Anwulignan Ameliorates the Intestinal Ischemia/Reperfusion

Lin, Hongyuan; Zhang, Xinyun; Wang, Dan; Liu, Jiawei; Yuan, Linhong; Liu, Jiale; Wang, Chunmei; Sun, Jia; Chen, Jianguang; He, Li; Shu, Jiwu

doi:10.1124/jpet.121.000587

Cited by 4 publications

(2 citation statements)

References 53 publications

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“…Forty eight mice were randomly divided into four groups, 10 in each group (Abd El-Ghffar et al, 2018;Wang et al, 2020) L et al, 2018;Badr et al, 2019;Li, Hu et al, 2018). AN alone had no significant effect on healthy mice (Zhang et al, 2020;Lin et al, 2021), and the dose for this study was determined based on the results reported by us previously (Gao et al, 2018;Liu et al, 2021). Mice in the IND, AN, and OME groups were intragastrically given the corresponding dose of the different agents as previously described once daily for 14 days, whereas those in the CON group were administered an equal volume of the solvent (sodium carboxymethyl cellulose) in the same way.…”

Section: Methodsmentioning

confidence: 99%

Protective Effect of Anwulignan on Gastric Injury Induced by Indomethacin in Mice

Liu

Fang

Zhang

et al. 2022

J Pharmacol Exp Ther

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Anwulignan (AN) is a monomer lignan from Schisandra sphenanthera Rehd. et Wits (Schisandra sphenanthera fructus, Schisandra sphenanthera). The protective effect of AN against the indomethacin (IND)-induced gastric injury to mice and the related mechanism of action was investigated in this study. The effect of AN was mainly assessed by observing the gastric tissue morphology, gastric ulcer index (GUI), ulcer inhibition rate (UIR), gastric juice volume (GJV) and pH value. Chemical colorimetry, immunofluorescence, ELISA, and Western blot were used to detect related factors in the gastric tissue. The results showed that AN reduced the GUI, increased the UIR, inhibited the GJV, and increased the gastric pH value. AN significantly increased cyclooxygenase-1, cyclooxygenase-2, and prostaglandin E2 expression levels in the gastric tissue, activated nuclear factor (erythroid-derived 2)-like 2 (Nrf2), increased heme oxygenase-1 expression, enhanced the activity of superoxide dismutase and glutathione peroxidase, and decreased the malondialdehyde content. AN reduced the phosphorylation of nuclear factor-k gene binding (NF-kB) p65 and its nuclear translocation, the key protein of NF-kB signaling pathway in the gastric tissue, and the content of the pathway downstream signaling molecules, including interleukin-6, interleukin-1b, and tumor necrosis factor-a, to play an anti-inflammatory role. AN inhibited the downstream signals B-cell lymphoma 2associated x protein and cleaved caspase-3 in gastric tissue, and activated B-cell lymphoma 2, to play an antiapoptotic role, which were further verified by Hoechst staining. Therefore, AN has a significant protection against the gastric injury induced by IND in mice, and the mechanism may be concerned in its activation of Nrf2, inhibition of NF-kB signaling pathway, and antiapoptotic effect. SIGNIFICANCE STATEMENTAnwulignan (AN) significantly reduced the indomethacininduced gastric injury in mice, and its antioxidation, antiinflammation, and antiapoptosis were considered to be involve in the effect, suggesting that AN should be a potential drug or food supplement for gastric injury induced by indomethacin.

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Section: Methodsmentioning

confidence: 99%

Protective Effect of Anwulignan on Gastric Injury Induced by Indomethacin in Mice

Liu

Fang

Zhang

et al. 2022

J Pharmacol Exp Ther

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“…Anwulignan (AN) is a highly representative lignan active monomer compound extracted from Schisandra chinensis, a traditional Chinese medicine 19 . Our previous studies have indicated that AN has strong antioxidant and anti-in ammatory activities in fatigue animal models 20 and D-galactose-induced aging mouse models 21 been no relevant report on the protective effect of AN against KOA so far.…”

Section: Introductionmentioning

confidence: 99%

Therapeutic Effect of Anwulignan on Knee Osteoarthritis and Its Mechanism through Cell Pyroptosis Pathway in Rats

Niu,

Jiang,

Zhao

et al. 2023

Preprint

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Anwulignan (AN) is a monomer compound in the lignans of Schisandra chinensis. In this study, the effect of AN on knee osteoarthritis (KOA) in male SD rats was observed and its potential mechanisms were explored. The results showed that AN could significantly increase the number of standing times of rats within the same time, reduce the degree of knee joint swelling in rats, and alleviate the damage of cartilage tissue. AN could increase the activity of superoxide dismutase (SOD), and decrease the contents of malondialdehyde (MDA) and reactive oxygen species (ROS) in the synovial tissue of rats, and reduce the levels of inflammatory factors such as tumor necrosis factor-α (TNF-α), interlukin (IL)-6, IL-1β and IL-18, prostaglandin E2 (PGE2) and nitric oxide (NO) in the serum and synovial tissue of rats. AN could play an antioxidant role by regulating the nuclear factor erythroid E2-related factor 2 (Nrf2) signaling pathway-related proteins, and play anti-inflammatory and anti-pyroptosis roles by regulating pyroptosis pathway-related proteins, including the nuclear factor-k-gene binding (NF-κB) signaling pathway-related proteins and NOD-like receptor family pyrin domain containing 3 (NLRP3). AN could significantly increase the expression level of CollagenII protein in KOA rats and decrease the expression levels of matrix metalloproteinase1 (MMP1) and matrix metalloproteinase13 (MMP13) proteins. The results of immunofluorescence analysis further confirmed the protective effect of AN against KOA in rats, which may be related to the regulation of Nrf2/HO-1 signaling pathway to play an antioxidant role and the regulation of TLR4/NLRP3/Caspase-1 signaling pathway-mediated anti-pyroptosis effect.

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Natural Products Improve Organ Microcirculation Dysfunction Following Ischemia/Reperfusion- and Lipopolysaccharide-Induced Disturbances: Mechanistic and Therapeutic Views

Han,

Li,

Sun

et al. 2024

Engineering

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Anwulignan Ameliorates the Intestinal Ischemia/Reperfusion

Cited by 4 publications

References 53 publications

Protective Effect of Anwulignan on Gastric Injury Induced by Indomethacin in Mice

Protective Effect of Anwulignan on Gastric Injury Induced by Indomethacin in Mice

Therapeutic Effect of Anwulignan on Knee Osteoarthritis and Its Mechanism through Cell Pyroptosis Pathway in Rats

Natural Products Improve Organ Microcirculation Dysfunction Following Ischemia/Reperfusion- and Lipopolysaccharide-Induced Disturbances: Mechanistic and Therapeutic Views

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