AP-1 overexpression impairs corticosteroid inhibition of collagen production by fibroblasts isolated from asthmatic subjects

Jacques, Éric; Semlali, Abdelhabib; Boulet, Louis Philippe; Chakir, Jamila

doi:10.1152/ajplung.00360.2009

Cited by 20 publications

(15 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Even if LABA/ SABA strongly inhibited EBF proliferation, both drug groups did not surprisingly alter collagen deposition, indicating this effect does not involve β 2 -adrenoceptor stimulation, while other studies (Lamyel et al, 2011;Saltzman et al, 1982) showed rather decreased collagen synthesis by β 2 -agonists in permanent serum-starved lung fibroblasts. On the other hand, the dexamethasone-induced reduction in serum-induced collagen synthesis was in agreement with data found in human bronchial fibroblasts (Jacques et al, 2010).…”

Section: Discussionsupporting

confidence: 91%

Concomitant inhibition of primary equine bronchial fibroblast proliferation and differentiation by selective β2-adrenoceptor agonists and dexamethasone

Franke

Abraham

2014

European Journal of Pharmacology

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 91%

Concomitant inhibition of primary equine bronchial fibroblast proliferation and differentiation by selective β2-adrenoceptor agonists and dexamethasone

Franke

Abraham

2014

European Journal of Pharmacology

View full text Add to dashboard Cite

“…26,29–33 The transcription factor ATF4 is also regulated by β2-adrenoreceptor agonists and related to the promotion of AHR in asthma. 34 EGR1 has similarly been linked to the pathogenesis of allergic asthma, where it regulates gene transcription in many pro-inflammatory and allergic responses, such as immunoglobulin E (IgE) and TNF production, and also promotes AHR in allergic asthma.…”

Section: Discussionmentioning

confidence: 99%

Untargeted Proteomics and Systems-Based Mechanistic Investigation of Artesunate in Human Bronchial Epithelial Cells

Ravindra

Chang

et al. 2015

Chem. Res. Toxicol.

View full text Add to dashboard Cite

The anti-malarial drug artesunate is a semi-synthetic derivative of artemisinin, the principal active component of a medicinal plant Artemisia annua. It is hypothesized to attenuate allergic asthma via inhibition of multiple signaling pathways. We used a comprehensive approach to elucidate the mechanism of action of artesunate by designing a novel biotinylated dihydroartemisinin (BDHA) to identify cellular protein targets of this anti-inflammatory drug. By adopting an untargeted proteomics approach, we demonstrated that artesunate may exert its protective anti-inflammatory effects via direct interaction with multiple proteins, most importantly with a number of mitochondrial enzymes related to glucose and energy metabolism, along with mRNA and gene expression, ribosomal regulation, stress responses and structural proteins. In addition, the modulatory effects of artesunate on various cellular transcription factors were investigated using a transcription factor array, which revealed that artesunate can simultaneously modulate multiple nuclear transcription factors related to several major pro- and anti-inflammatory signalling cascades in human bronchial epithelial cells. Artesunate significantly enhanced nuclear levels of nuclear factor erythroid-2-related factor 2 (Nrf2), a key promoter of antioxidant mechanisms, which is inhibited by the Kelch-like ECH-associated protein 1 (Keap1). Our results demonstrate that, like other electrophilic Nrf2 regulators, artesunate activates this system via direct molecular interaction/modification of Keap1, freeing Nrf2 for transcriptional activity. Altogether, the molecular interactions and modulation of nuclear transcription factors provide invaluable insights into the broad pharmacological actions of artesunate in inflammatory lung diseases and related inflammatory disorders.

show abstract

“…Culture of primary bronchial fibroblasts exists for human [11,12], mice [13] and rats [14]. However, there is currently neither description of equine adult primary airway fibroblast cultures nor there are such cell lines for this species.…”

Section: Introductionmentioning

confidence: 99%

Comparative study of the effects of fetal bovine serum versus horse serum on growth and differentiation of primary equine bronchial fibroblasts

et al. 2014

View full text Add to dashboard Cite

BackgroundAirway fibroblasts have become a critical addition to all facets of structural lung tissue changes such as in human asthma and chronic obstructive pulmonary disease, but little is known about their role in the equine recurrent airway obstruction, a disease that resembles to the human asthma. Since the equine bronchial fibroblasts (EBF) have not been isolated and characterized yet, the use of defined medium was investigated.ResultsPrimary EBF were cultured on non-collagen coated flasks without serum or in the presence of fetal bovine serum (FBS) or horse serum (HS) or in serum depleted medium. EBF cultured in serum-free basal media and those serum deprived were not able to proliferate and even exhibited considerable cell death. In media containing FBS or HS, proliferation of the cells was reproducible between different primary cultures and cells demonstrated expression of vimentin. Large variations were found in the ability of FBS and HS to support growth and differentiation of EBF in monolayer culture. Indications of growth-promoting actions, increasing passage number as well as maintaining fibroblast morphology were found rather in FBS than in HS. EBF culturing in HS needed longer doubling and confluence time. The protein content of the cell pellets was higher in EBF cultured in medium containing HS than FBS. Alpha-smooth muscle actin seemed to be less expressed in EBF cultured in medium containing FBS than those in HS.ConclusionsIn sum, serum addition to basal EBF medium enhanced EBF differentiation into myofibroblasts, and these findings are useful to develop in vitro fibroblast culture models that mimic in vivo physiological processes and to study airway disease mechanisms and remodeling.

show abstract

AP-1 overexpression impairs corticosteroid inhibition of collagen production by fibroblasts isolated from asthmatic subjects

Abstract: Jacques E, Semlali A, Boulet LP, Chakir J. AP-1 overexpression impairs corticosteroid inhibition of collagen production by fibroblasts isolated from asthmatic subjects.

Cited by 20 publications

References 34 publications

Concomitant inhibition of primary equine bronchial fibroblast proliferation and differentiation by selective β2-adrenoceptor agonists and dexamethasone

Concomitant inhibition of primary equine bronchial fibroblast proliferation and differentiation by selective β2-adrenoceptor agonists and dexamethasone

Untargeted Proteomics and Systems-Based Mechanistic Investigation of Artesunate in Human Bronchial Epithelial Cells

Comparative study of the effects of fetal bovine serum versus horse serum on growth and differentiation of primary equine bronchial fibroblasts

Contact Info

Product

Resources

About