Apamer-based sensitive and label-free electrochemical detection of neutrophil gelatinase-associated lipocalin via recycling amplification cascades

Yang, Jianmei; Gan, Xiaoyan; Song, Xinmei; Yuan, Ruo; Xiang, Yun

doi:10.1016/j.aca.2022.340515

Cited by 7 publications

(3 citation statements)

References 33 publications

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“…blank. Although others have reported a similar performance using gold electrodes in a standard three-electrode cell or improved sensitivity using a combination of carbon electrodes and nanomaterials, as presented in Table 7, this work was the first in which such a detection was demonstrated using a commercially available gold SPE for the electrochemical immunosensor in bodily fluids [47,[58][59][60][61][62][63][64][65][66]. The logistic regression equation is given as 𝐼 = 𝐴 + 𝐵 × ln (𝑥), where A is 228 and B is 202, with an r 2 = 0.9822.…”

Section: Sensing Platform Evaluationmentioning

confidence: 70%

“…It shows a linear relationship between the steady-state current and the logarithmic values of the antigen concentration in the range from 3.5 ng/mL to 80 ng/mL with a theoretical detection limit (LOD) of 1.0 ng/mL, estimated as the mean blank level + 3 × standard deviation of the blank. Although others have reported a similar performance using gold electrodes in a standard three-electrode cell or improved sensitivity using a combination of carbon electrodes and nanomaterials, as presented in Table 7, this work was the first in which such a detection was demonstrated using a commercially available gold SPE for the electrochemical immunosensor in bodily fluids [47,[58][59][60][61][62][63][64][65][66].…”

Section: Sensing Platform Evaluationmentioning

confidence: 71%

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Amperometric Biosensor for Quantitative Measurement Using Sandwich Immunoassays

et al. 2023

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State-of-the-art clinical detection methods typically involve standard immunoassay methods, requiring specialized equipment and trained personnel. This impedes their use in the Point-of-Care (PoC) environment, where ease of operation, portability, and cost efficiency are prioritized. Small, robust electrochemical biosensors provide a means with which to analyze biomarkers in biological fluids in PoC environments. Optimized sensing surfaces, immobilization strategies, and efficient reporter systems are key to improving biosensor detection systems. The signal transduction and general performance of electrochemical sensors are determined by surface properties that link the sensing element to the biological sample. We analyzed the surface characteristics of screen-printed and thin-film electrodes using scanning electron microscopy and atomic force microscopy. An enzyme-linked immunosorbent assay (ELISA) was adapted for use in an electrochemical sensor. The robustness and reproducibility of the developed electrochemical immunosensor were investigated by detecting Neutrophil Gelatinase-Associated Lipocalin (NGAL) in urine. The sensor showed a detection limit of 1 ng/mL, a linear range of 3.5–80 ng/mL, and a CV% of 8%. The results demonstrate that the developed platform technology is suitable for immunoassay-based sensors on either screen-printed or thin-film gold electrodes.

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Section: Sensing Platform Evaluationmentioning

confidence: 70%

Section: Sensing Platform Evaluationmentioning

confidence: 71%

Amperometric Biosensor for Quantitative Measurement Using Sandwich Immunoassays

et al. 2023

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“…NGAL is mainly expressed and secreted by immune cells, liver cells or renal tubular cells, and it can capture and consume siderophore to play an antibacterial role ( 20 , 21 ). In addition to antibacterial, NGAL can also be used as a factor regulating cell growth and differentiation, mediating the biological activity of iron inside and outside cells ( 22 ).…”

Section: Discussionmentioning

confidence: 99%

Serum neutrophil gelatinase-associated lipocalin as a potential biomarker for cognitive decline in spinal cord injury

Zhang

Li²,

Xie³

et al. 2023

Front. Neurol.

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ObjectiveNeutrophil gelatinase-associated lipoprotein (NGAL), a protein encoded by the lipocalcin-2 (LCN2) gene, has been reported to be involved in multiple processes of innate immunity, but its relationship with spinal cord injury (SCI) remains unclear. This study set out to determine whether NGAL played a role in the development of cognitive impairment following SCI.MethodsAt the Neck-Shoulder and Lumbocrural Pain Hospital, a total of 100 SCI patients and 72 controls were enrolled in the study through recruitment. Through questionnaires, baseline data on the participants' age, gender, education level, lifestyle choices (drinking and smoking) and underlying illnesses (hypertension, diabetes, coronary heart disease, and hyperlipidemia) were gathered. The individuals' cognitive performance was evaluated using the Montreal Cognitive Scale (MoCA), and their serum NGAL levels were discovered using ELISA.ResultsThe investigation included 72 controls and 100 SCI patients. The baseline data did not differ substantially between the two groups, however the SCI group's serum NGAL level was higher than the control group's (p < 0.05), and this elevated level was adversely connected with the MoCA score (p < 0.05). According to the results of the ROC analysis, NGAL had a sensitivity of 58.24% and a specificity of 86.72% for predicting cognitive impairment following SCI.ConclusionsThe changes in serum NGAL level could serve as a biomarker for cognitive impairment in SCI patients, and this holds true even after taking in account several confounding variables.

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Microfluidic chip immunoassay based on rolling circle amplification and G-quadruplex/Thioflavin T for multiplex detection of CTX I

Jiang,

Lv,

et al. 2024

Microchim Acta

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Apamer-based sensitive and label-free electrochemical detection of neutrophil gelatinase-associated lipocalin via recycling amplification cascades

Cited by 7 publications

References 33 publications

Amperometric Biosensor for Quantitative Measurement Using Sandwich Immunoassays

Amperometric Biosensor for Quantitative Measurement Using Sandwich Immunoassays

Serum neutrophil gelatinase-associated lipocalin as a potential biomarker for cognitive decline in spinal cord injury

Microfluidic chip immunoassay based on rolling circle amplification and G-quadruplex/Thioflavin T for multiplex detection of CTX I

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