Aortic Dissection in Marfan Syndrome

Braverman, Alan C.

doi:10.1016/j.jacc.2023.08.054

Cited by 1 publication

(1 citation statement)

References 17 publications

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“…Aortic dissection (AD) is a highly prevalent and fatal acute cardiovascular disease, making early diagnosis of AD crucial. 35,36 Emerging evidence indicates that microRNAs play a role in the development and progression of AD by modulating the expression of specific target genes and influencing the activity of associated proteins. This experiment utilized miRNA-155 (miR-155) associated with AD as the detection model.…”

Section: ■ Introductionmentioning

confidence: 99%

High-Sensitivity Homogeneous Detection of miRNA-155 Governed by DNA Walker-Regulated Surface DNA Density of Magnetic Electrochemiluminescence Nanoparticles

Lin,

Luo,

Luo

et al. 2024

Anal. Chem.

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Homogeneous electrochemiluminescence (ECL) has gained attention for its simplicity and stability. However, false positives due to solution background interference pose a challenge. To address this, magnetic ECL nanoparticles (Fe3O4@Ru@SiO2 NPs) were synthesized, offering easy modification, magnetic separation, and stable luminescence. These were utilized in an ECL sensor for miRNA-155 (miR-155) detection, with locked DNAzyme and substrate chain (mDNA) modified on their surface. The poor conductivity of long-chain DNA significantly impacts the conductivity and electron transfer capability of Fe3O4@Ru@SiO2 NPs, resulting in weaker ECL signals. Upon target presence, unlocked DNAzyme catalyzes mDNA cleavage, leading to shortened DNA chains and reduced density. In contrast, the presence of short-chain DNA has minimal impact on the conductivity and electron transfer capability of Fe3O4@Ru@SiO2 NPs. Simultaneously, the material surface’s electronegativity decreases, weakening the electrostatic repulsion with the negatively charged electrode, resulting in the system detecting stronger ECL signals. This sensor enables homogeneous ECL detection while mitigating solution background interference through magnetic separation. Within a range of 100 fM to 10 nM, the sensor exhibits a linear relationship between ECL intensity and target concentration, with a 26.91 fM detection limit. It demonstrates high accuracy in clinical sample detection, holding significant potential for clinical diagnostics. Future integration with innovative detection strategies may further enhance sensitivity and specificity in biosensing applications.

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Section: ■ Introductionmentioning

confidence: 99%