Antiviral effects of Ma Huang Tang against H1N1 influenza virus infection in vitro and in an ICR pneumonia mouse model

Wei, Wenyang; Wan, Haitong; Peng, Xue-qian; Zhou, Huifen; Lu, Yi‐Yu

doi:10.1016/j.biopha.2018.03.161

Cited by 19 publications

(18 citation statements)

References 30 publications

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“…Lung index refers to the percentage of lung weight to body weight and can indicate the severity of pneumonia. Influenza virus infection causes viral pneumonia in mice, and consequently, inflammation exudation increases lung weight (Wei et al, 2018). Figure 1B demonstrates that the mean lung index of the model group significantly increased compared with the control group on the fifth day of infection (p < 0.01).…”

Section: Lhqw Attenuates Lung Index and Viral Loadmentioning

confidence: 95%

Identification of a Pharmacological Biomarker for the Bioassay-Based Quality Control of a Thirteen-Component TCM Formula (Lianhua Qingwen) Used in Treating Influenza A Virus (H1N1) Infection

Gao¹,

Niu

Wei

et al. 2020

Front. Pharmacol.

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As chemical analysis for quality control (QC) of traditional Chinese medicine (TCM) formula is difficult to guarantee the effectiveness, a bioassay method that combines QC with evaluation of therapeutic effects has been developed to assess the TCM quality. Here, we chose a thirteen-component TCM formula, Lianhua Qingwen capsule (LHQW), as a representative sample, to explore the pivotal biomarkers for a bioassay and to investigate close association between QC and pharmacological actions. Initially, our results showed that chemical fingerprinting could not effectively distinguish batches of LHQW. Pharmacological experiments indicated that LHQW could treat influenza A virus (H1N1) infection in the H1N1 mouse model, as claimed in clinical trials, by improving pathologic alterations and bodyweight loss, and decreasing virus replication, lung lesions and inflammation. Furthermore, by using serum metabolomics analysis, we identified two important metabolites, prostaglandin F 2a and arachidonic acid, and their metabolic pathway, arachidonic acid metabolism, as vital indicators of LHQW in treatment of influenza. Subsequently, macrophages transcriptomics highlighted the prominent role of cyclooxygenase-2 (COX-2) as the major rate-limiting enzyme in the arachidonic acid metabolism pathway. Finally, COX-2 was validated by in vivo gene expression and in vitro enzymatic activity with 43 batches of LHQW as a viable pharmacological biomarker for the

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Section: Lhqw Attenuates Lung Index and Viral Loadmentioning

confidence: 95%

Identification of a Pharmacological Biomarker for the Bioassay-Based Quality Control of a Thirteen-Component TCM Formula (Lianhua Qingwen) Used in Treating Influenza A Virus (H1N1) Infection

Gao¹,

Niu

Wei

et al. 2020

Front. Pharmacol.

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“…In einem Virus-Pneumonie-Mausmodell fand man außerdem, dass Ma huang tang oral für 7 Tage nach H1N1-Influenza-Infektion verabreicht, IL-2, IFN-γ sowie CD4 + -T-Zellen aktivierte und die CD4 + /CD8 + -Ratio verbesserte, jedoch IL-4, IL-5, TNF-α und die Expression von TLR4 und MyD88 hemmte (Wei et al 2018 Eine Wiener Arbeitsgruppe um den bekannten Allergieforscher Rudolf Valenta hat soeben ein Patent angemeldet, das als Impfung gegen Rhinoviren getestet wird. Mit der Zulassung ist allerdings erst in ca.…”

Section: Daraufhin Wurden Mäuse Mit Erdnussallergie Mit Fahf-2 Siebenunclassified

TCM

Stockert¹

2020

Allergieprävention

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“…erefore, many natural products that have traditionally been used for medicinal purposes can also serve as AIs with reduced side effects [48,49]. MHT has traditionally been used in the treatment of diseases such as influenza-like illnesses, sweating, asthma, fever, and female-related disorders [27,50,51]. However, the detailed mechanism of MHT in female-related disorders has not yet been clarified.…”

Section: Namementioning

confidence: 99%

Assessment of the Aromatase Inhibitory Activity of Ma-Huang-Tang (MHT) and Its Active Compounds

Jung

Hwang

Pyun

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Aromatase, a cytochrome P450 enzyme that converts androgens into estrogens, is an important drug target for hormone-dependent diseases. The purpose of this study was to elucidate the aromatase inhibitory effects of Ma-Huang-Tang (MHT), a traditional Korean herbal medicine prescription, and to identify its active ingredients. In this study, the inhibitory effect of MHT on aromatase activity was observed using dibenzylfluorescein (DBF) and KGN cells, and the dose-dependent effect of MHT was verified (IC50 values of 251 μg/mL and 246 μg/mL as determined by the two methods, respectively). Furthermore, among the six herbal medicines that constitute MHT, Ephedrae Herba, Cinnamomi Ramulus, and Glycyrrhizae Radix et Rhizoma showed the most potent inhibition of aromatase activity. Furthermore, upon identification of the active MHT compounds, three markers from Glycyrrhizae Radix et Rhizoma, liquiritin (5), liquiritin apioside (6), and liquiritigenin (7), were verified (IC50 values of 530 μM, 508 μM, and 1.611 mM and 499 μM, 522 μM, and 1.41 mM as determined by the two methods, respectively). In addition, their contents were confirmed to be 15.58, 19.80, and 2.22 mg/g, respectively, by HPLC/DAD analysis. These results indicate that the aromatase inhibitory effect of MHT results from the synergistic action of its active components and that MHT has potential as a preventive agent against aromatase activity.

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Antiviral effects of Ma Huang Tang against H1N1 influenza virus infection in vitro and in an ICR pneumonia mouse model

Cited by 19 publications

References 30 publications

Identification of a Pharmacological Biomarker for the Bioassay-Based Quality Control of a Thirteen-Component TCM Formula (Lianhua Qingwen) Used in Treating Influenza A Virus (H1N1) Infection

Identification of a Pharmacological Biomarker for the Bioassay-Based Quality Control of a Thirteen-Component TCM Formula (Lianhua Qingwen) Used in Treating Influenza A Virus (H1N1) Infection

TCM

Assessment of the Aromatase Inhibitory Activity of Ma-Huang-Tang (MHT) and Its Active Compounds

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