Antiviral activity of seselin from Aegle marmelos against nuclear polyhedrosis virus infection in the larvae of silkworm, Bombyx mori

Somu, Chitra; Hilda, Karuppiah; Janarthanan, Sundaram

doi:10.1016/j.jep.2019.112155

Cited by 19 publications

(8 citation statements)

References 33 publications

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“…Our findings are aligned with the previous research on DE circRNAs in midgut following BmNPV infection (Hu, Zhu, Liu, et al, 2018; Hu, Zhu, Zhang, et al, 2018). It is reported that the reproduction of BmNPV is highly dependent on host energy metabolism (Somu, Karuppiah, & Sundaram, 2019; Wu, Shang, Huang et al, 2019; Yu et al, 2017). The results revealed that those DE circRNAs affected metabolic balance during the process of virus infection.…”

Section: Discussionmentioning

confidence: 99%

Expression profile analysis of circular RNAs in BmN cells (Bombyx mori) upon BmNPV infection

Zhang

Shen

Yin

et al. 2020

Arch Insect Biochem Physiol

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The disease caused by Bombyx mori nucleopolyhedrovirus (BmNPV) has always been difficult to control, resulting in tremendous economic losses in the sericulture industry. Although much has been learned about the impact of noncoding RNAs on pathogenesis, the role of circular RNA (circRNA) in insect immunity remains unclear. To explore circRNA regulation involved in BmNPV infection, we used transcriptome analysis of BmN cells with or without BmNPV infection to generate circRNA data set. A total of 444 novel circRNAs were identified in BmN cells, with 198 pervasively distributed both in the control group and BmNPV-infection group. The host genes were enriched inMAPK signaling pathway, dorso-ventral axis formation, and ECM-receptor interaction, which were required for the normal larval growth. A total of 75 circRNAs were differentially expressed (DE) on BmNPV infection. Six downregulated circRNAs were validated by Sanger sequencing and qRT-PCR. DEcircRNA-miRNA-DEmRNA network was constructed based on the six validated cir-cRNAs. Pathway analysis indicated that the predicted target genes were mainly enriched in the metabolic pathway and immune-related signaling pathway. Our results may provide a basis for further studies on circRNA function in BmN cells challenged by BmNPV infection and offer an

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Section: Discussionmentioning

confidence: 99%

Expression profile analysis of circular RNAs in BmN cells (Bombyx mori) upon BmNPV infection

Zhang

Shen

Yin

et al. 2020

Arch Insect Biochem Physiol

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“…Interestingly, a high throughput screening of approximately 6800 small molecules identi ed that proteases of picornavirus and coronavirus have a common inhibitors [16]. Importantly, in our earlier report, we have successfully puri ed the molecule seselin from the leaf extracts of A. marmelos [17]. It is noteworthy that it exhibited anti-viral activity against Bombyx mori nuclear polyhedrosis virus (BmNPV).…”

Section: Introductionmentioning

confidence: 95%

“…The structure of the ligand seselin, an anti-BmNPV compound obtained from the puri ed crystal in our earlier report [17] was drawn using MarvinSketch program (MarvinSketch, ChemAxon). PRODRG server was used to describe the ligand molecules and a variety of topologies for use with GROMACS, WHAT IF, Autodock, HEX etc., were generated.…”

Section: Preparation Of Ligandmentioning

confidence: 99%

Inhibition of multiple SARS-CoV-2 proteins by an antiviral biomolecule, seselin fromAegle marmelosdeciphered using molecular docking analysis

Nivetha

Bhuvaragavan

Thirunavukkarasu

et al. 2021

Journal of Biomolecular Structure and Dynamics

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Our earlier experimental and computational report produced the evidence on anti-viral nature of the compound seselin puri ed from the leaf extracts of Aegle marmelos against Bombyx mori Nuclear Polyhedrosis Virus (BmNPV). In the pandemic situation of COVID-19 caused by SARS-COV-2 virus, an in silico effort to evaluate the potentiality of the seselin has been made to test its e cacy against multiple targets of SARS-COV-2 such as SARS-CoV-2S spike protein, COVID-19 main protease and free enzyme of the SARS-CoV-2 (2019-nCoV) main protease. The ligand, seselin showed the best interaction with receptors SARS-CoV-2S protein, COVID-19 main protease and free enzyme of the SARS-CoV-2 (2019-nCoV) main protease with a binding energy of -6.6 kcal/mol, -6.9 kcal/mol and -6.7 kcal/mol, respectively. Docking analysis with three different receptors identi ed that all the computationally predicted lowest energy complexes were stabilized by intermolecular hydrogen bonds and stacking interactions. The aminoacid residues involved in interactions are THR111 and GLN110 for spike protein, SER1003, ALA958 and THR961 for COVID-19 main protease, and for SARS-CoV-2 (2019-nCoV) main protease, it is THR111, GLN110 and THR292. The outcome of pharmacokinetic analysis suggests that the compound had favourable drugability properties by obeying Lipinski rule of ve with e cient ADME properties and exhibiting high a nity towards the binding site that it was directed to. The results suggest that the seselin has inhibitory potential over multiple SARS-COV-2 targets and holds a high potential to work effectively as a novel drug for COVID-19, if evaluated in experimental set ups in foreseeable future.

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“…[48][49][50] The Aegle marmelos being an important medicinal plant with several bioactive compounds such as Seselin, Aeglein, Marmelide, and Marmelosin is chosen for antiviral study against SARS-CoV-2. [51][52][53][54][55] Some Phyllanthus species also exhibit inhibitory potential against HBV, HCV, HIV, and HSV [56][57][58] and Phyllanthus emblica against respiratory syndrome virus (PRRSV). 59 Chebulagic Acid, Pedunculagin, Azadirachtin, and Nimbolide may act as potential inhibitors of the SARS-CoV-2 ACE2 receptor and M pro .…”

Section: Introductionmentioning

confidence: 99%

In Silico Exploration of Phytoconstituents From Phyllanthus emblica and Aegle marmelos as Potential Therapeutics Against SARS-CoV-2 RdRp

Pandey

Lokhande

Swamy

et al. 2021

Bioinform Biol Insights

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) worldwide has increased the importance of computational tools to design a drug or vaccine in reduced time with minimum risk. Earlier studies have emphasized the important role of RNA-dependent RNA polymerase (RdRp) in SARS-CoV-2 replication as a potential drug target. In our study, comprehensive computational approaches were applied to identify potential compounds targeting RdRp of SARS-CoV-2. To study the binding affinity and stability of the phytocompounds from Phyllanthus emblica and Aegel marmelos within the defined binding site of SARS-CoV-2 RdRp, they were subjected to molecular docking, 100 ns molecular dynamics (MD) simulation followed by post-simulation analysis. Furthermore, to assess the importance of features involved in the strong binding affinity, molecular field-based similarity analysis was performed. Based on comparative molecular docking and simulation studies of the selected phytocompounds with SARS-CoV-2 RdRp revealed that EBDGp possesses a stronger binding affinity (−23.32 kcal/mol) and stability than other phytocompounds and reference compound, Remdesivir (−19.36 kcal/mol). Molecular field-based similarity profiling has supported our study in the validation of the importance of the presence of hydroxyl groups in EBDGp, involved in increasing its binding affinity toward SARS-CoV-2 RdRp. Molecular docking and dynamic simulation results confirmed that EBDGp has better inhibitory potential than Remdesivir and can be an effective novel drug for SARS-CoV-2 RdRp. Furthermore, binding free energy calculations confirmed the higher stability of the SARS-CoV-2 RdRp-EBDGp complex. These results suggest that the EBDGp compound may emerge as a promising drug against SARS-CoV-2 and hence requires further experimental validation.

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Antiviral activity of seselin from Aegle marmelos against nuclear polyhedrosis virus infection in the larvae of silkworm, Bombyx mori

Cited by 19 publications

References 33 publications

Expression profile analysis of circular RNAs in BmN cells (Bombyx mori) upon BmNPV infection

Expression profile analysis of circular RNAs in BmN cells (Bombyx mori) upon BmNPV infection

Inhibition of multiple SARS-CoV-2 proteins by an antiviral biomolecule, seselin fromAegle marmelosdeciphered using molecular docking analysis

In Silico Exploration of Phytoconstituents From Phyllanthus emblica and Aegle marmelos as Potential Therapeutics Against SARS-CoV-2 RdRp

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