Antiviral activity of the dichloromethane extracts from  Artocarpus heterophyllus  leaves against hepatitis C virus

Hafid, Achmad Fuad; Aoki-Utsubo, Chie; Permanasari, Adita Ayu; Adianti, Myrna; Tumewu, Lydia; Widyawaruyanti, Aty; Wahyuningsih, Sri Puji Astuti; Wahyuni, Tutik Sri; Lusida, Maria Inge; Soetjipto,; Hotta, Hak

doi:10.1016/j.apjtb.2017.06.003

Cited by 37 publications

(27 citation statements)

References 27 publications

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“…HCV supernatants were harvested on day 3 and were concentrated through an Amicon Ultra-15 centrifugal filter (Millipore) by centrifugation at 3500 rpm, 15 min, 4 o C. DAB staining was used to visualize infected cells for virus titration. 7…”

Section: Hcv Propagationmentioning

confidence: 99%

The Alkaloid Fraction from Melicope latifolia Leaves Inhibits Hepatitis C Virus

Susiloningrum¹,

Permanasari²,

Adianti³

et al. 2020

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the treatment need to be considered as important problems. 4,5 In order to circumvent those problems, the development of new antiviral drugs is very much required. Some plants have been reported to have hepatitis C antiviral activity. Plant extracts from Toona sureni, 6 Artocarpus heterophyllus, 7 Ficus fistulosa, 8 Alectron serratus, 9 and Melicope latifolia 4 significantly inhibited HCV activity in vitro. A variety of active phytochemicals such as flavonoids, terpenoids, lignins, sulphides, polyphenolics, coumarins, saponins, furyl, alkaloids, polylines, thiophenes, protein, and peptides have been identified to inhibit various viruses. 10 Melicope latifolia belongs to the Rutaceae family, commonly known in Indonesia as Ki Sampang and is traditionally used to cure fever and abdominal cramps. The tree typically has a height of about 20 m and is commonly found in primary and secondary open forests. M. latifolia plants are distributed in Malaysia, Philippines, Java-Indonesia, Kalimantan-Indonesia and Papua New Guinea. 11 The chemical compounds which were contained in the M. latifolia plant, are flavonoids, alkaloid, coumarins and terpenoids. 10,12 In general, plant species in the Rutaceae family contain the chemical constituent of alkaloid group, including the Melicope genus.

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Section: Hcv Propagationmentioning

confidence: 99%

The Alkaloid Fraction from Melicope latifolia Leaves Inhibits Hepatitis C Virus

Susiloningrum¹,

Permanasari²,

Adianti³

et al. 2020

Self Cite

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“…Culture supernatant from the infected cells was collected at day 2 and day 5 post infection and concentrated using Amicon Ultra centrifugal filter unit. Virus titers were determined for antiviral assay [28,29]. Antiviral activity assay: Antiviral activity assay was conducted as described previously [7,8,28,29].…”

Section: Cells and Virusesmentioning

confidence: 99%

“…Virus titers were determined for antiviral assay [28,29]. Antiviral activity assay: Antiviral activity assay was conducted as described previously [7,8,28,29]. Huh7it cells (5.4 x 10 4 ) were seeded for 24 hours.…”

Section: Cells and Virusesmentioning

confidence: 99%

Antiviral Activities of Curcuma Genus against Hepatitis C Virus

Wahyuni

Permatasari

Widiandani

et al. 2018

Natural Product Communications

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Hepatitis C virus (HCV) infection is one of the major public health problems in the world. Even though the new agents are shown to increase the sustained virology response, however, there are still many people who cannot access the therapy due to the high cost. Moreover, the emergence of resistance and side effects presented the necessity to develop alternative treatment agents for HCV infection. Plants of the genus of curcuma are popular among traditional medicines in the world, including Indonesia. They have been used for many herb remedies and reported to possess many biological activities. Several plants from the curcuma genus were known as treatment agents in liver disease and jaundice. Our current study determines antiviral activities of Curcuma domestica, Curcuma xanthorrhiza, and Curcuma heyneana against HCV and further examines the mechanism of actions. Antiviral activity was performed by in vitro culture cells using Huh 7.5it cells and treated with the mixture of extract and virus JFH1. The effects of extracts in HCV life cycle were determined by mode of action analysis to examine the action of substances in the entry or post entry steps. The results revealed that ethanol extract of C. domestica, C. xanthorrhiza, and C. heyneana showed strong anti-HCV activities with IC 50 values of 1.68 ± 0.05, 4.93 ± 0.42 and 5.49 ± 0.59 µg/mL, respectively without any cytotoxicity effect. Mode of action analysis demonstrated that of C. domestica and C. heyneana exhibit HCV in the entry step, while C. xanthorrhiza inhibit in the entry and post entry steps of HCV life cycle. Docking analysis to predict the interaction of curcumin, the main compound of curcuma genus, revealed a strong interaction between curcumin and 4GAG receptor, a protein involved in the entry step of HCV infection. Moreover, it was also reported to possess good interaction with 4EAW, an HCV NS5B, which plays an important role in HCV replication. These results suggested that C. domestica, C. xanthorrhiza, and C. Heyneana possessed strong inhibition against hepatitis C virus, therefore they may be good candidates for anti-HCV agents.

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“…Crude extracts of various parts of the plant exhibited broad-spectrum antibacterial activity [3,6,7], including activity against cariogenic bacteria [8] and multidrug resistant Staphylococcus aureus [9]. A jackfruit seed-derived lectin, Jacalin, inhibits DNA viruses such as herpes simplex virus type II, varicella-zoster virus, and cytomegalovirus, and crude extracts from leaves have anti-hepatitis C virus activity [10,11]. Several investigators have reported the antioxidant activity of extracts from defatted jackfruit seeds, pulp, and dried mature fruits in in vitro assays [5].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Water-Soluble Polysaccharide from Jackfruit (Artocarpus heterophyllus Lam.) on Human Colon Carcinoma Cells Cultured In Vitro

Wiater

Paduch

Trojnar

et al. 2020

Plants

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Various phytochemical studies have revealed that jackfruit (Artocarpus heterophyllus Lam.) is rich in bioactive compounds, including carotenoids, flavonoids, volatile acids, tannins, and lectins. The aim of the study was to analyze the biological activity of water-soluble polysaccharide (WSP) isolated from jackfruit and to assess its immunomodulatory, cytotoxic, and anti-oxidative effects on human colon carcinoma cells in vitro. The neutral red (NR) uptake assay revealed no toxic influence of the polymer on the viability of tumor cells (HT29 and SW620). After 24 h and 48 h of incubation, the cellular viability was not lower than 94%. The metabolic activity of the cells (MTT) at the compound concentration of 250 µg/mL was higher than 92% in comparison to the control. WSP (250 µg/mL) exerted no significant effect on the morphology of the cells was determined by May-Grünwald-Giemsa staining. WSP changed nitric oxide (NOx) production by the tumor cells depending on the time of incubation and prior 2-h stimulation of the cells with E. coli 0111:B4 LPS. It significantly stimulated IL-1β production by the tumor cells. The IL-6 level increased but that of IL-10 decreased by a WSP concentration-dependent manner. No such effect was detected in SW620. The WSP had antioxidant properties. In conclusion, water-soluble polysaccharide isolated from A. heterophyllus exhibits significant biological activity towards many types of both normal and cancerous cells. Therefore, it may be considered as a useful agent in the protection of human health or in functional and dietary nutrition.

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Antiviral activity of the dichloromethane extracts from Artocarpus heterophyllus leaves against hepatitis C virus

Cited by 37 publications

References 27 publications

The Alkaloid Fraction from Melicope latifolia Leaves Inhibits Hepatitis C Virus

The Alkaloid Fraction from Melicope latifolia Leaves Inhibits Hepatitis C Virus

Antiviral Activities of Curcuma Genus against Hepatitis C Virus

The Effect of Water-Soluble Polysaccharide from Jackfruit (Artocarpus heterophyllus Lam.) on Human Colon Carcinoma Cells Cultured In Vitro

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