Apatite Deposition on Polyamide Films Containing Carboxyl Groups in Body Environment

Miyazaki, Toshiki; Akioka, Y.; Ohtsuki, Chikara; Tanihara, Masao; Nakao, Junko; Sakaguchi, Yoshimitsu; Konagaya, Shigeji

doi:10.4028/www.scientific.net/kem.218-220.133

Cited by 52 publications

(66 citation statements)

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“…The concentrations of each reagent in the solutions are summarized in Table 2. CaCl 2 concentrations were determined by referring to previous papers that describe apatite formation on CaCl 2-treated organic polymers 12,13) . Then the solution was adjusted to pH 7 by dropwise addition of 1 M NaOH.…”

Section: Methodsmentioning

confidence: 99%

“…PGA is reported to have excellent apatite-forming ability in SBF, since it contains abundant carboxyl groups, which are effective for heterogeneous apatite nucleation 12,13) . The problem of poor apatite formation on chemically synthesized collagen would be solved by immobilization of an organic polymer, with high apatite formation ability (such as PGA) onto the collagen.…”

Section: Introductionmentioning

confidence: 99%

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Biomineralization on chemically synthesized collagen containing immobilized poly-γ-glutamic acid

et al. 2013

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Chemically synthesized collagen with a triple helix structure similar to that of natural collagen is attractive as a safe biomaterial. Hybrids of chemically synthesized collagen and apatite are proposed for novel bone substitutes. However their apatite-forming ability in simulated body fluid is still quite low. We examined acceleration of apatite formation on collagen by immobilized poly-γ-glutamic acid (PGA), which has excellent apatite-forming ability. Apatite was formed within 3 days when collagen was treated with PGA solutions containing an appropriate amount of CaCl 2 . A mixture of apatite and calcite was formed at high CaCl 2 concentration. The present results indicate the possibility of preparing hybrid materials, with tailored mechanical and biological properties, based on chemically synthesized collagen.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biomineralization on chemically synthesized collagen containing immobilized poly-γ-glutamic acid

et al. 2013

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“…(-NH 3+ ) groups, this organic multilayered template is able to regulate biomineral formation and overcome the dispersion problem of CaP particles on PET fibers (17). In addition, the carboxylic groups interact with the calcium ions of CaP, which enables the multilayered template to effectively stimulate BM and induce the deposition of crystalline CaP (18)(19)(20). However, in contrast to other fast methods for biomimetic deposition of nanocrystalline apatite, the current technique requires approximately three days to deposit a uniform nanoscale CaP coating on the PET ligament, which may be due to the chemical and physical properties of the surface of the PET ligament.…”

Section: Resultsmentioning

confidence: 99%

Preparation and in vitro evaluation of a biomimetic nanoscale calcium phosphate coating on a polyethylene terephthalate artificial ligament

Chen

Guo

et al. 2016

Experimental and Therapeutic Medicine

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Abstract. In the present study, a polyethylene terephthalate (PET) artificial ligament was coated with an organic layer-by-layer (LBL) self-assembled template of chitosan and hyaluronic acid, and then incubated in a calcium phosphate (CaP) solution to prepare a biomimetic CaP coating. The surface characterization of the ligament was examined using scanning electron microscopy, atomic force microscopy and energy-dispersive X-ray spectroscopy. The effects of CaP coatings on the osteogenic activity of MC3T3 E1 mouse osteoblastic cells were investigated by evaluating their attachment, proliferation and the relative expression levels of alkaline phosphatase. The results revealed that the organic LBL template on the PET artificial ligament was effective for CaP apatite formation. Following incubation for 72 h, numerous nanoscale CaP apatites were deposited on the PET ligament fibers. In addition, the results of the in vitro culture of MC3T3-E1 mouse osteoblastic cells demonstrated that the CaP coating had a good biocompatibility for cell proliferation and adhesion, and the CaP-coated group had a significantly higher alkaline phosphatase activity compared with the uncoated control group after seven days of cell culture. Collectively, these results demonstrated that the biomimetic nanoscale CaP-coated PET artificial ligaments have potential in bone-tissue engineering.

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“…Several functional groups such as Si-OH, 6),8)-9) Ti-OH, 9),10) Zr-OH, 11) Ta-OH, 12) Nb-OH 13) and COOH 14), 15) have been found to induce the apatite nucleation in SBF. On the basis of these findings, organicinorganic hybrids consisting of organic polymer and the compounds that provide the functional groups above described would be attractive as novel bioactive bone substitutes with mechanical performances analogous to those of natural bone.…”

Section: )-3)mentioning

confidence: 99%

Apatite-forming ability of organic-inorganic hybrids fabricated from glucomannan by chemical modification with alkoxysilane and calcium salt

Morita

Miyazaki

Ishida

et al. 2008

J. Ceram. Soc. Japan

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So-called bioactive ceramics have attractive features such as direct bone-bonding in living body via formation of bone-like apatite layer on their surfaces. However, there is limitation on clinical applications due to their inappropriate mechanical properties such as higher Young's modulus and lower fracture toughness than natural bone. Bioactive ceramics bond to bone through bone-like apatite layer formed on their surfaces by chemical reaction with body fluid. To overcome the problem of bioactive ceramics, hybrid materials have been developed by organic modification of silanol (Si-OH) group and calcium ions, that are basic component of bioactive ceramics. In this study, we prepared organic-inorganic hybrids from glucomannan, a kind of complex polysaccharide, by modification with alkoxysilane and calcium salts. The apatite formation on the hybrids with different alkoxysilane content was examined in simulated body fluid (SBF). All the samples formed the apatite in SBF irrespective of alkoxysilane content, when they were previously treated with CaCl 2 solution. The hybrids had tendency to be fractured into small pieces in SBF and release large amount of Ca 2＋ at low alkoxysilane content. This means that addition of alkoxysilane is effective for not only providing the hybrids with the apatite-forming ability but also improving stability in aqueous environment.

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Apatite Deposition on Polyamide Films Containing Carboxyl Groups in Body Environment

Cited by 52 publications

References 0 publications

Biomineralization on chemically synthesized collagen containing immobilized poly-γ-glutamic acid

Biomineralization on chemically synthesized collagen containing immobilized poly-γ-glutamic acid

Preparation and in vitro evaluation of a biomimetic nanoscale calcium phosphate coating on a polyethylene terephthalate artificial ligament

Apatite-forming ability of organic-inorganic hybrids fabricated from glucomannan by chemical modification with alkoxysilane and calcium salt

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