Antiviral activity of Taurisolo® during bovine alphaherpesvirus 1 infection

Fungal metabolite 6-pentyl-α-pyrone reduces canine coronavirus infection

“…In order to check cell proliferation, MTT assay was carried out [ [32] , [33] , [34] , [35] ]. In a first test of cell viability, five concentrations of 6 PP (0.001, 0.01, 0.1, 1 and 10 μg/mL) were checked at 48 h p. i., and then we have chosen 0.1 μg/mL 6 PP for MTT test.…”

“…RT-qPCR was used for quantifying CCoV during infection in A72 cells [ 39 ]. Then, the quantification of CCoV was carried by the mean of a standard curve, making serial dilutions (from 3.5 × 10 9 to 3.5 × 10 2 TCID 50 /mL) of the quantified canine coronavirus viral stock and plotting threshold cycle number (Ct) against the TCID 50 /mL on a log scale [ 14 , 16 , 17 , 22 , 35 ].…”