Antiviral effect of catechins in green tea on influenza virus

Song, Jae Min; Lee, Kwang Hee; Seong, Baik Lin

doi:10.1016/j.antiviral.2005.06.010

Cited by 573 publications

(426 citation statements)

References 21 publications

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“…Thereby, it is conceivable that the inhibitory effect of PGG was mainly caused by the direct interaction of PGG with virus. Numerous studies have demonstrated that plant polyphenols including the tea catechins (-) epigallocatechin gallate (EGCG) and theaflavin digallate [34], resveratrol (RV) [26], a polyphenol rich extract (CYSTUS052) [11], pomegranate polyphenol extract (PPE) [13] , oligonol [12], and Hydroxytyrosol (HT) [38], have potent antiviral activity against influenza virus, that are related to the nature of their interactions with viral particles. Additionally, indirect effects of some polyphenols, such as EGCG and strictinin, on host cells that might interfere with virus-cell membrane fusion have also been suggested [23,32].…”

Section: Discussionmentioning

confidence: 99%

Antiviral activity and possible mechanisms of action of pentagalloylglucose (PGG) against influenza A virus

et al. 2011

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Influenza A virus (IAV) infection is a major public health threat leading to significant morbidity and mortality. The emergence of drug-resistant virus strains highlights urgent needs to develop novel antiviral drugs with alternative modes of action. Pentagalloyl glucose (PGG), a natural occuring polyphenolic compound, possesses broad spectrum of biological activities. In this study, we found PGG has anti-influenza virus activity and investigated its possible mechanism(s) of action in vitro.

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Section: Discussionmentioning

confidence: 99%

Antiviral activity and possible mechanisms of action of pentagalloylglucose (PGG) against influenza A virus

et al. 2011

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“…Briefly, supernatants were serially diluted 10-fold and incubated with an equal volume of 1% guinea pig red blood cell suspension. After incubation, viral titers were calculated as previously described [17] .…”

Section: Hemagglutination Assaymentioning

confidence: 99%

“…Virus growth inhibition assay Measurement of virus growth inhibition was performed to determine the cytoprotective effect of EGCG, as described elsewhere [17] . Cell culture plates with 96-wells were used, and monolayers of one-day-old MDCK cells were infected with 100 median tissue culture infective dose (TCID 50 ) of influenza A virus.…”

Section: Cells Chemicals and Virus Stocksmentioning

confidence: 99%

Amelioration of influenza virus-induced reactive oxygen species formation by epigallocatechin gallate derived from green tea

Ling

Wei

et al. 2012

Acta Pharmacol Sin

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Aim: To study whether epigallocatechin gallate (EGCG), a green tea-derived polyphenol, exerted anti-influenza A virus activity in vitro and in vivo. Methods: Madin-Darby canine kidney (MDCK) cells were tested. The antiviral activity of EGCG in the cells was determined using hemagglutination assay and qPCR. Time of addition assay was performed to determine the kinetics of inhibition of influenza A by EGCG. The level of reactive oxygen species (ROS) were determined with confocal microscopy and flow cytometry. BALB/c mice were treated with EGCG (10, 20 or 40 mg·kg -1 ·d -1 , po) for 5 d. On the 3rd d of the treatment, the mice were infected with influenza A virus. Histopathological changes, lung index and virus titers in the lungs were determined. Results: Treatment of influenza A-infected MDCK cells with EGCG (1.25-100 nmol/L) inhibited influenza A replication in a concentration-dependent manner (the ED 50 value was 8.71±1.11 nmol/L). Treatment with EGCG (20 nmol/L) significantly suppressed the increased ROS level in MDCK cells following influenza A infection. In BALB/c mice infected with influenza virus, oral administration of EGCG (40 mg·kg -1 ·d -1 ) dramatically improved the survival rate, decreased the mean virus yields and mitigated viral pneumonia in the lungs, which was equivalent to oral administration of oseltamivir (40 mg·kg -1 ·d -1 ), a positive control drug. Conclusion:The results provide a molecular basis for development of EGCG as a novel and safe chemopreventive agent for influenza A infection.

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“…The inhibitory effect did not relate to the virus attachment to MDCK cells because MOEO was inoculated to the cells after virus absorption phase (1 h post-infection). Song et al [31] showed that high concentration of catechins from green tea such as epigallocatechin gallate suppress viral RNA synthesis and neuraminidase activity of Influenza viruses. Influenza virus proliferation was also inhibited by pomegranate components such as punicalagin [14].…”

Section: Discussionmentioning

confidence: 99%

Antiviral activity of the oseltamivir and Melissa officinalis L. essential oil against avian influenza A virus (H9N2)

et al. 2016

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Lemon balm derivatives are going to acquire a novelty as natural and potent remedy for treatment of viral infections since the influenza viruses are developing resistance to the current antivirals widely. Oseltamivir, Melissa officinalis essential oil (MOEO) and their synergistic efficacy against avian influenza virus (AIV) subtype H9N2 were evaluated in vitro in MDCK cells at different time exposure by using TCID50, HA, Real Time PCR and HI assay. The results showed that MOEO could inhibit replication of AVI through the different virus replication phase (P B 0.05). Also the highest antiviral activity of MOEO was seen when AIV incubated with MOEO before cell infection. The TCID50/ml was reduced 1.3-2.1, 2.3-2.8, 3.7-4.5 log 10 than control group (5.6 log 10), HAU/50 ll was decreased 85-94, 71.4-94, 71.4-94 % and viral genome copy number/ll was brought down 68-95, 90-100, 89.6-99.9 % at pre-infection, post-infection and simultaneous stage, respectively. Hemagglutination inhibition result showed the MOEO was not able to inhibit agglutination of the chicken red blood cell (cRBC). Replication of the AVI was suppressed by the different concentration of oseltamivir completely or near 100 %. Also oseltamivir showed a synergistic activity with MOEO especially when oseltamivir concentration reduced under 0.005 mg/ml. The chemical composition was examined by GC-MS analysis and Its main constituents were identified as monoterpenaldehydes citral a, citral b. In conclusion, the findings of the study showed that lemon balm essential oil could inhibit influenza virus replication through different replication cycle steps especially throughout the direct interaction with the virus particles.

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Antiviral effect of catechins in green tea on influenza virus

Cited by 573 publications

References 21 publications

Antiviral activity and possible mechanisms of action of pentagalloylglucose (PGG) against influenza A virus

Antiviral activity and possible mechanisms of action of pentagalloylglucose (PGG) against influenza A virus

Amelioration of influenza virus-induced reactive oxygen species formation by epigallocatechin gallate derived from green tea

Antiviral activity of the oseltamivir and Melissa officinalis L. essential oil against avian influenza A virus (H9N2)

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