Antiviral Effect and Mechanism of Edaravone against Grouper Iridovirus Infection

Abstract

Singapore grouper iridovirus (SGIV) is a virus with high fatality rate in the grouper culture industry. The outbreak of SGIV is often accompanied by a large number of grouper deaths, which has a great impact on the economy. Therefore, it is of great significance to find effective drugs against SGIV. It has been reported that edaravone is a broad-spectrum antiviral drug, most widely used clinically in recent years, but no report has been found exploring the effect of edaravone on SGIV infections. In this study, we evaluated the antiviral effect of edaravone against SGIV, and the anti-SGIV mechanism of edaravone was also explored. It was found that the safe concentration of edaravone on grouper spleen (GS) cells was 50 µg/mL, and it possessed antiviral activity against SGIV infection in a dose-dependent manner. Furthermore, edaravone could significantly disrupt SGIV particles and interference with SGIV binding to host cells, as well as SGIV replication in host cells. However, edaravone was not effective during the SGIV invasion into host cells. This study was the first time that it was determined that edaravone could exert antiviral effects in response to SGIV infection by directly interfering with the processes of SGIV infecting cells, aiming to provide a theoretical basis for the control of grouper virus disease.

A review: Marine aquaculture impacts marine microbial communities

“…V. parahaemolyticus phages such as F23s2, H256D1, and the recently discovered phage phiTY18 showed inhibitory effects, thus offering alternatives to antibiotics to control V. parahaemolyticus [65] , [66] . However, certain viruses, such as the white spot syndrome virus, the Singapore grouper iridovirus, and the infectious subcutaneous and hematopoietic necrosis viruses in aquaculture, exhibit a high fatality rate, thus posing a severe threat to crustaceans and groupers and hindering economic development [67] – [69] .…”