Anxiety- and Depressive-Like Behaviors are Associated with Altered Hippocampal Energy and Inflammatory Status in a Mouse Model of Crohn’s Disease

Haj‐Mirzaian, Arya; Amiri, Shayan; Amini‐Khoei, Hossein; Hosseini, Mir-Jamal; Haj-Mirzaian, Arvin; Momeny, Majid; Rahimi-Balaei, Maryam; Dehpour, Ahmad Reza

doi:10.1016/j.neuroscience.2017.10.023

Cited by 76 publications

(68 citation statements)

References 112 publications

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“…Cluster analysis has also been utilized for gene expression analysis and associated disease outcomes [ 35 ] and recently to classify plantar pressure distribution, which is critical for the prevention and/or treatment of the diabetic foot [ 36 ]. The DSS-induced acute mouse model of colitis represents a multidimensional model with various inherent features of severity such as anxiety/depression and pain [ 37 , 38 ]. Therefore, we considered data derived from this model as an optimal ‘training data set.’ Consequently, VWR and body weight as objective, observer independent data were used to develop a cluster model.…”

Section: Discussionmentioning

confidence: 99%

Running in the wheel: Defining individual severity levels in mice

et al. 2018

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The fine-scale grading of the severity experienced by animals used in research constitutes a key element of the 3Rs (replace, reduce, and refine) principles and a legal requirement in the European Union Directive 2010/63/EU. Particularly, the exact assessment of all signs of pain, suffering, and distress experienced by laboratory animals represents a prerequisite to develop refinement strategies. However, minimal and noninvasive methods for an evidence-based severity assessment are scarce. Therefore, we investigated whether voluntary wheel running (VWR) provides an observer-independent behaviour-centred approach to grade severity experienced by C57BL/6J mice undergoing various treatments. In a mouse model of chemically induced acute colitis, VWR behaviour was directly related to colitis severity, whereas clinical scoring did not sensitively reflect severity but rather indicated marginal signs of compromised welfare. Unsupervised k-means algorithm–based cluster analysis of body weight and VWR data enabled the discrimination of cluster borders and distinct levels of severity. The validity of the cluster analysis was affirmed in a mouse model of acute restraint stress. This method was also applicable to uncover and grade the impact of serial blood sampling on the animal’s welfare, underlined by increased histological scores in the colitis model. To reflect the entirety of severity in a multidimensional model, the presented approach may have to be calibrated and validated in other animal models requiring the integration of further parameters. In this experimental set up, however, the automated assessment of an emotional/motivational driven behaviour and subsequent integration of the data into a mathematical model enabled unbiased individual severity grading in laboratory mice, thereby providing an essential contribution to the 3Rs principles.

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Section: Discussionmentioning

confidence: 99%

Running in the wheel: Defining individual severity levels in mice

et al. 2018

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“…At the end to the test, the apparatus was cleaned up with a 5% ethanol solution. Anxiety‐like behavior was assessed through two parameters: percentage of time spent on the open arms (OAT%) and the percentage of entries into the open arms (OAE%; any increase in the OAT% and/or OAE% was considered as anxiolytic‐like behavior) …”

Section: Methodsmentioning

confidence: 99%

Pro‐neurocognitive and anti‐sarcopenic benefits of one‐year metformin therapy in ovariectomized aged mice

Zakeri

Fatemi

Kaeidi

et al. 2019

Clin Exp Pharma Physio

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Health promotion and healthy nutrition significantly increased life expectancy around the world. Aging is associated with an increase in age-related diseases. The use of metformin (Met) as an anti-aging drug has recently been proposed based on its widespread use in clinical practice. Reports have shown that Met acts as an antiaging agent. In this study, the effects of long-term, 1 year, Met administration on aging-related behaviors and longevity in ovariectomized mice was studied. Met (1 and 10 mg/kg, daily) was administered orally in ovariectomized mice. The anxiety-like behavior, working memory, and physical strength were measured through elevated plus maze, Y-maze, vertical grid holding, and the obligatory swimming capacity tests.Brains were harvested to measure brain-derived neurotrophic factor (BDNF) level.Also, the Kaplan-Meier survival curves were used to show differences and similarities in survival patterns. Met (10 mg/kg) decreased anxiety-like behaviors as well as increased muscle strength and working memory in the ovariectomized mice. Moreover, Met increased the physical strength and longevity as well as the level of BDNF in the ovariectomized mice. Our results indicate that Met administration can be an effective strategy for having a healthy aging in the absence of female gonadal hormones and reverses deleterious effects of ovariectomy-induced aging possibly through BDNF. K E Y W O R D Saging, brain-derived neurotrophic factor, metformin, ovariectomy

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“…It is rapidly synthesized from l ‐arginine by nitric oxide synthase (NOS), an enzyme group consisting of three subtypes including inducible (iNOS), endothelia NOS (eNOS) and neuronal NOS (nNOS) (Haj‐Mirzaian et al, ; Zhang et al, ). Behavioral abnormalities associated with high levels of NO have been widely reported in literature (Haj‐Mirzaian et al, , , ; Harkin, Connor, Burns, & Kelly, ; Lorigooini, Salimi, Soltani, & Amini‐Khoei, ; Ulak et al, ; Volke, Wegener, Bourin, & Vasar, ). Previous studies have established that NO is involved in several disease conditions characterized of oxidative stress and inflammation, and thus contributes significantly to the associated behavioral impairments (Chatterjee et al, 2012; Haj‐Mirzaian et al, , ).…”

Section: Introductionmentioning

confidence: 91%

“…Behavioral abnormalities associated with high levels of NO have been widely reported in literature (Haj‐Mirzaian et al, , , ; Harkin, Connor, Burns, & Kelly, ; Lorigooini, Salimi, Soltani, & Amini‐Khoei, ; Ulak et al, ; Volke, Wegener, Bourin, & Vasar, ). Previous studies have established that NO is involved in several disease conditions characterized of oxidative stress and inflammation, and thus contributes significantly to the associated behavioral impairments (Chatterjee et al, 2012; Haj‐Mirzaian et al, , ). l ‐Arginine, the precursor substrate responsible for the synthesis of NO, has been reported to produce depressive‐like behaviors, which were abolished by nitric oxide synthase inhibitors (Ergun & Ergun, ; Haj‐Mirzaian et al, ; Lorigooini et al, ).…”

Section: Introductionmentioning

confidence: 91%

Involvement of l‐arginine‐nitric oxide pathway in the antidepressant and memory promoting effects of morin in mice

Ben‐Azu

Aderibigbe

Ajayi

et al. 2019

Drug Development Research

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l‐Arginine‐nitric oxide pathway has been reported to be involved in the mediation of the psychopharmacological effects of many psychotropic drugs. Previous studies have shown that morin, a psychotropic compound isolated from mulberry leaf produces functional psychopharmacological effects indicative of antidepressant, antipsychotic, anxiolytic and nootropic properties. However, the role of l‐arginine‐nitric oxide pathway in the psychotropic effects of morin has not been fully investigated, hence, the need for this study. Male Swiss mice were pretreated individually or in combination with nitric oxide precursor [l‐arginine (750 mg/kg, i.p.)], competitive nonselective nitric oxide synthase (NOS) inhibitor [N(G)‐nitro‐l‐arginine methyl ester (l‐NAME, i.p) (50 mg/kg)] or selective neuronal NOS inhibitor [methylene blue (3.75 mg/kg, i.p)] prior to morin (100 mg/kg, i.p.) or saline (10 mL/kg, i.p.) treatment. Psychopharmacological activities were then evaluated 30 min later using open field, Y‐maze, and forced swim tests. l‐Arginine significantly reversed the effects of morin on locomotion, memory and depression in mice. The reduced motor activity and enhanced memory function produced by morin were significantly attenuated by methylene blue but augmented the antimobility activity of morin in the FST. Moreover, l‐NAME potentiated the psychopharmacological effects of morin in the open field and forced swim tests but reduced its memory promoting effect. Meanwhile, morin supplementation reversed the effects of l‐arginine on l‐NAME‐treated mice in all behavioral models. The results of this study suggest that l‐arginine‐nitric oxide pathway might play a role in the modulation of the antidepressant and memory promoting effects of morin in mice.

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Anxiety- and Depressive-Like Behaviors are Associated with Altered Hippocampal Energy and Inflammatory Status in a Mouse Model of Crohn’s Disease

Cited by 76 publications

References 112 publications

Running in the wheel: Defining individual severity levels in mice

Running in the wheel: Defining individual severity levels in mice

Pro‐neurocognitive and anti‐sarcopenic benefits of one‐year metformin therapy in ovariectomized aged mice

Involvement of l‐arginine‐nitric oxide pathway in the antidepressant and memory promoting effects of morin in mice

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