Anxiolytic activity of Nymphaea alba Linn. in mice as experimental models of anxiety

Abstract

Objective:The aim of the present work was to evaluate the anxiolytic effect of an ethanolic extract of Nymphaea alba Linn. in mice.Materials and Methods:The elevated plus maze test (EPMT), light and dark test (L and DT) and open field test (OFT) were used to assess the anxiolytic activity of the ethanolic extract of N. alba Linn. in mice. In addition, aggressive behavior and motor coordination was also assessed by foot shock induced aggression test (FSIAT) and rota rod test (RRT). Diazepam 1 mg/kg served as a standard anxiolytic drug, administered orally.Results:The ethanolic extract of N. alba (100 and 200 mg/kg, p.o.) significantly increased the percentage of time spent and number of entries in open arm in EPMT. In L and DT, the extract produced significant increase in time spent, number of crossing and decrease in the duration of immobility in light box. In OFT, the extract showed significant increase in number of rearings, assisted rearings and number of square crossed, all of which are demonstrations of exploratory behavior. In FSIAT, N. alba extract attenuated aggressive behavior related to anxiolytic activity, such as number of vocalization, leaps, rearing, biting/attacks and facing each other in paired mice. Furthermore, the extract produced skeletal muscle relaxant effect assessed by RRT.Conclusion:The results of the present study suggest that an ethanolic extract of N. alba may possess anxiolytic activity and provide a scientific evidence for its traditional claim.

Pharmacological evaluation of anxiolytic property of aqueous root extract of Cymbopogon citratus in mice

“…This portrays a manifestation of anxiety. [34] The open field model examines related psychomotor performance, normal aversion and behavioral aspect of [35] due to thoughts of imminent threat. The locomotive activity of the mice significantly (P < 0.05) increased at extract doses of 200 mg/kg and 600 mg/kg across the square of the open field, higher at these doses of the extract compared to diazepam and the normal saline group.…”

Acute and Sub-acute Toxicity Profile of Aqueous Leaf Extract of <i>Nymphaea lotus</i> Linn (Nymphaeaceae) in Wistar Rats

“…Many biological activities, including anticancer and antiviral, has been attributed to gallic and ellagic acid which are widely present in N. lotus [8]. Our previous study also showed that the aqueous extract of N. lotus is rich in phytochemicals such as proanthocyanidins, phenolics, tannins, flavonoids and saponins [9].…”

Phytopharmaceutical Potentials of Prosopis Laevigata, Symplocos Cochinchinensis and Nymphaea Alba: A Review

“…Both In situ and In vivo studies have shown that the plant exudates contain polysaccharides and proteins which make it efficient for gummosis [49]. This property of P. laevigata can be explored further to be used in confectionary, sauces, jellies, spray drying of juices, bakery products, adhesives, fixatives, micro encapsulations, wood furniture, gels, cosmetics, and various other industrial uses.…”