edible coatings enriched with bioactive peptides in shelf-life extension of pork loin stored at 4 and −20 °C

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