Antiviral effects of Yinhuapinggan granule against influenza virus infection in the ICR mice model

Peng, Xue-qian; Zhou, Huifen; Zhang, Yuyan; Yang, Jiehong; Wan, Haitong

doi:10.1007/s11418-015-0939-z

Cited by 22 publications

(22 citation statements)

References 47 publications

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“…Biomed Res 2018 Special Issue S424 Special Section:Medical Diagnosis and Study of Biomedical Imaging Systems and Applications based on Ma Huang Tang, has antiviral effects in IFV-infected mice, which is associated with the inhibition of the TLR4-MyD88-TRAF6 signal pathway. The results cited thus far indicate that TCM has a very well antiviral effect [20]. These findings suggest that YQP serum intervention groups decreased the expression of TLR4/MyD88/TRAF-6 and TLR3/ TRAM/TRIF pathway, especially in 3min groups and 6 min High-dose group.…”

Section: Discussionmentioning

confidence: 70%

The optimal decoction time of Yinqiao Powder on inhibit influenza A virus FM1 in mice

Chang¹,

Tan²,

Liu³

et al. 2018

biomedicalresearch

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Background: Yinqiao Powder (YQP) is administered in Traditional Chinese Medicine (TCM) for febrile infectious diseases and has been demonstrated to fight the prevalence of influenza. Objective: The present study aimed to identify the optimal decoction time of YQP on inhibit influenza A virus FM1 and investigate the molecular mechanisms of different decocting time YQP. Methods: The male BALB/c mice were made into pneumonia model of influenza A virus FM1. They were randomly assigned to twelve groups: blank control group, model control group, tamiflu (27.5 mg/Kg) group, 3 minutes YQP (High-dose group 30 g/Kg•d, Middle-dose group 15 g/Kg•d, and Low-dose group 7.5 g/Kg•d) groups, 6 minutes YQP (High-dose group 30 g/Kg•d, Middle-dose group 15 g/Kg•d, and Low-dose group 7.5 g/Kg•d) groups, and 12 minutes YQP (High-dose group 30 g/Kg•d, Middle-dose group 15 g/Kg•d, and Low-dose group 7.5 g/Kg•d) groups. All groups were administrated for 7 days. The mice were killed after the third and last days. Viral load of lung tissues were observed by Real-time PCR. Pulmonary index and inhibition rate were calculated by formula. Furthermore, the mice macrophage Ana-1 was infected by FM1, treated with different decocting time YQP drug-containing serum for 24 h. TLR3/4, MyD88, TRAF-6, TRAM and TRIF mRNA were detected by Real-time PCR. TLR3/4 proteins were observed by western blot. Results: All YQP intervention groups induced a reduction in the viral load and pulmonary index on the third and last days of infection, especially in 3 minutes YQP H/M/L groups and 6 minutes High-dose group. Meanwhile, YQP intervention groups led to significant decrease in TLR3/4, MyD88, TRAF-6, TRAM and TRIF mRNA levels and TLR3/4 proteins, in particular with 3 and 6 minutes YQP groups. Conclusion: YQP could protect the lung tissue and alleviate the inflammation caused by influenza A virus FM1. The optimal decoction time of YQP was 3to 6minutes on inhibit influenza A virus FM1 in mice.

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Section: Discussionmentioning

confidence: 70%

The optimal decoction time of Yinqiao Powder on inhibit influenza A virus FM1 in mice

Chang¹,

Tan²,

Liu³

et al. 2018

biomedicalresearch

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“…Our previous studies have demonstrated that YHPG has obvious antiviral, anti-inflammatory and antitussive effects in mice and rats [17][18][19][20]. The HPLC analysis clearly revealed the presence of the major constituents of YHPG (Fig.…”

Section: Discussionmentioning

confidence: 96%

Protective effects of Yinhuapinggan granule on mice with influenza viral pneumonia

Peng

Zhou

et al. 2016

International Immunopharmacology

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Yinhuapinggan granule (YHPG), a Chinese medicine granule on the basis of Ma-Huang-Tang (Ephedra Decoction) and the clinical experience of Professor Wan Haitong, has been shown to inhibit the growth of influenza virus in vitro. The aim of this study was to investigate the protective effects of YHPG on mice with influenza viral pneumonia and its effects on regulating related inflammatory cytokines in influenza virus A-infected mice. ICR mice were inoculated intranasally with 15 LD50 viral dose of influenza virus A/PR/8/34 (H1N1) and treatments with YHPG (7.5, 15 and 30g/kg) were orally administrated daily for 5 consecutive days after challenge, respectively. The results showed that mortality rate, lung index, lung histopathological changes, IL-6 and TNF-α in serum were significantly attenuated in the treatment of YHPG (15 and 30g/kg) than those in the IFV control group, while the levels of IL-2 was significantly enhanced. Moreover, the RT-PCR results revealed that YHPG (15 and 30g/kg) significantly depressed the expressions of IL-1β, IFN-γ and TNF-α mRNA in lung tissues. Furthermore, the immunohistochemical staining results also revealed that the expression of NF-κB p65 proteins was downregulated when treated with YHPG (15 and 30g/kg). These results showed YHPG has protective effects on IFV-infected mice, due to its ability of alleviation of lung damage, regulation of the cytokine production via inhibiting the NF-κB p65 activation, attenuation of systemic and pulmonary inflammatory responses.

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“…Previous researches have shown that YHPG has distinct antitussive, antiinflammatory and antibacterial effects (He et al 2014;Wan et al 2016). Further, antiviral studies has demonstrated that YHPG has significant effects on ameliorating influenza virus-induced inflammation, inhibiting TLRs/MyD88 mediated NF-jB signalling pathway, and inhibiting influenza virus replication (Peng et al 2016a(Peng et al , 2016b. However, the antiviral effects and its possible mechanism of YHPG in the apoptosis signalling pathway are still obscure.…”

Section: Discussionmentioning

confidence: 99%

“…According to the traditional dose ratio of 4 (Radix Puerariae Lobatae): 4 (Flos Lonicerae Japonicae): 4 (Polygoni Cuspidati Rhizoma): 2 (Ephedrae Herba): 2 (Armeniacae Semen Amarum): 1 (Glycyrrhizae Radix) ( Supplementary Table 1), YHPG granules were prepared by water-extraction and ethanol-precipitation, and the quality control of YHPG was also performed by HPLC, as reported in our previous research. HPLC analysis was carried out at 210 nm for the determination of eight active components in YHPG, containing L-ephedrine (0.73 mg/g), D-pseudoephedrine (0.407 mg/g), chlorogenic acid (1.92 mg/g), amygdalin (6.37 mg/g), puerarin (4.35 mg/g), polydatin (1.79 mg/g), glycyrrhizic acid (0.692 mg/g) and emodin (0.329 mg/g), respectively (Supplementary Figure 1) (Peng et al 2016a(Peng et al , 2016b.…”

Section: Materials and Reagentsmentioning

confidence: 99%

“…In previous research, YHPG has been shown to inhibit the reproduction of H1N1 influenza virus in embryonic eggs as a mixture of six herbs in different proportions (Peng et al 2005). In addition, YHPG has obvious protective effects on IFV-infected mice associated with alleviation of lung injury, regulation of cytokine production via NF-jB p65 activation and inhibition of TLR4-MyD88-TRAF signalling pathway in vivo (Peng et al 2016a(Peng et al , 2016b. In vitro, YHPG also has certain antiviral effects in IFV-infected RAW264.7 cells, which might be associated with regulation of inflammatory cytokines production, inhibition of TLR7-MyD88-IRF7 signalling pathway, activation of NF-jB p65, and modulation of the protein expressions of key effectors in the Type I IFN and PRRs signalling pathways (Du et al 2017(Du et al , 2018.…”

Section: Introductionmentioning

confidence: 99%

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Preliminary study of Yinhuapinggan granule against H1N1 influenza virus infection in mice through inhibition of apoptosis

Zhou

Yang

et al. 2020

Pharmaceutical Biology

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Context: Yinhuapinggan granule (YHPG) is frequently used for treating fever, cough, and viral pneumonia in traditional Chinese medicine. Objective: This study investigated the antiviral effects of YHPG in H1N1 influenza virus (IFV)-infected mice and its possible mechanism. Materials and methods: ICR mice were intranasally infected with 10 LD 50 viral dose of IFV and then oral administration of YHPG (6, 12, and 18 g/kg) or oseltamivir (positive control) once a day for 2 or 4 consecutive days, six mice in each group. The lung, spleen and thymus indexes of IFV-infected mice, the expression of viral loads and pathological changes in lung tissues were performed to evaluate the antiviral effects of YHPG. Real-time PCR, immunohistochemistry and western blot assays were used to determine the expression of Bax, Bcl-2 and caspase-3. Results: LD 50 in mice was 10 À3.5 /0.02 mL. YHPG (6, 12, and 18 g/kg) dose-dependently decreased the lung index and viral load; the inhibition ratio of lung index was 5.31, 18.22, and 34.06%, respectively. Further detection revealed that YHPG (12 and 18 g/kg) significantly attenuated lung pathological changes, and increased the spleen and thymus indexes. Moreover, YHPG significantly down-regulated the mRNA and protein expression of Bax and caspase-3 in lung tissues of mice infected with IFV, and up-regulated the expression of Bcl-2. Conclusions: YHPG has significant antiviral effects in IFV-infected mice, partially by inhibiting influenza virus replication and regulating the occurrence of apoptosis induced by influenza virus infection, suggesting that YHPG may be a promising antiviral agent with potential clinical application prospects.

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Antiviral effects of Yinhuapinggan granule against influenza virus infection in the ICR mice model

Cited by 22 publications

References 47 publications

The optimal decoction time of Yinqiao Powder on inhibit influenza A virus FM1 in mice

The optimal decoction time of Yinqiao Powder on inhibit influenza A virus FM1 in mice

Protective effects of Yinhuapinggan granule on mice with influenza viral pneumonia

Preliminary study of Yinhuapinggan granule against H1N1 influenza virus infection in mice through inhibition of apoptosis

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