Antiviral activity on the Zika virus and larvicidal activity on the Aedes spp. of Lippia alba essential oil and β-caryophyllene

Sobrinho, Antonio Carlos Nogueira; Morais, Selene Maia de; Marinho, Márcia Machado; Souza, Natália Vasconcelos de; Lima, Danielle Malta

doi:10.1016/j.indcrop.2021.113281

Cited by 39 publications

(14 citation statements)

References 42 publications

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“…EOs also have intracellular modes of action by blocking the viral particle formation and releasing them from the host cell [ 12 , 13 , 14 ]. EO of L. alba is rich in trans- β-caryophyllene and this isolated sesquiterpene reduced the Zika virus replication, in an enveloped virus similar in structure to DENV, by treatment after virus adsorption to the cell [ 49 ]. We could hypothesize that inactive or weakly active EOs in this study, might exhibit strong anti-DENV activity in a cell-based assay that examines the inhibitory effect after virus adsorption to cells.…”

Section: Discussionmentioning

confidence: 99%

Essential Oils from Colombian Plants: Antiviral Potential against Dengue Virus Based on Chemical Composition, In Vitro and In Silico Analyses

et al. 2022

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Currently, there are no therapies to prevent severe dengue disease. Essential oils (EOs) can serve as primary sources for research and the discovery of phytomedicines for alternative therapy. Fourteen EOs samples were obtained by distillation from six plants used in Colombian folk medicine. GC/MS analysis identified 125 terpenes. Cytopathic effect (CPE) reduction assays revealed differences in antiviral activity. EOs of Lippia alba, citral chemotype and carvone-rich fraction; Lippia origanoides, phellandrene chemotype; and Turnera diffusa, exhibited strong antiviral activity (IC50: 29 to 82 µg/mL; SI: 5.5 to 14.3). EOs of Piper aduncum, Ocimum basilicum, and L. origanoides, carvacrol, and thymol chemotypes, exhibited weak antiviral activity (32 to 53% DENV-CPE reduction at 100 µg/mL; SI > 5.0). Cluster and one-way ANOVA analyses suggest that the strong antiviral activity of EOs could be attributed to increased amounts of non-phenolic oxygenated monoterpenes and sesquiterpene hydrocarbons. Docking analyses (AutoDock Vina) predicted binding affinity between the DENV-2 E protein and terpenes: twenty sesquiterpene hydrocarbons (−8.73 to −6.91 kcal/mol), eight oxygenated monoterpenes (−7.52 to −6.98 kcal/mol), and seven monoterpene hydrocarbons (−7.60 to −6.99 kcal/mol). This study reports for the first time differences in the antiviral activity of EOs against DENV, corresponding to their composition of monoterpenes and sesquiterpenes.

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Section: Discussionmentioning

confidence: 99%

Essential Oils from Colombian Plants: Antiviral Potential against Dengue Virus Based on Chemical Composition, In Vitro and In Silico Analyses

et al. 2022

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“…The good larvicidal activity of S. attopeuense and S. tonkinense essential oils may be related to their chemical constituents. Indeed, bicyclogermacrene, (E)-β-ocimene and (E)-caryophyllene are the main components in essential oils of S. attopeuense and S. tonkinense that have been shown to have larvicidal activity (Chau et al, 2020;Nogueira Sobrinho et al, 2021). In addition, the components in lower amounts could also contribute to the larvicidal activity of the essential oil by acting in a synergistic manner.…”

Section: Larvicidal Activity Of Essential Oilsmentioning

confidence: 99%

Chemical composition, antimicrobial and larvicidal activities of essential oils of two Syzygium species from Vietnam

et al. 2024

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The present study is the first to investigate the chemical composition, antimicrobial and larvicidal activities of the essential oils from the leaves of Syzygium attopeuense (Gagnep.) Merr. & L.M.Perry and Syzygium tonkinense (Gagnep.) Merr. & L.M.Perry collected in Vietnam. The essential oils were extracted by hydrodistillation and analyzed by GC and GC–MS. The study indicated the presence of a high percentage of sesquiterpenes in both investigated essential oils. The major components of S. attopeuense essential oil were bicyclogermacrene (24.26%), (E)-caryophyllene (11.72%), and (E)-β-ocimene (6.75%), whereas S. tonkinense essential oil was dominated by (E)-caryophyllene (80.80%). The antimicrobial activity of essential oils was evaluated by broth microdilution assay to determine the minimum inhibitory concentration (MIC) and median inhibitory concentration (IC50). Both essential oils exhibited remarkable inhibitory activity against all tested Gram-positive bacteria and yeast than Gram-negative bacteria. Among them, essential oils of S. attopeuense and S. tonkinense possessed the strongest activity against Enterococcus faecalis (MIC = 4.00 μg/mL; IC50 = 1.69 μg/mL) and Candida albicans (MIC = 16.00 μg/mL; IC50 = 8.67 μg/mL), respectively. Furthermore, the larvicidal activity of essential oils was tested using fourth-instar larvae of Aedes aegypti. Results from the larvicidal test revealed that both essential oils had an excellent inhibitory effect against A. aegypti larvae with LC50 values from 25.55 to 30.18 μg/mL and LC90 values from 33.00 to 39.01 μg/mL. Our findings demonstrate that the essential oil extracted from S. attopeuense and S. tonkinense are potential sources of natural antimicrobials and can act as inexpensive mosquito larvicidal agents.

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“…The chemical analysis of the oil of MO and GMM constituents was performed according to the method suggested by Sobrinho and team using an Agilent 8890 GC for gas chromatography and 59777B GC/MSD for mass spectrometry with slight modi cations [21]. Column: Rtx-5MS (Cross bond, 5% diphenyl/95% dimethyl polysiloxane) measuring 30m x0.…”

Section: Gas Chromatography-mass Spectrometry (Gcms) Analysismentioning

confidence: 99%

Volatile compounds from garlic (Allium sativum L.) mustard (Brassica nigra L.) oil macerate enhances the activity of multiple antibiotics against Staphylococcus aureus: A In-vitro and in silico study.

Singha,

Dutta,

Saikia

2023

Preprint

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Staphylococcus aureus is known to be associated with various human infections worldwide. Due to an increase in antibiotic resistance, S. aureus infections are difficult to cure with the present generation of antibiotics. Therefore, new antibacterial compounds or adjuvant discovery is in demand at present. In the present research garlic mustard oil macerate is prepared by heating garlic cloves macerated in mustard oil for 4 hours at 80 ºC. The effect of the macerate vapour on the antibacterial activity of gentamycin, kanamycin, and streptomycin against S. aureus was examined and found to be significantly increased compared to antibiotics alone. GMM vapour inhibited the staphyloxanthin production by 44.237±0.14 % compared to mustard oil vapour treated bacterial cells. The bacterial cell membrane disruption was seen during SEM analysis on the bacterial cells treated with GMM vapour. LCMS and GCMS analysis confirmed the presence of 2-vinyl-4H-1,3, dithiin, ajoene, allicin, sinigrin, and allyl isothiocyanate (AITC). In-silico molecular docking analysis has shown hydrophobic and Van der Waals interaction of the phytochemicals with dehydroxysqualene synthase protein (crtM) of S. aureus. However, allicin and sinigrin had also shown hydrogen bonding with crtM proteins.

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Antiviral activity on the Zika virus and larvicidal activity on the Aedes spp. of Lippia alba essential oil and β-caryophyllene

Cited by 39 publications

References 42 publications

Essential Oils from Colombian Plants: Antiviral Potential against Dengue Virus Based on Chemical Composition, In Vitro and In Silico Analyses

Essential Oils from Colombian Plants: Antiviral Potential against Dengue Virus Based on Chemical Composition, In Vitro and In Silico Analyses

Chemical composition, antimicrobial and larvicidal activities of essential oils of two Syzygium species from Vietnam

Volatile compounds from garlic (Allium sativum L.) mustard (Brassica nigra L.) oil macerate enhances the activity of multiple antibiotics against Staphylococcus aureus: A In-vitro and in silico study.

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